Pharmacological evaluation and validation for the folkloric use of Oligochaeta ramosa in constipation and diarrhea
DOI:
https://doi.org/10.3329/bjp.v9i4.20566Keywords:
Calcium antagonist, Constipation, Diarrhea, Muscarinic stimulation, Oligochaeta ramosa, Spasmolytic, Spasmogenic,Abstract
Crude extract of Oligochaeta ramosa and its fractions were studied to rationalize its traditional use in GIT disturbance. In spontaneous contracting jejunum preparation, O. ramosa (0.01-1.0 mg/mL) caused a transient spasmogenic effect followed by the spasmolytic effect at higher doses (3.0-10.0 mg/mL). In atropinized jejunum preparation, O. ramosa inhibit the spontaneous and K+ (80 mM)-induced contraction at the similar doses (0.01-1.0 mg/mL), suggesting calcium channel blocking effect. The calcium channel blocking effect was confirmed when pretreatment of tissue with O. ramosa produced a dose-dependent shift in Ca+2 dose- response curve to the right, similar to that produced by the verapamil. Activity-directed fractionation revealed that the spasmolytic effect is concentrated in the dichloromethane fraction while, aqueous fraction contains both spasmogenic and spasmolytic constituents. This study validate the presence of both spasmogenic and spasmolytic components mediated through muscarinic receptor activation and calcium channel blockade respectively, which may explain its traditional uses in constipation and diarrhea.
Downloads
398
138 Read
284
References
Arunlakhshana O, Schild HO. Some quantitative uses of drug antagonists. British Journal of Pharmacology, 1959; 14: 48-58.
Bhattacharjee SK. Medicinal herbs and flowers. 1st ed. Jaipur, India, Aavishkar Publishers, 2005, p 36.
Bolton TB. mechanism of action of transmitters and other substances on smooth muscles. Physiol Rev. 1979; 59: 606-718.
Brown JH, Taylor P. Muscarinic receptor agonists and antagonists. In: Goodman & Gillmans: The Pharmacological Basis of Therapeutics. Gilman AG, Hardman JG, Limbird LE, Molinoff PB, Ruddon RW (eds.). New York, McGraw-Hill, 1996, pp 141-59.
Chiu AD, McCall DE, Timmermans S. Pharmacological characteristics of receptor-opertard and potential operated calcium channels in rat aorta. Eur J Pharmacol. 1986; 127: 1-8.
Evans WC. Phytochemistry. In: Trease and Evans pharmacognosy. 5th ed. Delhi, Elsevier, 2006, pp 13550.
Farre AJ, Colombo M, Fort M, Gutierrez B. Differential effects of various Ca2+ antagonists. Gen Pharmacol. 1991; 2: 177-81.
Fleckenstein A. Specific pharmacology of Ca2+ in myocardium, cardiac pacemakers and vascular smooth muscle. Rev Pharmacol Toxicol. 1977; 17: 149-66.
Forgacs P, Desconclois JF, Dubec J. Flavones and sesquiterpene lactones of Volutarelladivaricata. Planta Medica. 1981; 42: 284-87
Gilani AH, Aziz N, Khurram IM, Rao ZA, Ali BA. The Presence of cholinomimetic and calcium antagonist consti-tuents in Piper betle Linn. Phytother Res. 2000; 14: 338-44.
Gilani AH, Bashir S, Janbaz KH, Khan A. Pharmacological basis for the use of Fumariaindicain constipation and diarrhea. J Ethnopharmacol. 2005; 96: 585-89.
Gilani AH, Janbaz KH, Zaman M, Lateef A, Suri, A, Ahmed HR. Possible presence of calcium channel blocker(s) in Rubiacordifolia: An indigenous medicinal plant. J Pakistan Med Assoc. 1994; 44: 82-85.
Godfraind T, Miller R, Wibo M. Calcium antagonism and calcium entry blockade. Pharmacol Rev. 1986; 38: 321-416.
Hamilton TC, Weir SW, Weston AH. Comparison of the effect of BRL 34915 and verapamil on electrical and mechanical activity on rat portal vain. Br J Pharmacol. 1986; 88: 103-11.
Hussain M, Bakhsh H, Aziz A, Majeed A, Khan IA, Mujeeb A, Farooq U. Comparative In vitro study of antimicrobial activities of flower and whole plant of Jasminum officinale against some human pathogenic microbes. J Pharma Alternat Med. 2013; 2: 33-43.
Hussain M, Raza SM, Farooq U, Bakhsh H, Majeed A, Aziz A. In vitro Antimicrobial potential of lichen (Parmelia perlata) against different pathogenic microbes. Int J Pharma Sci. 2014; 4: 666-70.
Ibrahim M, Imran M, Ali B, Hussain R, Rehmani FS, Malik A. A secondary metabolite amberin from Amberboa ramosa. J Asian Nat Prod Res. 2012; 14: 281-85.
Ibrahim M, Khan R, Malik A. Two new guaianolides from Amberboa ramosa. Natl Prod Comm. 2010; 5: 1865-68.
Jalapure SS, Habbu PV, Patil MB, Kulkani RV, Simpi CC, Patil CC. Analgesic and antipyretic activity of Pergularia extensa in rats. Indian J Pharmaceut Sci. 2002; 6: 493-95.
Khan SB, Afza N, Malik A, Haq AU, Ahmed Z. Structure determination of ramosine, a guaianolide, by NMR spectroscopy. Magn Reson Chem. 2004; 42: 1063-65.
Khan SB, Haq AU, Afza N, Malik A, Khan MT, Shah MR, Choudhary MI. Tyrosinase-inhibitory long-chain esters from Amberboa ramosa. Chem Pharm Bull. 2005a; 53: 86-89.
Khan SB, Haq AU, Perveen S, Afza N, Malik A, Nawaz SA, Shah MR, Choudhary MI. Butyryl-cholinesterase inhibitory guaianolides from Amberboa ramosa. Arch Pharm Res. 2005b; 28: 172-76.
Khare CP. Indian Medicinal plants: An illustrated dictionary. New Delhi, India, Springer, Berlin/ Heidelberg, 2007, pp 42, 712-13.
Kiritikar KR, Basu BD. Indian medicinal plants. Blatter E, Caius JF, Mahaskr KS (eds). Dehradun, India, Vol III , 2nd ed., 1987, pp 1961-63, 1023-28.
National Research Council. Guide for the care and use of laboratory animals., Washington, DC, USA, National Academy Press, 1996.
Qureshi R, Bhatti GR. Ethnobotany of plants used by the Thari people of Nara Desert, Pakistan. Fitoterapia. 2008; 79: 468-73.
Sharif NA, Xu SX, Yanni JM. Histamine receptor-subtype affinities, selectivities, and potencies of emedastine, a novel Hi-selective antagonist, and other ocularly employed antihistamines. Drug Develop Res. 1994; 33: 448-53.
Van-Rossum JM. Cumulative concentration-response curves Techniques for making concentration response curves in isolated organs and evaluation of drug parameters. Arch Int Pharmacodyn Ther. 1963; 143: 229-33.
Vardhana R. Direct use of medicinal plants and their identification.. UP, India, 2008, pp 29-30.
Williamson EM, Okpako DT, Evans FJ. Pharmacological methods in phytotherapy research. John Wiley & Sons, Chichester, 1963, pp 15-23.
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
