Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats association with increased expression of mTOR
DOI:
https://doi.org/10.3329/bjp.v11iS1.26418Keywords:
Electroacupuncture, Hypoxic-ischemic encephalopathy, mTOR, Neuroprotective, NewbornAbstract
In this study, we observed the therapeutic effects of acupuncture and investigated the underlying molecular mechanisms by constructed a hypoxic-ischemic encephalopathy (HIE) animal model. In the electroacupuncture group, mTOR expression increased since 1d, and continued to rise till the 21st day. All of the differences were significantly (p<0.05 vs the model group). Meanwhile, mTOR expression was analyzed by Western blotting. There was statistical significance between the model group and the electroacupuncture group in the four time periods (p<0.05). The results provide evidence that electroacupuncture treatment protected cortical neurons against HIE-induced neuronal damage and degenerative changes in rats, which is in association with activation of mTOR both at the mRNA level and protein level. Therefore, electroacupuncture may become a potential therapeutic strategy for HIE of newborn.
Downloads
208
105 Read
98
References
Bellot G, Garcia-Medina R, Gounon P, et al. Hypoxia-induced autophagy is mediated through hypoxia-inducible factor induction of BNIP3 and BNIP3L via their BH3 domains. Mol Cell Biol. 2009; 29: 2570-81.
Blagosklonny MV. Hypoxia?MTOR and autophagy: Converging on senescence or quiescence. Autophagy 2013; 9: 260-62.
Blomgren K, Leist M, Groc L, et al. Pathological apoptosis in the developing brain. Apoptosis 2007; 12: 9931010.
Chen A, Lin Z, Lan L, et al. Electroacupuncture at the Quchi and Zusanli acupoints exerts neuroprotective role in cerebral ischemia-reperfusion injured rats via activation of the PI3K/Akt pathway. Int J Mol Med. 2012; 30: 791-96.
Chong ZZ, et al. Mammalian target of rapamycin: Hitting the bull's-eye for neurological disorders. Oxid Med Cell Longev. 2010; 3: 37491.
Chuang CM, Hsieh CL, Li TC, et al. Acupuncture stimulation at Baihui acupoint reduced cerebral infarct and increased dopamine levels in chronic cerebral hypoperfusion and ischemia-reperfusion injured Sprague-Dawley rats. Am J Chin Med. 2007; 35: 779-91.
Fahling M. Cellular oxygen sensing, signalling and how to survive translational arrest in hypoxia. Acta Physiol (Oxf.). 2009; 195: 20530.
Fang Z, Ning J, Xiong C, et al. Effects of electroacupuncture at head points on the function of cerebral motor areas in stroke patients: A PET study. Evid Based Complement Alternat Med. 2012: 902413.
Fatemi A, Wilson MA, Johnston MV. Hyopxic-ischemic encephalopathy in the term infant. Clin Perinatol. 2009; 36: 835-58.
Gao J, Wang S, Wang X, et al. Electroacupuncture enhances cell proliferation and neuronal differentiation in young rat brains. Neurol Sci. 2011; 32: 369-74.
Gunn RM, Hailes HC. Insights into the PI3-K-PKB-mTOR signalling pathway from small molecules. Chem Biol. 2008; 1: 4962.
Hong J, Wu G, Zou Y, et al. Electroacupuncture promotes neurological functional recovery via the retinoic acid signaling pathway in rats following cerebral ischemia-reperfusion injury. Int J Mol Med. 2013; 31: 225-31.
Ji XQ, Zhang ZL. Observation on therapeutic effect of nuchal acupuncture and abdominal acupuncture for treatment of stroke patients with spastic hemiplegia. Zhongguo Zhen Jiu. 2009; 29: 961-65.
Kang J, Rychahou PG, Ishola TA, et al. Nmyc is a novel regulator of PI3K-mediated VEGF expression in neuroblastoma. Oncogene 2008; 27: 39994007.
Nakamura K, Martin KC, Jackson JK, et al. Thiele, brain derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1 alpha in neuroblastoma cells. Cancer Res. 2006; 66: 424955.
