Histopathological analysis of 2,4,5-trinitrobenzene sulphonic acid (TNBS)-induced mouse colitis

Authors

DOI:

https://doi.org/10.3329/bjp.v11i3.28155

Keywords:

Colitis, Histology, Inflammation

Abstract

Ulcerative colitis is one of the chronic diseases that results in inflammation of colon in humans. In this Visual experiment we demonstrate the histopathology of TNBS induced colitis model of mouse. The experiment involves following steps: a) Selection of mouse model, b) Induction of colitis, c) Paraffin sectioning of colon tissue, d) Hematoxylin and Eosin staining, and d) Microscopic observation.

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13 min 14 sec   Full Screen   Alternate

3 min 13 sec   Full Screen   Alternate

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Author Biography

Irfan A. Rather, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan 712749

Assistant Professor

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References

Park SY, Ku SK, Lee ES, Kim JA. 1,3-Diphenylpropenone ameliorates TNBS-induced rat colitis through suppression of NF-?B activation and IL-8 induction. Chem Biol Interact. 2012; 196: 39-49.

Peran L, Camuesco D, Comalada M, Nieto A, Concha A, Diaz-Ropero MP, Olivares M, Xaus J, Zarzuelo A, Galvez J. Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis. World J Gastroenterol. 2005; 11: 5185-92.

Rather IA, Bajpai VR, Gyeong-Jun N. Mouse model of ulcerative colitis using trinitrobenzene sulfonic acid. Bangladesh J Pharmacol. 2015; 10: 860-63.

Satsangi J, Silverberg MS, Vermeire S, et al. The Montreal classification of inflammatory bowel disease: Controversies, consensus, and implication. Gut 2006; 55: 749-53.

Stucchi AF, Shofer S, Leeman S, Materne O, Beer E, McClung J, Shebani K, Moore F, OBrien M, Becker JM. NK-1 antagonist reduces colonic inflammation and oxidative stress in dextran sulfate-induced colitis in rats. Am J Physiol Gastrointest Liver Physiol. 2000; 279: 1298-306.

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Published

2016-06-12

How to Cite

Choi, K.-H., H. Hyeonju, and I. A. Rather. “Histopathological Analysis of 2,4,5-Trinitrobenzene Sulphonic Acid (TNBS)-Induced Mouse Colitis”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, June 2016, pp. 610-4, doi:10.3329/bjp.v11i3.28155.

Issue

Vol. 11 No. 3 (2016)

Section

Visual Experiment

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