Anti-cancer potential of banana flower extract: An in vitro study
DOI:
https://doi.org/10.3329/bjp.v9i4.20610Keywords:
Anti-cancer, antiproliferative, banana flower, bioactive fraction, cytotoxic, HeLa cellsAbstract
Banana (Musa paradisiaca) flower is rich in phytochemicals (vitamins, flavonoids, proteins) and has antioxidant properties. The anti-cancer activity of banana flower extract has been evaluated on the cervical cancer cell line HeLa. The antiproliferative effects were evaluated by MTT assay. The extract was further purified by TLC and characterized by LC-MS method. The ethanol extract had significant cytotoxicity to HeLa cells with an IC50 of 20 µg/mL. By thin layer chromatography we could isolate three fractions out of which fraction 2 had exhibited maximum anti-proliferative effects with an IC50 value of <10 µg/mL. By LC-MS analysis, bioactive fraction was found to have an m/z value of 224.2 indicating it as a novel one.
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References
Bhaskar JJ, Salimath PV, Nandini CD. Stimulation of glucose uptake by Musa sp. (cv. Elakkibale) flower and pseudostem extracts in Ehrlich ascites tumor cells. J Sci Food Agric. 2011; 91: 1482-87.
Chen MS, Chen D, Dou QP. Inhibition of proteasome activity by various fruits and vegetables is associated with cancer cell death. In Vivo. 2004; 18: 7380.
Cohen JJ. Apoptosis. Immunol Today. 1993; 14: 126-30.
China R, Dutta S, Sen S, Chakrabarti R, Bhowmik D, Ghosh S, Dhar P. In vitro antioxidant activity of different cultivars of banana flower (Musa paradicicus L.) extracts available in India. J Food Sci. 2011; 76: 1292-99.
Debabandya M, Sabyasachi M, Namrata S. Banana and its byproducts utilization: An overview. J Sci Indian Res. 2010; 69: 32329.
Deneo-Pellegrini H, De Stefani E, RoncoA, MendilaharsuM, CarzoglioJC. Meat consumption and risk of cancer: A case control study from Uruguay. Lung Cancer. 1996; 14: 195-205.
Harborne JB. Phytochemicals methods. London, Chapman and Hall Ltd., 1973, pp 49-188.
Kampa M, NifliAP, NotasG, CastanasE. Polyphenols and cancer cell growth. Rev Physiol Biochem Pharmacol. 2007; 159: 79-113.
Kanazawa K, Sakakibara H. High content of dopamine, a strong antioxidant, in Cavendish banana. J Agric Food Chem. 2000; 48: 844-48.
Kirtikar KR, Basu BD. Musa paradisiaca in Indian medicinal plant. Vol. 4. Delhi, Rexiodical Experts Book Agency, 1991, pp 1048-55.
Larkin T. Herbs are often more toxic than magical. FDA Consum. 1983; 17:4-11.
Mallick C, Kausik C, Mehuli GB, Debidas G. Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Cocciniaindica in streptozotocin-induced diabetic male albino rat. Afr J Trad CAM. 2007; 4: 36271.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assays. J Immunol Methods. 1983;16: 55-63.
Mukherjee AK, Basu S, Sarkar N. Advances in cancer therapy with plant based natural products. Curr Med Chem. 2001; 8: 146786.
Pari L, Uma-Maheswari J. Hypoglycaemic effects of Musa sapientum L. in alloxan-induced diabetic rats. J Ethnopharmacol. 1999; 68: 321-25.
Poonguzhali PK, H Cheju. The influence of banana stems extract on urinary risk factors for stones in normal and hyperoxaluric rats. Br J Urol. 1994; 741: 23-25.
Ramos S. Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways. MolNutr Food Res. 2008; 52: 507-26.
Rashidkhani BP, Lindblad A Wok. Fruits, vegetables and risk of renal cell carcinoma: A prospective study of Swedish women. Int J Cancer. 2005; 113: 451-55.
Saxe TG. Toxicity of medicinal herbal preparations. Am Fam Physician. 1987; 35: 135-42.
Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer. 2003; 10: 76880.
Yusoff NAB. Correlation between total phenolics and mineral content with antioxidant activity and determination of bioactive compounds in various local bananas (Musa sp.) Penang, University Sains Malaysia, 2008.
Zhang CX, Suzanne CH,Yu-Ming Chen, Jian-Hua Fu, Shou-Zhen Cheng, Fang-Yu Lin. Greater vegetable and fruit intake is associated with a lower risk of breast cancer among Chinese women. Int J Cancer. 2009; 125: 18188.
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