Anti-aging activity of Radix polygoni multiflori preparata in aging mice model induced by D-galactose by bioassay method
DOI:
https://doi.org/10.3329/bjp.v11iS1.27010Keywords:
Anti-aging, D-galactose, Mice, Radix polygoni multifloriAbstract
Anti-aging effect of Radix polygoni multiflori preparata in aging mice model was examined by bioassay method and explore new model of quality control of Traditional Chinese Medicine. Model of aging mice induced by D-galactose were used. The activity of senescence associated molecular markers such as superoxide dismutase, malondialdehyde and glutathione peroxidase in serum or homogenate were determined to evaluate the anti-aging activity. The results showed that the polysaccharide content in the 0.4, 0.8 g/mL decoction were 80.1, 160.2 mg/mL respectively. The HPLC results showed that stibene glucoside content in the 0.4 and 0.8 g/mL decoction were 404 and 808 mg/mL respectively. Experimental results showed that 10 week-old mice were the most sensitive to SOD activity in homogenate. In conclusion, Radix polygoni multiflori preparata has anti-aging effect probably by removing ROS.
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References
Brigelius-Flohe R. Tissue-specific functions of individual glutathione peroxidases. Free Radic Biol Med, 1999; 27: 95165.
Bilgili SG, Ozkol H, Karadag AS,Ozkol HU, Seker A, Calka O, Aslan M. Serum paraoxonase activity and oxidative status in subjects with alopecia areata. Cutan Ocul Toxicol, 2013; 32: 29093.
Chen ZR, Lai PL. Application of biological activity test in the identification of Traditional Chinese Medicine. Chinese J Tradit Chinese Med Pharm. 2001; 16: 60-62.
Chen HY, Jang S, Jinn TR, Chang JY, Lu HF, Li FY. Oxygen radical mediated oxidation reactions of an alanine peptide motif-density functional theory and transition state theory study. Chem Cent. 2012; J6: 33.
Gil P, Farinas F, Casado A, Lopez-Fernandez E. Malondialdehyde: A possible marker of ageing. Gerontology 2002; 48: 20914.
Goldberg LD, Crysler C. A single center, pilot, double-blinded, randomized, comparative, prospective clinical study to evaluate improvements in the structure and function of facial skin with tazarotene 0.1% cream alone and in combination with GliSODin(®) skin nutrients advanced anti-aging formula. Clin Cosmet Investig Dermatol. 2014; 7: 139-44.
Liang Z, Chen HB, Yu ZL, Zhao Z. Comparison of raw and processed Radix polygoni multiflori (Hes-hou wu) by high performance liquid chromatography and mass spectrometry. Chinese Med. 2010; 5: 29.
Ma L, Jin C, Li ZL Bioassay and quality evaluation of Radix Isatidis. Chinese J Exper Tradit Medi Foormulae. 2010; 16, 132-36.
Mohammadirad A, Aghamohammadali-Sarraf F, Badiei S, Faraji Z, Hajiaghaee R, Baeeri M, Gholami M, Abdollahi M.. Anti-aging effects of some selected Iranian folk medicinal herbs-biochemical evidences. Iranian J Basic Medi Sci. 2013; 16, 1170-80.
National Pharmacopoeia Committee. Pharmacopoeia of Peoples Republic of China. Beijing, Chemical Industry Press, 2010, pp 164-65.
Prie BE, Voiculescu VM, lonescu-Bozog OB, Petrutescu B, Iosif L, Gaman LE, Clatici VG, Stoian I, Giucaneanu C. Oxidative stress and alopecia areata. J Med Life. 2015; 8(Spec): 43-46.
Rasheed Z, Alzolibani AA, Al-Shobaili HA, Bin Saif G, Al Robaee AA. Biochemical and immunological studies on erythrocytes superoxide dismutase modified by nitric oxide in patients with alopecia areata: Implications in alopecia patchy persistent and alopecia universalis. Immunol Lett. 2014; 160: 5057.
Sun Q, Xiao XH, Jin C, Yan D. Quality control and evaluation mode of traditional Chinese medicine should be devised. J Chinese Med Materials. 2008; 31: 1-4.
Semba RD, Nicklett EJ, Ferrucci L. Does accumulation of advanced glycation end products contribute to the aging phenotype? J Gerontol A Biol Sci Med Sci. 2010; 65: 96375.
Stadtman ER. Protein oxidation and aging. Science 1992; 257: 122024.
Tian J, Ishibashi K, Ishibashi K, Reiser K, Grebe R, Biswai S, Gehlbach P, Handa JT. Advanced glycation end product-induced aging of the retinal pigment epithelium and choroid: A comprehensive transcriptional response. Proc Natl Acad Sci USA. 102: 1184651.
Tan YR. The anti-aging mechanism of Tuber Fleece flower root. Hunan Guiding J TCM, 2004; 10: 73-74.
Wang JB, Jin C, Li HF. Essential issues of bioassay methods for quality control of laxative Traditional Chinese medicines. Acta Pharmaceutica Sinica. 2009; 44: 500-05.
Xu XH, Ping YH, Xie YH. HPLC simultaneous determination of four active ingredients content in Radix polygoni multiflorum. Lishizhen Med Materia Medica Res. 2011; 22: 58-59.
Yao MC, Luo BR, Lan KW, Yang AH, Zhang QS. The study on the anti-aging effect of Radix polygoni multiflori. Chinese Pharmaceut J. 1984; 19: 28-30.
Yang MH, Wang RR. Study on the biological assay of herba leonuri III-related observation of fresh Herba leonuri capsules and Herba leonuri fluid extracts in biological potency and clinical effect. Chinese J Mod Appl Pharm. 2004; 21: 124-26.
Yang MH, Yang SB, Jin ZH, Zhu SY. Study on the biological assay of herba leonuri II: Analysis the dosage response of Herba leonuri and oxytocin and establishment of adequate potency pattern. J Chinese Med Materials. 2002a; 25: 409-11.
Yang MH, Yang SB, Jin ZH, Guo YH . Study on the biological assay of Herba leonuri I- Establishment of standard uterus models and optimizing uterus environment conditions. J Chinese Med Materials. 2002b; 25: 333-36.
Zhao ZJ, Zhang EH, Lian Y, Chen DZ, Wang GQ. Methodological investigation of biological assays and preparations of Hypericum perforatum. Chinese J Hospital Pharm. 2008; 28: 2146-48.
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