Hydrogen sulfide-mediated cardioprotection against ischemia reperfusion is linked to KATP channel for mitochondrial preservation but not for its distinct preference on interfibrillar mitochondria

Authors

  • Priyadharshini Chandrasekaran Vascular Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamilnadu 613401, India. https://orcid.org/0000-0003-0009-7802
  • Sriram Ravindran Vascular Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamilnadu 613401, India. https://orcid.org/0000-0003-4829-3626
  • Sri Rahavi Boovarahan Vascular Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamilnadu 613401, India. https://orcid.org/0000-0002-1465-7834
  • Gino A. Kurian Vascular Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamilnadu 613401, India.

DOI:

https://doi.org/10.3329/bjp.v14i2.39890

Keywords:

Hydrogen sulfide, Ischemia reperfusion; Ischemic preconditioning, Mitochondrial KATP channel, Diazoxide

Abstract

Hydrogen sulfide has been shown to protect  myocardium against ischemia-reperfusion injury by preserving interfibrillar mitochondria functional activities than subsarcolemmal mitochondria. In this study, the role of the KATP channel in modulating the mitochondrial subpopulations during the cardioprotection mediated by NaSH (H2S donor) was investigated. Isolated rat hearts were treated with mitochondrial KATP channel closer glibenclamide (10 μM)/opener diazoxide (0.8 mM) via Langendorff perfusion apparatus before ischemia-reperfusion. The results showed that NaSH pre-conditioning in presence of glibenclamide significantly improved cardiac recovery without any significant difference between interfibrillar mitochondria and subsarcolemmal mitochondria.  In conclusion, targeting KATP channel may not be good option to target interfibrillar mitochondria/subsarcolemmal mitochondria against ischemia-reperfusion injury.

Video Clip of Methodology:

Langendorff isolated rat heart experiment: 4 min 44 sec:  Click to watch

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Author Biography

Gino A. Kurian, Vascular Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamilnadu 613401, India.

Senior Assistant Professor

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Published

2019-06-09

How to Cite

Chandrasekaran, P., S. Ravindran, S. R. Boovarahan, and G. A. Kurian. “Hydrogen Sulfide-Mediated Cardioprotection Against Ischemia Reperfusion Is Linked to KATP Channel for Mitochondrial Preservation But Not for Its Distinct Preference on Interfibrillar Mitochondria”. Bangladesh Journal of Pharmacology, vol. 14, no. 2, June 2019, pp. 107-15, doi:10.3329/bjp.v14i2.39890.

Issue

Vol. 14 No. 2 (2019)

Section

Research Articles

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