Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2

Authors

DOI:

https://doi.org/10.3329/bjp.v12i3.32337

Keywords:

Anti-cancer, Cleome gynandra, DPPH, MTT, Palmitic acid, Phytol, Phenol

Abstract

The aim of the present study was to analyze the anti-cancer effect of Orthosiphon stamineus leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their Rf values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC50 = 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl2, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl2 and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in O. stamineus. The anti-cancer activity of O. stamineus may be due to the presence of elutherol and cinnamic acid.

Video Clip of Methodology:

LC-MS analysis of Orthosiphon stamineus extract: 7 min 33 sec   Full Screen   Alternate 

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Author Biography

Brindha Pemaiah, Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu

Associate Dean and Co-ordinator

Published

2017-07-25

How to Cite

Saravanan, R., B. Pemaiah, S. Sridharan, M. Narayanan, and S. Ramalingam. “Enhanced Cytotoxic Potential of Orthosiphon Stamineus Extract in MCF-7 Cells through Suppression of Nucleolin and Bcl2”. Bangladesh Journal of Pharmacology, vol. 12, no. 3, July 2017, pp. 268-75, doi:10.3329/bjp.v12i3.32337.

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Section

Research Articles