Hepatoprotective effect of Misopates orontium in rats
DOI:
https://doi.org/10.3329/bjp.v15i3.45334Keywords:
Antioxidant, DNA protection Assay, Hemolysis, Misopates orontiumAbstract
This study explores the hepatoprotective and in vivo antioxidant activities of Misopates orontium. Toxicity studies were conducted on human red blood cells and DNA. Initially, acute hepatoprotective study was done on Wistar rats by giving crude, aqueous, n-hexane, chloroform, ethyl acetate and n-butanol fractions 200 mg/kg/day for 24 hours. Liver function tests were performed. The ethyl acetate fraction was selected for 14 days study. Hepatotoxicity induced by paracetamol (350 mg/kg) orally. All the liver function tests, liver peroxidation tests i.e malondialdehyde, glutathione and histopathological study of the liver was performed at the end of study. In hepatoprotective studies, ethyl acetate fraction showed a significant decrease in the level of serum enzymes (97.6 IU/L), (101 IU/L), (145.17 IU/L) and (0.68 mg/dL). Peroxidation levels in liver homogenates of ethyl acetate-treated group decreased in malondialdehyde (42.5) while reduced glutathione level increased (106.5 nmol/g). This study shows that M. orontium has hepatoprotective and antioxidant effects.
Downloads
23
21
References
Abdessemed D, Fontanay S, Duval RE, Mattar DL, Dibi A. Two new anthraquinone glycosides from Gladiolus segetum. Arab J Sci Eng. 2011; 36: 57-62.
Ahsan R, Islam KM, Musaddik A, Haque E. Hepatoprotective activity of methanol extract of some medicinal plants against carbontetrachloride induced toxicity in albino rats. Global J Pharmacol. 2009; 3: 116-22.
Al-Snafi AE. The pharmacological importance of Antirrhinum majus: A review. Asian J Pharm Sci Tech. 2015; 5: 313-20.
Bhatt DR, Jethva KD, Zaveri MN. Cytotoxic activity of alcoholic extract and its fractions of Eulophia nuda tubers on MCF7 cell line. J Pharmacogn Phytochem. 2018; 7: 704-07.
Bahmani M, Shirzad H, Rafieian S, Rafieian-Kopaei M. Sily-bum marianum: Beyond hepatoprotection. J Evid Based Complement Altern Med. 2015; 20: 292-301.
Hegde K, Joshi AB. Hepatoprotective and antioxidant effect of Carissa spinarum root extract against CCI4-and paracetamol-induced hepatic damage in rats. Bangladesh J Pharmacol. 2010; 5: 73-6.
Hassan I, Keen A, Majid S, Hassan T. Evaluation of the antioxidant status in patients of lichen planus in Kashmir valley: A hospital based study. J Saudi Soc Dermatol Dermatol Surg. 2013; 17: 13-16.
Gnanadesigan M, Ravikumar S, Anand M. Hepatoprotective activity of Ceriops decandra (Griff.) Ding Hou mangrove plant against CCl4 induced liver damage. J. Taib Uni. Sci. 2017; 11: 450-57.
Jabeen A, Khan MA, Ahmad M, Zafar M, Ahmad F. Indigenous uses of economically important flora of Margallah hills national park, Islamabad, Pakistan. Afr J Biotechnol. 2009; 8: 763-84.
Jurkovič S, Osredkar J, Marc J. Molecular impact of glutathione peroxidases in antioxidant processes. Biochemia Medica. 2008; 18: 162-74.
Kanchana N, Sadiq AM. Hepatoprotective effect of Plumbago zeylanica on paracetamol-induced liver toxicity in rats. Int J Pharm Pham Sci. 2011; 3: 151-54.
Li S, Han Q, Qiao C, Song J, Cheng CL, Xu H. Chemical markers for the quality control of herbal medicines: An overview. Chin Med. 2008; 3: 1-18.
Lönnig WE, Stüber K, Saedler H, Kim JH. Biodiversity and Dollo's Law: To what extent can the phenotypic differences between Misopates orontium and Antirrhinum majus be bridged by mutagenesis? Biorem Biodiv Bioavail. 2007; 1: 1-30.
Mallory HT, Evelyn KA. The determination of bilirubin with photoelectric calorimeter. J Biol Chem. 1937; 119: 481-90.
