Estimation of arsenic in nail using silver diethyldithiocarbamate method

Authors

  • Habiba Akhter Bhuiyan Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • Kipchu Tshering Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • Mir Misbahuddin Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka http://orcid.org/0000-0003-4667-1005

DOI:

https://doi.org/10.3329/bjp.v10i3.23644

Keywords:

Arsenic, Nail, Silver diethyldithiocarbamate, Arsine

Abstract

Spectrophotometric method of arsenic estimation in nails has four steps: a) washing of nails, b) digestion of nails, c) arsenic generation, and finally d) reading absorbance using spectrophotometer. Although the method is a cheapest one, widely used and effective, it is time consuming, laborious and need caution while using four acids.  

Video Clips

Nail wash:                 3.2 min

Digestion of nail:        7.0 min

Arsine generation:     10.0 min

Reading absorbance:   1.2 min 

 

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Author Biographies

Habiba Akhter Bhuiyan, Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

Scientific Officer

Kipchu Tshering, Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

MD student

Mir Misbahuddin, Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

Professor

Google Scholar Citation Index

 

References

Chen KLB, Amarasirlwardena CJ, Christian DC. Determination of total arsenic concentrations in nails by inductively coupled plasma mass spectrometry. Biol Trace Element Res. 1999; 67: 109-25.

Karagas MR, Morris JS, Weiss JE, Spate V, Baskett C, Greenberg ER. Toenail samples as an indicator of drinking water arsenic exposure. Cancer Epidemiol Biomarkers Prev. 1996; 5: 849-52.

Misbahuddin M. Arsenicosis: A global issue. California, Science Publishing Group, 2015.

Schmitt MT, Schreinemachers D, Wu K, Ning Z, Zhao B, Le XC, Mumford JL. Human nails as a biomarker of arsenic exposure from well water in Inner Mongolia: Comparing atomic fluorescence spectrometry and neutron activation analysis. Biomarkers. 2005; 10: 95-104.

Slotnick MJ, Meliker JR, AvRuskin GA, Ghosh D, Nriagu JO. Toenails as a biomarker of inorganic arsenic intake from drinking water and foods. J Toxicol Environ Health, Part A. 2007; 70: 148-58.

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Published

2015-07-01

How to Cite

Bhuiyan, H. A., K. Tshering, and M. Misbahuddin. “Estimation of Arsenic in Nail Using Silver Diethyldithiocarbamate Method”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 513-7, doi:10.3329/bjp.v10i3.23644.

Issue

Vol. 10 No. 3 (2015)

Section

Visual Experiment

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