Mouse model of ulcerative colitis using trinitrobenzene sulfonic acid

Authors

  • Irfan Ahmad Rather Department of Applied Microbiology and Biotechnology, Gyeongsan Si, Gyeongsan 712749
  • Vivek K. Bajpai Department of Applied Microbiology and Biotechnology, Gyeongsan Si, Gyeongsan 712749
  • Nam Gyeong-Jun Department of Applied Microbiology and Biotechnology, Gyeongsan Si, Gyeongsan 712749

DOI:

https://doi.org/10.3329/bjp.v10i4.25351

Keywords:

Mesalamine, TNBS, Ulcerative colitis

Abstract

Animal model of intestinal inflammation is of paramount significance that aids in discerning the pathologies underlying ulcerative colitis and Crohns disease, the two clinical presentations of inflammatory bowel disease. The 2,4,6-trinitrobenzene sulfonic acid (TNBS) colitis model represents one such intestinal inflammation-prototype that is generated in susceptible strains of mice through intra-rectal instillation of compound TNBS. In this paper, we demonstrate the experimental induction of TNBS-mediated colitis in a susceptible strain of ICR mice. This can be done by the following steps: a) acclimation, b) induction and c) observation. TNBS-mouse model provides the information in shortest possible time and simultaneously represents a cost effective and highly reproducible model method of studying the pathogenesis of inflammatory bowel disease.

Video Clips of Methodology:

Acclimation and induction of TNBS:   4 min 31 sec   Full Screen   Alternate

Observation and drug administration:   1 min 31 sec   Full Screen   Alternate

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Author Biographies

Irfan Ahmad Rather, Department of Applied Microbiology and Biotechnology, Gyeongsan Si, Gyeongsan 712749

Assistant Professor

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Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Gyeongsan Si, Gyeongsan 712749

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References

Fiorucci S, Wallace JL, Mencarelli A, Distrutti E, Rizzo G, Farneti S, et al. A ?-oxidation-resistant lipoxin A4 analog treats hapten-induced colitis by attenuating inflammation and immune dysfunction. Proc Natl Acad Sci USA. 2004; 101: 1573641.

Cooper HS, Murthy SN, Shah RS, Sedergran DJ. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993; 69: 23849.

Low D, Nguyen DD, Mizoguchi E. Animal models of ulcerative colitis and their application in drug research. Drug Des Dev Ther. 2013; 7: 1341-57.

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Published

2015-10-22

How to Cite

Rather, I. A., V. K. Bajpai, and N. Gyeong-Jun. “Mouse Model of Ulcerative Colitis Using Trinitrobenzene Sulfonic Acid”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Oct. 2015, pp. 860-3, doi:10.3329/bjp.v10i4.25351.

Issue

Vol. 10 No. 4 (2015)

Section

Visual Experiment

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