Eriodictyol-induced anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are associated with cell cycle arrest and modulation of apoptosis-related proteins

Authors

  • Fang Wang Department of Healthy Portion, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011
  • Yu-Hang Wang Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011
  • Jing-Jing Wang Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011
  • He-Lin Xu Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011
  • Chang-Miao Wang Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011 http://orcid.org/0000-0003-0533-2189

DOI:

https://doi.org/10.3329/bjp.v11i2.25549

Keywords:

Anti-cancer, Apoptotic, Cell cycle arrest, Eriodictyol, Hepatocellular carcinoma cell

Abstract

The objective of the present study was to investigate the anti-cancer effects of eriodictyol in human hepatocellular carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along with evaluating its mode of action. Sulforhodamine B assay was used to evaluate the cytotoxic effect of the compound while as fluorescence microscopy was involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow cytometry was used to investigate the effect of eriodictyol on cell cycle while Western blot analysis revealed the effect on apoptosis-related protein expressions. Results indicate that eriodictyol-induced selective and concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12 normal liver cells were very less susceptible to its effect. Eriodictyol-induced apoptosis related morphological changes including chromatin condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest in these cells. Eriodictyol led to up-regulation of Bax and PARP and down-regulation of Bcl-2 protein.

Video Clip of Methodology:

Flow cytometry assay: 35 sec   Click to watch

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Published

2016-03-13

How to Cite

Wang, F., Y.-H. Wang, J.-J. Wang, H.-L. Xu, and C.-M. Wang. “Eriodictyol-Induced Anti-Cancer and Apoptotic Effects in Human Hepatocellular Carcinoma Cells Are Associated With Cell Cycle Arrest and Modulation of Apoptosis-Related Proteins”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, Mar. 2016, pp. 285-91, doi:10.3329/bjp.v11i2.25549.

Issue

Vol. 11 No. 2 (2016)

Section

Research Articles

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