Merghem M, Dahamna S, Khennouf S. In vivo antioxidant activity of Ruta montana L. extracts. J Mater Environ Sci. 2019; 10: 470-77.
Mumtaz SF, Bag AK. Hepatoprotective and nephroprotective activity of hydroalcoholic extract of Ipomoea staphylina leaves. Bangladesh J Pharmacol. 2013; 8: 263-8.
Nadeem MPC, Dandiya PC, Pasha M, Imran D, Balani K, Vohora SB. Hepatoprotective activity of Solanum nigrum fruits. Fitoterapia 1997; 68: 245-51.
Rehman JU, Aktar N, Khan MY, Ahmad K, Ahmad M, Sultana S, Asif HM. Phytochemical screening and hepatoprotective effect of Alhagi maurorum Boiss (Leguminosae) against paracetamol-induced hepatotoxicity in rabbits. Trop J Pharm Res. 2015; 14: 1029-34.
Riaz M, Rasool N, Rasool S, Rashid U, Bukhari IH, Zubair M, Noreen M, Abbas M. Chemical analysis, cytotoxicity and antimicrobial studies by snapdragon: A medicinal plant. Asian J. Chem. 2013; 25 :5479.
Saleem M, Asif A, Akhtar MF, Saleem A. Hepatoprotective potential and chemical characterization of Artocarpus lakoocha fruit extract. Bangladesh J Pharmacol. 2018; 13: 90-2018.
Shenoy KA, Somayaji SN, Bairy KL. Evaluation of hepatoprotective activity of Ginkgo biloba in rats. Indian J Physiol Pharmacol. 2002; 46: 167‑74.
Sisein EA. Biochemistry of free radicals and antioxidants. Sch Acad J Biosci. 2014; 2: 110-18.
Srinivasan R, Chandrasekar MJN, Nanjan MJ, Suresh B. Antioxidant activity of Caesalpinia digyna root. J Ethnopharmacol. 2007; 113: 284-91.
Sumantha M, Ahmed R. Antihepatotoxic and antioxidant activity of root of Taraxacum officinale in CCl4 intoxicated rats. Pharmacogn Mag. 2008; 4: 188-94.
Tapadiya GG, Saboo SS, Farooqui IA, Khadabadi SS. Free radical scavenging, in vivo antioxidant and hepatoprotective activity of folk medicine Trichodesma sedgwickianum. Bangladesh J Pharmacol. 2013; 8: 58-64.
Uddin SJ, Grice ID, Tiralongo E. Cytotoxic effects of Bangladeshi medicinal plant extracts. Evid Based Complement Altern Med. 2011; 1-7.
Wan TJ, Shen SM, Hwang HY, Fang HY. Soybean oil biodegradation using Pseudomonas aeruginosa attached on porous ceramic or polyurethane. Arab J Sci Eng. 2013; 38: 1025-30.
Wang D, Zhao Y, Jiao Y, Yu L, Yang S, Yang X. Antioxidative and hepatoprotective effects of the polysaccharides from Zizyphus jujube cv. Shaanbeitanzao. Carbohydr Polym. 2012; 88: 1453-59.
Weng CJ, Chen MJ, Yeh CT, Yen GC. Hepatoprotection of quercetin against oxidative stress by induction of metallothionein expression through activating MAPK and PI3K pathways and enhancing Nrf2 DNA-binding activity. New Biotechnol. 2011; 28: 767-77.
Weng XC, Gordon MH. Antioxidant activity of quinones extracted from tanshen (Salvia miltiorrhiza Bunge). J Agric Food Chem. 1992; 40: 1331-36.
Zhou G, Chen Y, Liu S, Yao X, Wang Y. In vitro and in vivo hepatoprotective and antioxidant activity of ethanolic extract from Meconopsis integrifolia (Maxim.) Franch. J Ethnopharmacol. 2013; 148: 664-70.
Zhou DN, Ruan JL, Cai YL, Xiong ZM, Fu W, Wei AH. Antioxidant and hepatoprotective activity of ethanol extract of Arachniodes exilis (Hance) Ching. J Ethnopharmacol. 2010; 129: 232-37.
Zubair M, Rizwan K, Rashid U, Saeed R, Saeed AA, Rasool N, Riaz M. GC/MS profiling, in vitro antioxidant, antimicrobial and haemolytic activities of Smilax macrophylla leaves. Arab J Chem. 2017; 10: S1460-68.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
