Isolation of flavonoids from onion skins and their effects on K562 cell viability

Authors

  • Guo-Qing Shi School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002
  • Jing Yang School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002
  • Jiang Liu National Engineering Laboratory for Further Processing of Wheat and Corn, Henan University of Technology, Henan, Zhengzhou 450000
  • Sheng-Nan Liu School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002
  • Han-Xue Song School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002
  • Wen-En Zhao School of Chemical Engineering and Energy, Zhengzhou University, Henan, Zhengzhou 450052
  • Yan-Qi Liu School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002

DOI:

https://doi.org/10.3329/bjp.v11iS1.26419

Keywords:

Flavonoid, K562 cell, Onion skin, Viability

Abstract

To investigate the anti-proliferative activity of flavonoids from onion skins, extraction by 50% ethanol (v/v), soxhlet polar fractionation, pH gradient separation, thin-layer chromatography, and recrystallization methods were used to isolate and purify flavonoids from dry onion skins. Anti-proliferative activities of some flavonoids obtained on leukemia K562 cell line were deter-mined by MTT assay. Results showed that flavonoids of onion skins were mainly in form of quercetin, kaempferol, isorhamnetin, apigenin-7-O-?-D-glucopyranoside, quercetin-3-O-?-D-glucopyranoside, kaempferol-7-O-?-D-glucopyranoside and rutin. Quercetin and kaempferol decreased K562 cell viability, and quercetin had stronger effect. However, isorhamnetin and rutin exhibited certain proliferation-promoting effects. It suggests that ortho hydroxyl groups on B ring of onion flavonoids might be the key structural elements of their cytotoxic effects on K562 cells, and hydroxyl groups in position 3 or carbonyl groups in position 4 might be one of the structural effect elements.

 

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Author Biography

Guo-Qing Shi, School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002

 

 

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Published

2016-02-19

How to Cite

Shi, G.-Q., J. Yang, J. Liu, S.-N. Liu, H.-X. Song, W.-E. Zhao, and Y.-Q. Liu. “Isolation of Flavonoids from Onion Skins and Their Effects on K562 Cell Viability”. Bangladesh Journal of Pharmacology, vol. 11, no. S1, Feb. 2016, pp. S18-S25, doi:10.3329/bjp.v11iS1.26419.

Issue

Vol. 11 (2016): Special Issue

Section

Research Articles

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