Brine shrimp lethality assay

Authors

  • Quazi Sahely Sarah Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Fatema Chowdhury Anny Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Mir Misbahuddin Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000 http://orcid.org/0000-0003-4667-1005

DOI:

https://doi.org/10.3329/bjp.v12i2.32796

Keywords:

Brine shrimp, Lethality assay

Abstract

Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity assay of plant extract and others based on the ability to kill a laboratory cultured larvae (nauplii). The nauplii were exposed to different concentrations of plant extract for 24 hours. The number of motile nauplii was calculated for the effectiveness of the extract. It is a simple, cost effective and requires small amount of test material.

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Author Biography

Mir Misbahuddin, Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000

Professor

Cell: 880-1849396067; email: dgdabd@gmail.com

ORCHID ID: 0000-0003-4667-1005

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References

Carballo JL, Hernández-Inda ZL, Pérez P, García-Grávalos MD. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnol. 2002; 2: 17.

Galsky AG, Wilsey JP, Powell RG. Crown gall tumor disc bioassay: A possible aid in the detection of compounds with antitumor activity. Plant Physiol. 1980; 65: 184-85.

Ghosh A, Banik S, Islam M. In vitro thrombolytic, anthelmintic, anti-oxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves. Bangladesh J Pharmacol. 2015; 10: 854-59.

Harwing J, Scott PM. Brine shrimp (Artemia salina L.) larvae as a screening system for fungal toxins. Appl Microbiol. 1971; 21: 1011-16.

Hisem D, Hrouzek P, Tomek P, Tomí?ková J, Zapom?lová E, Skácelová K, Lukeová A, Kopecký J. Cyanobacterial cytotoxicity versus toxicity to brine shrimp Artemia salina. Toxicon 2011; 57: 76-83.

Kibiti C, Afolayan A. Antifungal activity and brine shrimp toxicity assessment of Bulbine abyssinica used in the folk medicine in the Eastern Cape Province, South Africa. Bangladesh J Pharmacol. 2016; 11: 469-77.

Maurer-Jones MA, Love SA, Meierhofer S, Marquis BJ, Liu Z, Haynes CL. Toxicity of nanoparticles to brine shrimp: An introduction to nanotoxicity and interdisciplinary science. J Chem Educ. 2013; 90: 475-78.

Mclaughlin JL, Rogers LL, Anderson JE. The uses of biological assays to evaluate botanicals. Drug Inf J. 1998; 32: 513-24.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 1982; 45: 31-34.

Michael AS, Thampson CG, Abramoritz M. Artemia salina as a test organism for bioassay. Science 1956; 123: 464.

Oberlies NH, Rogers LL, Martin JM, McLaughlin JH. Cytotoxic and insecticidal constituents of the unripe fruit of Persea americana. J Nat Prod. 1998; 61: 781-85.

Pelka M, Danzl C, Distler W, Petschelt A. A new screening test for toxicity testing of dental materials. J Dent. 2000; 28: 341-45.

Saliba LJ. Krzyz RM. Effect of heavy metals on hatching of brine-shrimp eggs. Marine Poll Bull. 1976; 7: 181-82.

Spielman A, Williams CM. Lethal effects of synthetic juvenile hormone on larvae of the yellow fever mosquito, Aedes aegypti. Science 1966; 154: 1043-44.

Sufian M, Haque M. Cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities of Hygrophila schulli. Bangladesh J Pharmacol. 2015; 10: 692-96.

Syahmi ARM, Vijayaratna S, Sasidharan S, Latha LY, Kwan YP, Lau YL, Shin LN, Chen Y. Acute oral toxicity and brine shrimp lethality of Elaeis guinennsis Jacq., (oil palm leaf) methanol extract. Molecules 2010; 15: 8111-21.

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Published

2017-06-05

How to Cite

Sarah, Q. S., F. C. Anny, and M. Misbahuddin. “Brine Shrimp Lethality Assay”. Bangladesh Journal of Pharmacology, vol. 12, no. 2, June 2017, p. Available Online: Jun 5, 2017, doi:10.3329/bjp.v12i2.32796.

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Visual Experiment