Bryostatin I inhibits growth of breast cancer cells through the inhibition of synuclein-A expression

Authors

  • Jun-Xia Sun Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002
  • Yan Yan Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002
  • Jian-Hong Xia Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002
  • Li-Qing Zhou Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002 http://orcid.org/0000-0003-1682-5237

DOI:

https://doi.org/10.3329/bjp.v11i3.26620

Keywords:

Breast cancer cell, Bryostatin I, Synuclein-A

Abstract

The present study was aimed to investigate the effect of bryostatin I on the expression of synuclein-A in breast cancer cells. Western blot analysis showed a significant (p<0.005) reduction in the expression of synuclein-A from a concentration of 20 µM in H3922 cells. The inhibitory effect of bryostatin I on synuclein-A expression was further confirmed by the treatment of H3922 cells with known synuclein-A inhibitor, cytokine oncostatin M. Bryostatin I treatment of H3922 cells also significantly increased their sensitivity to the taxol. Incubation of the cells with 25 µM concentration of bryostatin I followed by treatment with 0.5 ?M concentration of taxol induced apoptosis in 89% cells compared to 9% cells in the taxol alone treated cultures. Treatment of the H3922 cells with bryostatin I at 25 µM concentration led to a significant increase in the activation of histone H1 protein. The results from MTT assay showed a significant decrease in the cell viability from 10 µM concentration of bryostatin I. Thus, bryostatin I inhibits the growth of breast cancer cells through inhibition of synuclein-A expression and can be used for breast cancer treatment.

Video Clip of Methodology:

Cell viability assay: 5 min 14 sec   Click to watch 

Downloads

Download data is not yet available.
Abstract
163
Download
75 Read
79 Feature
52

Downloads

Additional Files

Published

2016-06-13

How to Cite

Sun, J.-X., Y. Yan, J.-H. Xia, and L.-Q. Zhou. “Bryostatin I Inhibits Growth of Breast Cancer Cells through the Inhibition of Synuclein-A Expression”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, June 2016, pp. 615-20, doi:10.3329/bjp.v11i3.26620.

Issue

Vol. 11 No. 3 (2016)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).