Breeding of zebrafish in the laboratory environment for research development
DOI:
https://doi.org/10.3329/bjp.v12i4.34143Keywords:
Zebrafish maintenance, breeding, spawning, sub-optimal conditionAbstract
The zebrafish (Danio rerio) has turn out to be an excellent vertebrate organism for analyzing developmental stages, disease modeling, and screening of drug toxicities due to their minuscule, high fecundity, optical transparency and there close parallels with the genome of human beings. Although maintenance of zebrafish is relatively easy, yet it is a complex process, greatly influenced by various factors including mating behavior, day-light cycle, age, and size of fish. This visual experiment will provide an overview of zebrafish care and maintenance in the laboratory environment. Additionally, the presentation will include information essential for improving the spawning habitat by recreating breeding settings in the lab. Collectively, this experimental memorandum of zebrafish environment can serve as a mean to understand the reproducibility, efficiency of use and its welfare for future research development.
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References
Adatto I, Lawrence C, Thompson M, Zon LI. A new system for the rapid collection of large numbers of developmentally staged zebrafish embryos. PLoS One. 2011; 6: e21715.
Avdesh A, Chen M, Martin-Iverson MT, Mondal A, Ong D, Rainey-Smith S, Taddei K, Lardelli M, Groth DM, Verdile G, Martins RN. Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction. J Vis Exp. 2012: 18:4196.
Blackburn JS, Liu S, Raimondi AR, Ignatius MS, Salthouse CD, Langenau DM. High throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope. Nature Protocols. 2011; 6:229-41.
Blaser RE, Chadwick L, McGinnis GC. Behavioral measures of anxiety in zebrafish (Danio rerio). Behav Brain Res. 2010; 208: 56-62.
Doke SK, Dhawale SC. Alternatives to animal testing: A review. Saudi Pharm J. 2015; 23: 223-9.
Eddins D, Petro A, Williams P, Cerutti DT, Levin ED. Nicotine effects on learning in zebrafish: the role of dopaminergic systems. Psychopharmacology (Berl). 2009; 202:103-9.
Gerlai R. Zebra fish: an uncharted behavior genetic model. Behav Genet. 2003; 33: 461-8.
Grunwald DJ, Eisen JS. Headwaters of the zebrafish -- emergence of a new model vertebrate. Nat Rev Genet. 2002; 3: 717-24.
Hoo JY, Kumari Y, Shaikh MF, Hue SM, Goh BH. Zebrafish: A Versatile Animal Model for Fertility Research. Biomed Res Int. 2016; 2016: 9732780.
Howe DG, Bradford YM, Eagle A, Fashena D, Frazer K, Kalita P, Mani P, Martin R, Moxon ST, Paddock H, Pich C, Ramachandran S, Ruzicka L, Schaper K, Shao X, Singer A, Toro S, Van Slyke C, Westerfield M. The Zebrafish Model Organism Database: New support for human disease models, mutation details, gene expression phenotypes and searching. Nucleic Acids Res. 2017; 45: D758-D768.
Kent ML, Buchner C, Barton C, Tanguay RL. Toxicity of chlorine to zebrafish embryos. Dis Aquat Organ. 2014;107: 235-40.
Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. Stages of embryonic development of the zebrafish. Dev Dyn. 1995; 203: 253-310.
Lawrence C. Advances in zebrafish husbandry and management. Methods Cell Biol. 2011; 104: 429-51.
Lin CY, Chiang CY, Tsai HJ. Zebrafish and Medaka: new model organisms for modern biomedical research. J Biomed Sci. 2016; 23: 19.
Martin-Jimenez R1, Campanella M, Russell C. New zebrafish models of neurodegeneration. Curr Neurol Neurosci Rep. 2015; 15:33.
Nasiadka A, Clark MD. Zebrafish breeding in the laboratory environment. ILAR J. 2012; 53:161-8.
Santoriello C, Zon LI. Hooked! Modeling human disease in zebrafish. J Clin Invest. 2012 ; 122: 2337-43.
Schaefer J, Ryan A. Developmental plasticity in the thermal tolerance of zebrafish Danio rerio. J Fish Biol. 2006; 69: 722-734.
Schilling TF. Zebrafish: A practical approach. New York, NY: Oxford University Press, 2002.
Spence R, Gerlach G, Lawrence C, Smith C. The behaviour and ecology of the zebrafish, Danio rerio. Biol Rev Camb Philos Soc. 2008; 83: 13-34.
Spitsbergen J. Imaging neoplasia in zebrafish. Nat Methods. 2007; 4: 548-9.
Van Slyke CE, Bradford YM, Westerfield M, Haendel MA. The zebrafish anatomy and stage ontologies: Representing the anatomy and development of Danio rerio. J Biomed Semantics. 2014; 5: 12.
Veldman MB, Lin S. Zebrafish as a Developmental Model Organism for Pediatric Research. Pediatric Research. 2008; 64: 470-476.
Watts SA, Powell M, D Abramo LR. Fundamental approaches to the study of zebrafish nutrition. ILAR J. 2012; 53:144-60.
Westerfield M. The zebrafish book, 5th Edition; A guide for the laboratory use of zebrafish (Danio rerio) University of Oregon Press; Eugene, Oregon, 2007.
Williams CM, Poole AW. Using zebrafish (Danio rerio) to assess gene function in thrombus formation. Methods Mol Biol. 2012; 788: 305-19.
Zhao S, Huang J, Ye J. A fresh look at zebrafish from the perspective of cancer research. J Exp Clin Cancer Res. 2015; 34:80.
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