Antidiarrheal activity of methanolic leaf extract of Rumex vesicarius
DOI:
https://doi.org/10.3329/bjp.v11i1.24251Keywords:
Antidiarrhea, Rumex vesicariusAbstract
This study evaluates the antidiarrheal activity of Rumex vesicarius (leaf) by using in vitro and in vivo assays. Antidiarrheal effect of R. vesicarius was evaluated using castor oil-induced diarrhea model in rat. Weight and volume of the intestinal content were assessed using the enteropooling method. Atropine (3 mg/kg, i.p) was used as positive control. R. Vesicarius at the doses of 200 and 400 mg/kg p.o. significantly retarded castor oil-induced enteropooling and intestinal transit. The gastrointestinal transit rate was studied and R. vesicarius at the doses of 200 and 400 mg/kg significantly inhibited (p<0.001) weight and volume of intestinal content. R. vesicarius caused concentration-dependent (0.011?mg/mL) relaxation of spontaneous contractions in isolated rabbit jejunum tissue preparation and inhibited K+-80 induced contractions (0.01-5?mg/mL), similar to verapamil, suggestive of calcium channel blockade. Results obtained herein indicate that R. vesicarius may contain effective compounds which can be used as an antidiarrheal agent.
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Additional Files
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- Figure 1: Concentration response curves showing the inhibitory effect of R. vesicarius (A) and verapamil (B) on spontaneous and K+ (80 mM)-induced contractions in isolated rabbit jejunum preparations
- Figure 2: Effect of the R. vesicarius on concentration-response curve of Ca2+ on isolated rabbit jejunum preparations (A), and effect of the verapamil (standard) on concentration?response curve of Ca2+ on isolated rabbit jejunum preparations (B)
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