Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

Authors

  • Vivek K. Bajpai Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749 http://orcid.org/0000-0003-0423-6195
  • Rajib Majumder Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749
  • Irfan A. Rather Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749 http://orcid.org/0000-0003-2752-0519
  • Kangmin Kim Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si-570752, Joellabuk-do (Jeonbuk)

DOI:

https://doi.org/10.3329/bjp.v11i3.27170

Keywords:

Exopolysaccharide, Lactic acid bacteria

Abstract

Lactic acid bacteria are classified Generally Recognized As Safe (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.

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Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749

Assistant Professor

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Rajib Majumder, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749

Researcher

Irfan A. Rather, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749

Assitant Professor

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Kangmin Kim, Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si-570752, Joellabuk-do (Jeonbuk)

Professor

References

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Published

2016-06-02

How to Cite

Bajpai, V. K., R. Majumder, I. A. Rather, and K. Kim. “Extraction, Isolation and Purification of Exopolysaccharide from Lactic Acid Bacteria Using Ethanol Precipitation Method”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, June 2016, pp. 573-6, doi:10.3329/bjp.v11i3.27170.

Issue

Vol. 11 No. 3 (2016)

Section

Visual Experiment

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