Alloimperatorin and its epoxide derivative exhibit in vitro antitumor activity in HL-60 acute myeloid leukemia cancer cells via inducing apoptosis, cell cycle disruption and inhibition of cell migration
DOI:
https://doi.org/10.3329/bjp.v11i1.24575Keywords:
Acute myeloid leukemia cancer cell, Alloimperatorin, Antitumor, Apoptosis, Cell cycle disruption, Cell migration, Epoxide, HL-60Abstract
The aim of the present study was to synthesize epoxide derivative of alloimperatorin and evaluating its antitumor and apoptotic effects in acute myeloid leukemia HL-60 cells. The cytotoxic effects were demonstrated by MTT assay. Fluorescence microscopy along with flow cytometry were performed to evaluate the effect of alloimperatorin epoxide on apoptosis and cell cycle. In vitro wound healing assay was performed to study compounds effect on cancer cell migration. The results indicated that alloimperatorin epoxide (IC50 = 32.1 µM) was much more effective in inhibiting HL-60 cancer cell growth as compared to alloimperatorin (IC50 = 128 µM). Further, alloimperatorin epoxide induced apoptosis related morphological alterations in HL-60 cells including blebbing of plasma membrane, DNA fragmentation and formation of apoptotic bodies. Alloimperatorin epoxide also led to G2/M phase cell cycle arrest and suppressed HL-60 cancer cell migration indicating that this compound may be a promising candidate for the treatment of cancer metastasis.
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Additional Files
- sound
- Figure 1: Synthetic procedure for the synthesis of epoxide derivative of alloimperatorin using m-chloroperbenzoic acid in dichloromethane
- Figure 2: Cytotoxic effect of alloimperatorin and alloimperatorin epoxide in human leukemia cells (HL-60)
- Figure 3: Fluorescence microscopic study of HL-60 cells using acridine orange/propidium iodide staining method
- Figure 4: Effect of alloimperatorin epoxide on cell cycle progression in HL-60 human leukemia cells
- Figure 5: Alloimperatorin epoxide inhibits HL-60 cancer cell migration
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