Kim MW, Chung YC, Jung HC, et al. Scalp electroacupuncture at the Baihui acupoint (DU 20) improves functional recovery in rats with cerebral ischemia. Neural Regen Res. 2011; 6: 2822-28.
Koike M, Shibata M, Tadakoshi M. Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am J Pathol. 2008; 172: 45469.
Lai MC, Yang SN. Perinatal hypoxic-ischemic encephalopathy. J Biomed Biotechnol. 2011: 2011.
Lan L, Tao J, Chen A, et al. Electroacupuncture exerts anti-inflammatory effects in cerebral ischemia-reperfusion injured rats via suppression of the TLR4/NF-?B pathway. Int J Mol Med. 2013; 31: 75-80.
Li M, Peng J, Song Y, et al. Electroacupuncture combined with transcranial magnetic stimulation improves learning and memory function of rats with cerebral infarction by inhibiting neuron cell apoptosis. J Huazhong Univ Sci Technolog Med Sci. 2012; 32: 746-49.
Li L, Qu Y, Li J, Xiong Y, Mao M, Mu D. Relationship between HIF expression and neuronal apoptosis in neonatal rats with hypoxiaischemia brain injury. Brain Res. 2007: 1180; 13339.
Liu R, Xu N, Yi W, et al. Electroacupuncture effect on neurological behavior and tyrosine kinase-JAK 2 in rats with focal cerebral ischemia. J Tradit Chin Med. 2012; 32: 465-70.
MacPherson H, Hammerschlag R. Acupuncture and the emerging evidence base: Contrived controversy and rational debate. J Acupunct Meridian Stud. 2012; 5: 141-47.
Mazure NM, Pouysségur J. Hypoxia-induced autophagy: Cell death or cell survival? Curr Opin Cell Biol. 2010; 22: 177-80.
Rice JE, Vannucci RC, Brierley JB, et al. The influence of immaturity on hypoxic-ischemic brain damage in the rat. Ann Neurol. 1981; 9: 131-41.
Rudov A, Balduini W, Carloni S, Perrone S, Buonocore G, Cristina Albertini M. Involvement of miRNAs in placental alterations mediated by oxidative stress. Oxid Med Cell Longev. 2014: 1030-68.
Schicknik H, Schott BH, Budinger E, et al. Dopaminergic modulation of auditory cortex-dependent memory consolidation through mTOR. Cereb Cortex. 2008; 18: 2646-58.
Wang GB, Xu NG, She SF, et al. Effect of electroacupuncture on the synaptic structure in the ischemic areas of rats with focal cerebral ischemia. Zhongguo Linchuang Kangfu. 2005; 9: 115-17.
Xue WG, Ge GL, Zhang Z, et al. Effect of electroacupuncture on the behavior and hippocampal ultrastructure in APP 695 V 717 I transgenic mice. Zhen Ci Yan Jiu. 2009; 34: 309-14.
Xue WG, Zhang Z, Xu H, et al. Effect of electroacupuncture on learning-memory ability, and Abeta and LRP1 immunoactivity in hippocampal sulcus microvessels in APP transgenic mice. Zhen Ci Yan Jiu. 2011; 36: 95-100.
Yang ZX, Yu HB, Wang L, et al. Effect of electroacupuncture on synaptic transmission in hippocampal dentate gyrus of rats with cerebral ischemic damage. Guangzhou Zhongyiyao Daxue Xuebao. 2005; 22: 118-22.
Yi W, Xu NG, Wang GB, et al. Experimental study on effects of electroacupuncture in improving synaptic plasticity in focal cerebral ischemia rats. Zhongguo Zhongxiyi Jiehe Zazhi. 2006; 26: 710-14.
Zhao Y, Chen S, Yu W, et al. The effect of electroacupuncture on endogenous EPCs and serum cytokines in cerebral ischemia-reperfusion rat. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010; 27: 1322-26.
Zhou W, Liu H, Wang LP, et al. Effect of alternative administration of scalp-acupuncture, body-acupuncture and abdominal-acupuncture on the upper-limb dyscinesia in patients with stroke. Zhen Ci Yan Jiu. 2009; 34: 128-31.
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
