Screening of Ranunculus sceleratus for enzyme inhibition, antibacterial and antioxidant activities

Authors

  • Sammia Shahid Department of Chemistry, School of Science and Technology, University of Management and Technology, Lahore 54770, Pakistan
  • Tauheeda Riaz Directorate of Land Reclamation, Punjab Irrigation Department, Lahore 54770, Pakistan
  • Muhammad Nadeem Asghar Department of Chemistry, FCC University, Lahore 54000, Pakistan

DOI:

https://doi.org/10.3329/bjp.v10i2.22805

Keywords:

Cholinesterase, DPPH radical, ?-Glucosidase, Lipoxygenase, MBC, MIC

Abstract

Enzyme inhibition potential of various fractions of Ranunculus sceleratus was checked against ?-glucosidase, butyrylcholinesterase, acetylcholinesterase and lipoxygenase enzymes. n-Butanol fraction showed very good activity (77.5 ± 1.0% inhibition at 0.1 mg/mL) against ?-glucosidase. Its IC50 value was 35.7 ± 1.0 µg/mL comparable to quercetin (IC50 value 16.5 ± 0.4 µg/mL). Antibacterial activity was checked against five bacterial strains by 96-wells microplate assay using ciprofloxacin, a standard antibiotic. Chloroform, ethyl acetate, n-butanol and aqueous fractions showed excellent activity against Pseudomonas aeruginosa, (MIC at 7.1, 7.8, 5.6 and 5.3 respectively), which is greater than standard antibiotic ciprofloxacin (MIC 10.0). The antioxidant potential of all the fractions was evaluated. Ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 80.9 ± 1.2% inhibition of DPPH radical at a concentration of 30 µg/mL. These results suggest that R. sceleratus is a valuable herb, which inhibits the oxidative stress mechanism that lead to degenerative diseases.  

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Author Biographies

Sammia Shahid, Department of Chemistry, School of Science and Technology, University of Management and Technology, Lahore 54770, Pakistan

Assistant Professor

 

Tauheeda Riaz, Directorate of Land Reclamation, Punjab Irrigation Department, Lahore 54770, Pakistan

Research Officer

 

Muhammad Nadeem Asghar, Department of Chemistry, FCC University, Lahore 54000, Pakistan

Assistant Professor

 

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Additional Files

Published

2015-05-19

How to Cite

Shahid, S., T. Riaz, and M. N. Asghar. “Screening of Ranunculus Sceleratus for Enzyme Inhibition, Antibacterial and Antioxidant Activities”. Bangladesh Journal of Pharmacology, vol. 10, no. 2, May 2015, pp. 436-42, doi:10.3329/bjp.v10i2.22805.

Issue

Vol. 10 No. 2 (2015)

Section

Research Articles

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