Angiogenesis and expression of vascular endothelial growth factor and endostatin protein in myocardial infarction rat model

Authors

  • Chenglong Zheng Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029 and Beijing Drum Tower Hospital of Traditional Chinese Medicine,13 Dou Fu Chi Hutong, Dongcheng District, Beijing 10009
  • Panchu Yang Comprehensive Section, Liangxiang Hospital, Afflicated Hospital of Yanjing Medical College of Capital University of Medical Science, No. 45, Gongchen North Street, Liangxiang Country, Fangshan District, Beijing 102401
  • Lu Zhang Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029
  • Zhiyong Wang Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029
  • Kun Chen Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029
  • Yue Lan Department of Traditional Chinese Medicine of Fuwai Hospital, CAMS and PUMC,137 Bei Li Shi Lu, Xicheng District, Beijing 10009
  • Xing Zhai Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029
  • Kun Huang Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029
  • Shuwen Guo Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029

DOI:

https://doi.org/10.3329/bjp.v11i0.26416

Keywords:

Angiogenesis, Endostatin protein, Myocardial infarction, Vascular endothelial growth factor

Abstract

The aim of this study was the effects of supplementing qi and herbs that promote neovascularization on angiogenesis and expressions of vascular endothelial growth factor and endostatin protein in experimentally induced acute myocardial infarction in rats. Left anterior descending coronary artery was ligated to establish the acute myocardial infarction model. Microvascular vascular count and expressions of vascular endothelial growth factor and endostatin protein were detected and indexes were detected at 7th, 14th and 28th day. The results showed large doses of qi herbs combined with small doses of activating herbs are superior to other groups in promoting the angiogenesis, increasing vascular endothelial growth factor protein expressions and reducing endostatin protein expressions. It also showed that there was a dose-response effect of supplementing qi and activating blood circulation herbs promoting angiogenesis. The mechanism of supplementing qi and activating blood circulation herbs and combination of both promoted angiogenesis due to possibly to increased vascular endothelial growth factor protein expressions and reduced endostatin protein expressions.

 

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Author Biography

Chenglong Zheng, Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029 and Beijing Drum Tower Hospital of Traditional Chinese Medicine,13 Dou Fu Chi Hutong, Dongcheng District, Beijing 10009

 

 

References

Du XJ, Lei Y,Yang J. Effects of Radix Ginseng and Radix Notoginseng formula on expressions of vascular endothelial growth factor receptor-2 and hypoxia-inducible factor-1 alpha in ischemic myocardium of rats with acute myocardial infarction. Zhong Xi Yi Jie He Xue Bao. 2010; 8: 548-53.

Gao XF, Shi HM, Sun T, Ao H. Effects of Radix et Rhizoma Rhodiolae Kirilowii on expressions of von Willebrand factor, hypoxia-inducible factor1 and vascular endothelial growth factor in myocardium of rats with acute myocardial infarction. Zhong Xi Yi Jie He Xue Bao. 2009; 7: 434-40.

Guo SW, Cui W, Wang SR, Niu FL, Wang GH. Time-phase changed character of cardiac muscle cell apoptosis and proliferation induced by angiotensin II and influences of TCM herbs for supplementing Qi and activating blood circulation on it. JITWM. 2005; 25: 1004-07.

Guo SW, Wang GH, Qin LM, Niu FL, Wang SR, Lu JQ, Liu ZQ, Yan YF. Effects of some Chinese herbs on changes of energy and lipid peroxidation in cardiac cells induced by Angiotensin II. J Beijing Univ Traditional Chinese Med. 2005; 28: 32-35.

Guo SW, Zheng CL, Huang PP, Zhang L, Yang PC, Zhang JF, Zhou JY, Huang K, Yang DD, Sun Q. Effects of supplementing qi and activating blood circulation herbs on expressions of basic fibroblast growth factor and platelet endothelial cell adhesion molecule-1 in ischemic myocardium of rats with acute myocardial infarction. Afr J Pharm Pharmacol. 2012; 6: 2735-41.

Halkos ME, Rab ST, Vassiliades TA, Morris DC, Douglas JS, Kilgo PD, Liberman HA, Guyton RA, Thourani VH, Puskas JD. Hybrid coronary revascularization versus off-pump coronary artery bypass for the treatment of left main coronary stenosis. Ann Thorac Surg. 2011; 92: 2155-60.

Isobe K1, Kuba K, Maejima Y,Suzuki J,Kubota S, Isobe M. Inhibition of endostatin/collagen XVIII deteriorates left ventricular remodeling and heart failure in rat myocardial infarction model. Circ J. 2010; 74: 109-19.

Jazi SM, Esfahani MH, Fesharaki M, Moulavi F, Gharipour M. Initial clinical outcomes of intracoronary infusion of autologous progenitor cells in patients with acute myocardial infarction. ARYA Atheroscler. 2012; 7: 162-67.

Jung S, Wermker K, Poetschik H, Ziebura T, Kleinheinz J.The impact of hyperbaric oxygen therapy on serological values of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF).Head Face Med. 2010; 6: 261-65.

Koyama H. Therapeutic angiogenesis for the treatment of ischemic heart disease. Nihon Rinsho. 2011; 69: 316-19.

Lee KH, Choi SI, Chun EJ, Kim JA, Lee MS, Yoon CH, Choi DJ. Aborted myocardial infarction: Evaluation of changes in area at risk, late gadolinium enhancement, and perfusion over time and comparison with overt myocardial infarction. Am J Roentgenol. 2012; 199: 328-35.

Stanton LW, Garrard LJ, Damm D, Garrick BL, Lam A, Kapoun AM, Zheng Q. Altered patterns of gene-expression in response to myocardial infarction. Circ. Res. 2000; 86: 939-45.

Yu JM, Zhang XB, Jiang W, Wang HD, Zhang YN. Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibro-blast growth factor in a rat model of myocardial infarction. Mol Med Rep. 2015; 12: 6718-26.

Zhao TQ, Zhao WY, Chen YG, Liu L, Ahokas RA, Sun Y. Differential expression of vascular endothelial growth factor isoforms and receptor subtypes in the infarcted heart. Int J Cardiol. 2013; 167: 263845.

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Published

2016-02-13

How to Cite

Zheng, C., P. Yang, L. Zhang, Z. Wang, K. Chen, Y. Lan, X. Zhai, K. Huang, and S. Guo. “Angiogenesis and Expression of Vascular Endothelial Growth Factor and Endostatin Protein in Myocardial Infarction Rat Model”. Bangladesh Journal of Pharmacology, vol. 11, no. S1, Feb. 2016, pp. S1-S7, doi:10.3329/bjp.v11i0.26416.

Issue

Vol. 11 (2016): Special Issue

Section

Research Articles

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