Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat

Authors

  • Beauty Saha Department of Pharmacology, Bangabnadhu Sheikh Mujib Medical University, Shahbag, Dhaka

DOI:

https://doi.org/10.3329/bjp.v1i2.491

Keywords:

Arsenic, Ascorbic acid, Gluthathione, Liver, Rat

Abstract

Isolated liver tissues of rat were loaded with trivalent arsenic and were exposed in presence and absence of ascorbic acid. The amount of reduced glutathione (GSH) in normal liver tissue was 52.0 ± 0.2 ?g/g protein. Addition of arsenic to the tissues reduced the amount of GSH to 11.5 ± 0.3 ?g/g protein. But when the arsenic loaded liver tissues were incubated with ascorbic acid at the concentration of 20 ?g/ml, the amount of GSH was 14.2 ± 0.1 ?g/g protein. There was 22.6% increase of GSH level which was statistically significant (p<0.001) when compared with arsenic alone. This study suggests that ascorbic acid increased the GSH level in arsenic-treated rat's liver.

Downloads

Download data is not yet available.
Abstract
225
Download
102 Read
2

References

Chattopadhyay S, Bhaumik S, Purkayastha M, Basu S, Chaudhuri AN, Gupta SD. Apoptosis and necrosis in developing brain cells due to arsenic toxicity and protection with antioxidants. Toxicol Lett. 2002; 136: 65-76.

Meister A. Glutathione-ascorbic acid antioxidant system in animals. J Biol Chem. 1994; 269: 9397-00.

Patrick L. Toxic metals and antioxidants. Altern Med Rev. 2003; 8: 106-28.

Rabbani GH, Saha SK, Marni F, Akhtar M, Alauddin M, Mitra AK, Nasir M, Chowdhury AKA. Antioxidants in detoxification of arsenic-induced oxidative injury in rabbits. Bangladesh arsenic control society, Dhaka, 2002, pp 69-77.

Shi H, Shi X, Liu KJ. Oxidative mechanism of arsenic toxicity and carcinogenesis. Mol Cell Biochem. 2004; 255: 67-78.

Styblo M, Thomas DJ. In vitro inhibition of glutathione reductase by arsenotriglutathione. Biochem Pharmacol. 1995; 49: 971-77.

Downloads

Additional Files

How to Cite

Saha, B. “Effect of Ascorbic Acid on Reduced Glutathione Level in Arsenic-Loaded Isolated Liver Tissues of Rat”. Bangladesh Journal of Pharmacology, vol. 1, no. 2, Dec. 2006, pp. 68-71, doi:10.3329/bjp.v1i2.491.

Issue

Vol. 1 No. 2 (2006)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).