Effect of sevoflurane postconditioning on myocardial oncosis and autophagy in ischemia-reperfusion injury in isolated rat heart
Keywords:Heart, Ischemia-reperfusion, Myocardial oncosis, Sevoflurane
The effects of sevoflurane postconditioning on myocardial oncosis and autophagy were studied in 32 isolated rat heart of ischemia-reperfusion injury. The hearts were perfused by: a) Sham surgery group, b) simple sevoflurane group, c) simple ischemia-reperfusion group (I/R) group, and d) sevoflurane postconditioning group. The ratios between the gray density values of the target bands to the gray density value of GADPH were used to reflect the Beclin-1, LC3II/I, and porimin expression levels. The LC3II/I level in the sevoflurane postconditioning group was lower than the level in the I/R group. The porimin level in the sevoflurane postconditioning group was lower than the level in the I/R group. The myocardial infarction range in the sevoflurane postconditioning group (33 ± 5%) was significantly diminished compared with the range in the I/R group (53 ± 6%) (p<0.05). Sevoflurane decreased the occurrence of oncosis and alleviated myocardial ischemia-reperfusion injury by inhibiting MPTP opening.
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