Dual receptors blocked mechanism arbitrates smooth muscles relaxant effect of <i>Polypodium vulgare</i>
DOI:
https://doi.org/10.3329/bjp.v11i2.25777Keywords:
Polypodium vulgare, Receptor, Smooth muscleAbstract
The study was aimed to validate the traditional uses of Polypodium vulgare in disorders associated with smooth muscle contraction and to describe its possible underlying mechanism(s) by using in vitro and in vivo experimental techniques. Rhizomes extract of P. vulgare reversed the high K+ (80 mM) and carbachol (1 µM) mediated contractions in isolated rabbit jejunum (5 and 10 mg/mL), trachea (5 and 10 mg/mL) and urinary bladder (3 and 10 mg/mL), with higher potency against carbachol than high K+, similar to dicyclomine. A rightward shift in carbachol cumulative response curves was observed in the presence of crude extract (1-3 mg/mL), similar to dicyclomine. Crude extract exhibited a dose-dependent (300-500 mg/kg) protective effect against castor oil-induced diarrhea in mice. Presence of dual blacked mechanism behind the smooth muscles relaxant effect of the crude extract, unveil medicinal significance of P. vulgare in GIT, respiratory and urinary bladder disorders.
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Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA. Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complement Altern Med. 2005; 5: 6.
Arunlakhshana O, Schild HO. Some quantitative uses of drug antagonists. Br J Pharmacol. 1959; 14: 4858.
Barnes PJ. Neural mechanisms in asthma. Br Med Bull. 1992; 48: 149-68.
Bolton T. Mechanisms of action of transmitters and other substances on smooth muscle. Physiol Rev. 1979; 59: 606-718.
Borchert VE, Czyborra P, Fetscher C, Goepel M, Michel MC. Extracts from Rhois aromatica and Solidaginis virgaurea inhibit rat and human bladder contraction. Naunyn Schmiedebergs Arch Pharmacol. 2004; 369: 281-86.
Borrelli F, Capasso F, Capasso R, Ascione V, Aviello G, Longo R, Izzo AA. Effect of Boswellia serrata on intestinal motility in rodents: Inhibition of diarrhoea without constipation. Br J Pharmacol. 2006; 148: 553-60.
Brown JH, Taylor P. Cholinergic agonists. In: The pharmacological basis of therapeutics. Brunton LL, Lazo JS, Parker KL (eds). New York, McGraw-Hill, 2006, pp 183-200.
Burks TF. Actions of drugs on gastrointestinal motility. In: physiology of the gastrointestinal tract. Johnson LR (ed). 2nd ed. New York, Raven Press, 1987.
Chaudhary MA, Imran I, Bashir S, Mehmood MH, Rehman N, Gilani AH. Evaluation of gut modulatory and bronchodilator activities of Amaranthus spinosus Linn. BMC Complement Altern Med. 2012; 12: 166.
Dar PA, Sofi G, Jafri MA. Polypodium vulgare linn. A versatile herbal medicine: A review. Int J Pharm Sci Res. 2012; 3: 1616-20.
Downie J, Twiddy D, Awad S. Anti-muscarinic and non-competitive antagonist properties of dicyclomine hydrochloride in isolated human and rabbit bladder muscle. J Pharmacol Exp Ther. 1977; 201: 662-68.
Evans WC, Trease GE. Pharmacognosy. Oxford, Alden Press, 1996.
Farre A, Colombo M, Fort M, Gutierrez B. Differential effects of various Ca2+ antagonists. Gen Pharmacol. 1991; 22: 177-81.
Gilani AH, Mehmood MH, Janbaz KH, Khan AU, Saeed SA. Ethnopharmacological studies on anti-spasmodic and anti-platelet activities of Ficuscarica. J Ethnopharmacol. 2008; 119: 1-5.
Gilani AH, Ghayur MN, Khalid A, Zaheer ul Haq, Choudhary MI, Atta ur Rahman. Presence of anti-spasmodic, anti-diarrheal, anti-secretory, calcium antagonist, acetylcholi-nesterase inhibitory, steroidal alkaloids in Sarcococca saligna. Planta med. 2005; 7: 120-25.
Gilani AH, Aftab K, Ahmed S. Cholinergic actions of crude saponins from Castanospermum australe. Pharmaboil. 1994; 32: 20916.
Godfraind T, Miller R, Wibo M. Calcium antagonism and calcium entry blockade. Pharmacol Rev. 1986; 38: 321-416.
Gross NJ. Anti-cholinergic agents in asthma and COPD. Eur J Pharmacol, 2006; 533: 36-39.
Jenkinson. Classical approaches to the study of drugreceptor interactions. In: Textbook of receptor pharmacology. Foreman JC, Johansen T, (eds). Boca Raton, CRC Press, 2002, 378.
Khan AU, Gilani AH. Anti-spasmodic and bronchodilator activities of Artemisia vulgaris are mediated through dual blockade of muscarinic receptors and calcium influx. J Ethnopharmacol. 2009; 126: 480-86.
Khory RN, Katrak NN. Materia medica of India and their therapeutics. Delhi, Neeraj Publishing House, 1981.
Krishnakumaran A, Schneiderman H. Chemical control of moulting in arthropods. Nature 1968; 220: 601-03.
Mann A, Banso A, Clifford LC. An antifungal property of crude plant extracts from Anogeissus leiocarpus and Terminalia avicennioides. Tanzan J Health Res. 2008; 10: 34-38.
National Research Council. Guide for the Care and Use of Laboratory Animals. Washington DC, National Academy Press, 1996; 15.
Ollgaard B, Tind K. Scandinavian Ferns. Aarhus, Rhodos, 1993; 88-91.
Qureshi HM, Omer MO, Ashraf M, Bukhsh A, Chaudhry MA, Imran MS. Evaluation of anti-histaminic and anti-cholinergic activities of Murraya koenigii Linn. Pak Vet J. 2015; 35: 242-44.
Sanmugapriya E, Venkataraman S. Toxicological investigations on Strychnos potatorum Linn seeds in experimental animal models. J Health Sci. 2006; 52: 339-43.
Souri E, Amin G, Farsam H, Jalalizadeh H, Barezi S. Screening of thirteen medicinal plant extracts for anti-oxidant activity. Iran J Pharm Res. 2008; 7: 149-54.
Rahaman MS, Chaudhary MA, Ahmad B, Alamgeer A. Rationalization of traditional uses of Berberislycium in gastrointestinal disorders. Br J Med Med Res. 2013; 3: 868-79.
Van den Brink FG. Development and first check of a new model of functional synergism and antagonism. Eur J Pharmacol. 1973; 22: 270-78.
Van Rossum J. Cumulative dose-response curves II. Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters. Arch Int Pharmacodyn Ther. 1963; 143: 299.
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