Effect of Abutilon indicum in thyroxine-induced hyperthyroidism in rat
DOI:
https://doi.org/10.3329/bjp.v16i3.54174Keywords:
Abutilon indicum , Hyperthyroidism, ThyroxineAbstract
The study was designed to investigate the antithyroid activity of the crude methanolic (70%) extract of aerial parts of Abutilon indicum in male albino rats. The extract was prepared and analyzed for the presence of phytochemical constituents through preliminary chemical analysis, antioxidant assay and GC-MS. The in vivo antithyroid activities in thyroxine-induced hyperthyroidism were studied. Phytochemical analysis showed the presence of alkaloids, flavonoids and phenols, also verified by the data obtained from GC-MS. Thyroxine increased the levels of triiodothyronine (4.9 ± 0.1 ng/mL) and total thyroxine (9.4 ± 0.2 μg/dL); while, A. indicum at the doses of 300 and 500 mg/kg, showed significant decrease in the elevated levels of triiodothyronine (3.0 ± 0.1 and 2.6 ± 0.2 ng/mL) and thyroxine (7.7 ± 0.2 and 7.1 ± 0.2 μg/dL), respectively. Histopathological studies showed the restoration of filled follicular colloids in extract-treated animals. The results show that A. indicum exhibits dose-dependent antithyroid effects.
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Abdul M, Sarker AA, Saiful IM, Muniruddin A. Cytotoxic and antimicrobial activity of the crude extract of Abutilon indicum. Int J Pharmacogn Phytochem Res. 2010; 2: 1-4.
Ahmed D, Dar P, Chaudhery R, Masih R. Chemical constituents of Lagenaria siceraria mesocarp and its xanthine oxidase and alpha-amylase inhibitory activities. Int J Fruit Sci. 2017; 17: 310-22.
Akinmoladun AC, Ibukun E, Afor E, Akinrinlola B, Onibon T, Akinboboye A, Obuotor E, Farombi E. Chemical constituents and antioxidant activity of Alstonia boonei. Afr J Biotechnol. 2007; 6: 1197-201.
Azharuddin M, Atif M, Ahmed MI, Bakhtiary SA, Ibrahim M. Evaluation of antithyroid activity of Ficus racemosa Linn bark in male rats. Int J Pharm Pharm Sci. 2015; 7: 118-22.
Barbara TSY, Lindsey KL, Taylor MB, Erasmus DG, Jäger AK. The pharmacological screening of Pentanisia prunelloides and the isolation of the antibacterial compound palmitic acid. J Ethnopharmacol. 2002; 79: 101-07.
Birringer M, Pilawa S, Flohé L. Trends in selenium biochemis-try. Nat Prod Rep. 2002; 19: 693-18.
Chakraborty AK, Chandewar A, Charde MS. Phytochemical screening & in vivo fertility enhancing activity (aphrodisiac) of Abutilon indicum roots on male Wistar rats. Asian J Chem Sci. 2017; 2: 1-9.
Chandrashekhar V, Nagappa A, Channesh T, Habbu P, Rao K. Anti-diarrhoeal activity of Abutilon indicum Linn leaf extract. J Nat Remedies. 2004; 4: 12-16.
Chhabra D, Gupta RK. Fortification of curd using Delonix regia flower petal extract and estimation of its phytochemical, antibacterial & antioxidant activity. J Pharmacogn Phytochem. 2015; 4: 299-07.
Corvilain B, Sande JV, Laurent E, Dumont JE. The H2O2-generating system modulates protein iodination and the activity of the pentose phosphate pathway in dog thyroid. Endocrinology. 1991; 128: 779-85.
Dashputre N, Naikwade N. Immunomodulatory activity of Abutilon indicum Linn on albino mice. Int J Pharm Sci Res. 2010; 1: 178-84.
Dashputre N, Naikwade N. Evaluation of anti-ulcer activity of methanolic extract of Abutilon indicum Linn leaves in experimental rats. Int J Pharm Sci Res. 2011; 3: 97-100.
Edupuganti S, Gajula RG, Kagitha CS, Kazmi N. Antimicrobial activity of Abutilon indicum. World J Pharm Pharm Sci. 2015; 4: 946-49.
Evans CR, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997; 2: 152-59.
Gomathi D, Kalaiselvi M, Ravikumar G, Devaki K, Uma C. GC-MS analysis of bioactive compounds from the whole plant ethanolic extract of Evolvulus alsinoides (L.). J Food Sci Technol. 2015; 52: 1212-17.
Hall JE, Guyton AC. Guyton and Hall: Textbook of medical physiology. 2017.
Hamdi A, Majouli K, Abdelhamid A, Marzouk B, Belghith H, Chraief I, Bouraoui A, Marzouk Z, Heyden YV. Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves. J Ethnopharmacol. 2018; 216: 97-103.
Harada H, Yamashita U, Kurihara H, Fukushi E, Kawabata J, Kamei Y. Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 2002; 22: 2587-90.
Hayat MM, Sarwar S, Anjum S, Uzair M, Rasheed HMF, Jabeen Q, Choudhary BA, Ashraf M. Anti-diabetic and spasmolytic potential of Farsetia hamiltonii Royle from Cholistan desert. J Ethnopharmacol. 2014; 156: 347-52.
Imo C, Uhegbu FO. Phytochemical analysis of Gongronema latifolium Benth leaf using gas chromatographic flame ionization detector. Int J Chem Biol Sci. 2015; 1: 60-68.
