Estimation of vitamin B12 in plasma by High Performance Liquid Chromatography

Authors

  • Fahmida Hasnat Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Habiba Akhter Bhuiyan Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Mir Misbahuddin Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000 http://orcid.org/0000-0003-4667-1005

DOI:

https://doi.org/10.3329/bjp.v12i3.33108

Keywords:

High Performance Liquid Chromatography, Plasma, Vitamin B12

Abstract

High performance liquid chromatography was used to develop and validate the detection of vitamin B12 in blood plasma sample. The mobile phase consists of a mixture of 0.025% trifluroacetic acid in deionized water and 30% methanol. The mixture used was adjusted to pH 2.9 and the flow rate was adjusted to 0.5 mL/min. The separation was achieved using C18 column maintained at 30ºC temperature and detection of vitamin B12 was conducted at maximum wavelength 230 nm.

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Author Biography

Mir Misbahuddin, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000

Professor

Cell: 880-1849396067; email: dgdabd@gmail.com

ORCHID ID: 0000-0003-4667-1005

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References

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Published

2017-07-12

How to Cite

Hasnat, F., H. A. Bhuiyan, and M. Misbahuddin. “Estimation of Vitamin B12 in Plasma by High Performance Liquid Chromatography”. Bangladesh Journal of Pharmacology, vol. 12, no. 3, July 2017, pp. 251-5, doi:10.3329/bjp.v12i3.33108.

Issue

Vol. 12 No. 3 (2017)

Section

Visual Experiment

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