Vitexicarpin inhibits overexpression of GNAO1 and plays a role in gastric cancer cell proliferation and apoptosis

Authors

  • Si-Ping Wang Domestic Department of Health Management Institute, the Chinese People?s Liberation Army General Hospital, Beijing
  • Li Yu Domestic Department of Health Management Institute, the Chinese People?s Liberation Army General Hospital, Beijing
  • Jie Xie Domestic Department of Health Management Institute, the Chinese People?s Liberation Army General Hospital, Beijing
  • Xiao-Ming Liu Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing
  • Hong Li Domestic Department of Health Management Institute, the Chinese People?s Liberation Army General Hospital, Beijing

DOI:

https://doi.org/10.3329/bjp.v10i1.20975

Keywords:

Proliferation, Aneuploidy, Migration, Therapeutic

Abstract

The present study demonstrates the effect of vitexicarpin on down-regulation of GNAO1 in human gastric cancer cell lines. The results from Western blot analysis revealed that vitexicarpin treatment inhibited the GNAO1 expression in MKN-45 cells at a concentration of 30 µM. Examination of cell proliferation using CCK-8 cell proliferation kit showed 70% reduction in the vitexicarpin treated cells compared to untreated control cells. Cell cycle analysis using propidium Iodide staining followed by flow cytometry showed cell cycle arrest at G0/G1 with reduction of cell population in the S-phase. There was a significant aneuploidy in the controls compared to zero aneuploidy in the GNAO1 down-regulated cells. Transwell chamber and scratch wound healing assay respectively showed 65% and 42% reduction in migration of vitexicarpin treatedMKN-45 cells. Therefore, vitexicarpin treated inhibition of GNAO1can be a potential therapeutic strategy for the treatment of gastric cancer.

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Published

2015-01-20

How to Cite

Wang, S.-P., L. Yu, J. Xie, X.-M. Liu, and H. Li. “Vitexicarpin Inhibits Overexpression of GNAO1 and Plays a Role in Gastric Cancer Cell Proliferation and Apoptosis”. Bangladesh Journal of Pharmacology, vol. 10, no. 1, Jan. 2015, pp. 63-68, doi:10.3329/bjp.v10i1.20975.

Issue

Vol. 10 No. 1 (2015)

Section

Research Articles

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