Javed F, Jabeen Q, Aslam N, Awan AM. Pharmacological evaluation of analgesic, anti-inflammatory and antipyretic activities of ethanolic extract of Indigofera argentea Burm F. J Ethnopharmacol. 2020; 259: 112966.
Jeong JB, Hong SC, Jeong HJ, Koo JS. Anti-inflammatory effect of 2-methoxy-4-vinylphenol via the suppression of NF-κB and MAPK activation, and acetylation of histone H3. Arch Pharm Res. 2011; 34: 2109-16.
Kailasam KV. Abutilon indicum L (Malvaceae)-medicinal potential review. Phcog J. 2015; 7: 330-32.
Kalyani B. Hepatoprotective and antioxidant role of flower extract of Abutilon indicum. Int J Curr Pharm Res. 2011; 2: 541-45.
Katzung BG, Trevor AJ. Basic and clinical pharmacology. New York, McGraw-Hill, 2015.
Kohn LD, Suzuki K, Nakazato M, Royaux I, Green ED. Effects of thyroglobulin and pendrin on iodide flux through the thyrocyte. Trends Endocrinol Metab. 2001; 12: 10-16.
Krisanapun C, Lee SH, Peungvicha P, Temsiririrkkul R, Baek SJ. Antidiabetic activities of Abutilon indicum (L.) Sweet are mediated by enhancement of adipocyte differentiation and activation of the GLUT1 promoter. Evid Based Complement Altern Med. 2011; 2011.
Kumarappan CT, Thilagam E, Mandal SC. Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens. Saudi J Biol Sci. 2012; 19: 349-55.
Kuo PC, Yang ML, Wu PL, Shih HN, Thang TD, Dung NX, Wu TS. Chemical constituents from Abutilon indicum. J Asian Nat Prod Res. 2008; 10: 689-93.
Lim TK. Abutilon indicum. Edible medicinal and non-medicinal plants. Springer, 2014. pp 276-89.
Malik BA, Butt MA. Is delayed diagnosis of hypothyroidism still a problem in Faisalabad, Pakistan. J Pakistan Med Assoc. 2008; 58: 545-49.
Manna D, Roy G, Mugesh G. Antithyroid drugs and their analogues: Synthesis, structure, and mechanism of action. Acc Chem Res. 2013; 46: 2706-15.
Meier B, Berger D, Hoberg E, Sticher O, Schaffner W. Pharmacological activities of Vitex agnus-castus extracts in vitro. Phytomedicine 2000; 7: 373-81.
Mono T, Shinohara R, Iwase K, Kotake M, Hamada M, Uchimura K, Hayakawa N, Hayashi R, Nakai A, Ishizuki Y. Changes in free radical scavengers and lipid peroxide in thyroid glands of various thyroid disorders. Horm Metab Res. 1997; 29: 351-54.
Panda S, Kar A. Annona squamosa seed extract in the regulation of hyperthyroidism and lipid-peroxidation in mice: Possible involvement of quercetin. Phytomedicine 2007; 14: 799-805.
Paranjape AN, Mehta AA. Investigation into the mechanism of action of Abutilon indicum in the treatment of bronchial asthma. Glob J Pharmacol. 2008; 2: 23-30.
Prachi CN, Kumar D, Kasana M. Medicinal plants of Muzaffarnagar district used in treatment of urinary tract and kidney stones. Indian J Tradit Knowl. 2009; 8: 191-95.
Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K. Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res. 2008; 102: 981-88.
Rajab NMA, Ukropina M, Milosevic MC. Histological and ultrastructural alterations of rat thyroid gland after short-term treatment with high doses of thyroid hormones. Saudi J Biol Sci. 2017; 24: 1117-25.
Rasheed HMF, Rasheed F, Qureshi AW, Jabeen Q. Immunostimulant activities of the aqueous methanolic extract of Leptadenia pyrotechnica, a plant from Cholistan desert. J Ethnopharmacol. 2016; 186: 244-50.
Reid JR, Wheeler SF. Hyperthyroidism: Diagnosis and treatment. Am Fam Physician. 2005; 72: 623-30.
Shah MA, Khan RA, Ahmed M. Phytochemical analysis, cytotoxic, antioxidant and anti-diabetic activities of the aerial parts of Sorghum halepense. Bangladesh J Pharmacol. 2019; 14: 144-51.
Singhai AK. Abutilons indicum Linn: A phytopharmacological review. Int J Pharm Chem. 2013; 3: 2231-81.
Soobrattee MA, Neergheen VS, Ramma AL, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutat Res. 2005; 579: 200-13.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Int Pharm Sci. 2011; 1: 98-106.
Tripathi P, Chauhan N, Patel J. Anti-inflammatory activity of Abutilon indicum extract. Nat Prod Res. 2012; 26: 1659-61.
Truter I. Clinical review: Hyper- and hypothyroidism: Evidence-based pharmacy practice. SA Pharm J. 2011; 78: 10-14.
Vallabh D, Jadhav V, Kadam V. In-vitro anti-arthritic activity of Abutilon indicum (Linn.) Sweet. J Pharm Res. 2009; 2: 644-45.
Vassev KK, Olczyk K, Kucharz EJ, Marcisz C, Szczotka KW, Kotulska A. Free radical activity and antioxidant defense mechanisms in patients with hyperthyroidism due to Graves’ disease during therapy. Clin Chim Acta. 2000; 300: 107-17.
Vrca VB, Skreb F, Cepelak I, Mayer L. Supplementation with antioxidants in the treatment of Graves' disease: The effect on the extracellular antioxidative parameters. Acta Pharm. 2004; 54: 79-89.
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