Antioxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia

Authors

  • Khadidja Bekhouche Departement of Animal Biology, Faculty of Natural and Life Sciences Unversity of Mentouri 1 Constantine http://orcid.org/0000-0002-1039-6681
  • Tevfik Ozen Department of Chemistry, Faculty of Science and Arts, Ondokuz May?s University, Samsun http://orcid.org/0000-0003-0133-5630
  • Sara Boussaha Research Unit and Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL), Constantine University 1 Constantine
  • Serkan Koldas Plant Research Laboratory, Department of Chemistry, University of Cankiri Karatekin, Cankiri http://orcid.org/0000-0002-7917-4008
  • Semiha Yenigun Department of Chemistry, Faculty of Science and Arts, Ondokuz May?s University, Samsun http://orcid.org/0000-0002-1979-5427
  • Somia Lassed Departement of Animal Biology, Faculty of Natural and Life Sciences Unversity of Mentouri 1 Constantine http://orcid.org/0000-0003-1684-880X
  • Ibrahim Demirtas Plant Research Laboratory, Department of Chemistry, University of Cankiri Karatekin, Cankiri http://orcid.org/0000-0001-8946-647X
  • Fadila Benayache Research Unit and Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL), Constantine University 1 Constantine http://orcid.org/0000-0002-2282-6343
  • Samir Benayache Research Unit and Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL), Constantine University 1 Constantine http://orcid.org/0000-0003-2298-2466
  • Djamila Zama Departement of Animal Biology, Faculty of Natural and Life Sciences Unversity of Mentouri 1 Constantine

DOI:

https://doi.org/10.3329/bjp.v13i1.34255

Keywords:

Anti-cancer, Antioxidant, DNA-damage, Perralderia coronopifolia

Abstract

This study was designed to evaluate in vitro the total phenolic, flavonoid content, antioxidant activity, oxidative DNA-damage protection and anti-cancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H2O2-induced oxidative damage. The antiproliferative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH· scavenging ability with IC50= 7.02 ± 0.02 µg/mL. As it shown an oxidative plasmid DNA-damage inhibition against UV-photolysis of H2O2, an anti-cancer activity athigher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness.

Video Component of Methodology:

Metal chelating activity:   2 min 56 sec   Click to watch

Downloads

Download data is not yet available.
Abstract
28845
Download
1349 Read
771

Downloads

Additional Files

Published

2018-03-15

How to Cite

Bekhouche, K., T. Ozen, S. Boussaha, S. Koldas, S. Yenigun, S. Lassed, I. Demirtas, F. Benayache, S. Benayache, and D. Zama. “Antioxidant, DNA-Damage Protection and Anti-Cancer Properties of N-Butanol Extract of the Endemic Perralderia Coronopifolia”. Bangladesh Journal of Pharmacology, vol. 13, no. 1, Mar. 2018, pp. 82-89, doi:10.3329/bjp.v13i1.34255.

Issue

Vol. 13 No. 1 (2018)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).