Antitumor and apoptotic effects of bergaptol are mediated via mitochondrial death pathway and cell cycle arrest in human breast carcinoma cells

Authors

  • Zhi-Cheng Ge Department of General Surgery, Beijing Friendship Hospital, Capital University of Medical Science, Beijing 100050
  • Xiang Qu Department of General Surgery, Beijing Friendship Hospital, Capital University of Medical Science, Beijing 100050
  • He-Fen Yu Department of Biochemistry and Molecular Biology, School of Basic Medicine, Capital Medical University; Cancer Institute of Capital Medical University; Beijing Key Laboratory for Cancer Invasion and Metastasis Research. Beijing 100069
  • Hui-Ming Zhang Department of General Surgery, Beijing Friendship Hospital, Capital University of Medical Science, Beijing 100050
  • Zi-Han Wang Department of General Surgery, Beijing Friendship Hospital, Capital University of Medical Science, Beijing 100050
  • Zhong-Tao Zhang Department of General Surgery, Beijing Friendship Hospital, Capital University of Medical Science, Beijing 100050 http://orcid.org/0000-0002-0758-6476

DOI:

https://doi.org/10.3329/bjp.v11i2.24644

Keywords:

Antitumor, Apoptosis, Bergaptol, Cell cycle arrest, Human breast carcinoma cell, Mitochondria

Abstract

The aim of the present research work was to investigate the anti-cancer and apoptotic effects of bergaptol in human breast cancer cells (MCF-7). The effects on cell cycle arrest and caspase activation were evaluated. MTT assay was used to evaluate the effect of the compound on cell viability. Cellular morphology was demonstrated by fluorescence microscopy. Flow cytometry was used to analyze effect of bergaptol on cell cycle and apoptosis. The results revealed that bergaptol induced dose-dependent cytotoxic effect on MCF-7 cell viability showing IC50 value of 52.2 µM. Bergaptol induced both early and late apoptosis in concentration-dependent manner. After treatment with bergaptol, an increase in the proportion of cells in the S-phase (37.2, 45.3 and 65.1% as compared to 28.6% in untreated cells) and a reduction in the fraction of cells in the G1 phase (44.1, 41.6 and 35.2% as compared to 51.2% in the untreated cells) was observed.

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References

Böhm I. Disruption of the cytoskeleton after apoptosis induction by autoantibodies. Autoimmunity 2003; 36: 18389.

Conforti F, Menichini F. Phenolic compounds from plants as nitric oxide production inhibitors. Curr Med Chem. 2011; 18: 1137-45.

Eccles SA, Aboagye EO, Ali S, Anderson AS, Armes J, Berditchevski F, Blaydes JP, Brennan K, Brown NJ, Bryant HE, Critical research gaps and translational priorities for the successful prevention and treatment of breast cancer, Breast Cancer Res. 2013; 15: R92.

Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol. 2007; 35: 495-516.

Guzman-Villanueva D, El-Sherbiny IM, Herrera-Ruiz D, Smyth HD. Design and in vitro evaluation of a new nano-microparticulate system for enhanced aqueous-phase solubility of curcumin. Biomed Res Int. 2013; 2013: 724763.

Jemal, A, Bray F, Center MM, Felay J, Ward E, Forman D. Global cancer statistics. CA-Cancer J Clin. 2011; 61: 6990.

Khoo BY, Chua SL, Balaram P. Apoptotic effects of chrysin in human cancer cell lines. Int J Mol Sci. 2010; 11: 218899.

Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nat Rev Drug Discov. 2005; 4: 206-20.

Ma L, Wen ZS, Liu Z, Hu Z, Ma J, Chen XQ, Liu YQ, Pu JX, Xiao WL, Sun HD, Zhou GB. Overexpression and small molecule-triggered down regulation of CIP2A in lung cancer. PLoS One. 2011; 6: e20159.

Molinari G. Natural products in drug discovery: Present status and perspectives. Adv Exp Med Biol. 2009; 655: 13-27.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007; 70: 46177.

Ponci V, Figueiredo CR, Massaoka MH, de Farias CF, Matsuo AL, Sartorelli P, Lago JH. Neolignans from Nectandra megapotamica (Lauraceae) display in vitro cytotoxic activity and induce apoptosis in leukemia cells. Molecules 2015; 20: 2757-68.

Stanley WL, Vannier SH. Chemical composition of lemon oil. I. Isolation of a series of substituted coumarins. J Am Chem Soc. 1957; 79: 348891.

Susin SA, Daugas E, Ravagnan L, Samejima K, Zamzami N, Loeffler M, Costantini P, Ferri KF, Irinopoulou T, Prévost MC, Brothers G, Mak TW, Penninger J, Earnshaw WC, Kroemer G. Two distinct pathways leading to nuclear apoptosis. J Exp Med. 2000; 192: 57180.

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Published

2016-04-17

How to Cite

Ge, Z.-C., X. Qu, H.-F. Yu, H.-M. Zhang, Z.-H. Wang, and Z.-T. Zhang. “Antitumor and Apoptotic Effects of Bergaptol Are Mediated via Mitochondrial Death Pathway and Cell Cycle Arrest in Human Breast Carcinoma Cells”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, Apr. 2016, pp. 489-94, doi:10.3329/bjp.v11i2.24644.

Issue

Vol. 11 No. 2 (2016)

Section

Research Articles

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