GC/MS analysis, free radical scavenging, anticancer and beta-glucuronidase inhibitory activities of Trillium govanianum rhizome

Authors

  • Shafiq ur Rahman Department of Pharmacy, University of Peshawar, PeshawarDepartment of Pharmacy, ShaheedBenazir Bhutto University, Sheringal, Dir UpperH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
  • Muhammad Ismail Department of Pharmacy, University of Peshawar, Peshawar, Pakistan
  • Muhammad Raza Shah H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
  • Marcello Iriti Department of Agricultural and Environmental Sciences, Milan State University, Milan, Italy
  • Muhammad Shahid Department of Pharmacy, University of Peshawar, Peshawar, Pakistan

DOI:

https://doi.org/10.3329/bjp.v10i3.23446

Keywords:

Anticancer, Antioxidant, GC-MS analysis, ?-Glucuronidase, Trillium govanianum

Abstract

The current study aims to investigate the phytochemical constituents, antioxidant, β-glucuronidase inhibitory and anticancer activities of Trillium govanianum rhizome. Phytochemical screening revealed the presence of steroidal glycosides, saponins, sterols, flavonoids and carbohydrates. GC/MS analyses of n-hexane fraction identified 12 compounds, including 70% unsaturated and 30% saturated fatty acids. Higher free radical scavenging capacity was observed in n-hexane and chloroform fraction compared with the other fractions. Based on IC50 (μg/mL) values, antiproliferative activity on HeLa cells was observed for  chloroform (0.8 ± 0.2), ethyl acetate (1.4 ± 0.1) and butanol (1.6 ± 0.3) fractions by comparison to anticancer drug doxorubicin (0.3 ± 0.0). Similarly, all fractions exhibited cytotoxicity on PC-3 cells. Moreover, the methanol extract (IC50: 140.8 ± 3.8) and butanol fraction (196.2 ± 1.9) exhibited a moderate level of β-glucuronidase inhibitory activity. These findings may validate the folkloric uses of T. govanianum rhizome in cancer management, and can be a promising candidate as an anticancer agent.

Downloads

Download data is not yet available.
Abstract
500
Download
406 Read
232

Downloads

Additional Files

Published

2015-07-05

How to Cite

Rahman, S. ur, M. Ismail, M. R. Shah, M. Iriti, and M. Shahid. “GC/MS Analysis, Free Radical Scavenging, Anticancer and Beta-Glucuronidase Inhibitory Activities of Trillium Govanianum Rhizome”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 577-83, doi:10.3329/bjp.v10i3.23446.

Issue

Vol. 10 No. 3 (2015)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).