Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells

Authors

  • Xia Jiang Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China. https://orcid.org/0000-0002-4282-7984
  • Jie Xie Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China. https://orcid.org/0000-0003-2503-0487
  • Zhaotun Hu Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China. https://orcid.org/0000-0003-1100-1255
  • Xiaoliang Xiang Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China. https://orcid.org/0000-0001-9577-6191

DOI:

https://doi.org/10.3329/bjp.v17i4.57734

Keywords:

Apoptosis, Cell differentiation, Neuroblastoma, Neuro-2a, Poria cocos

Abstract

Poria cocos is a widely used ingredient in traditional Chinese medicine and dietary supplement. In this study, the effects of the ethanol extract of P. cocos on cell proliferation in Neuro-2a neuroblastoma cells and its possible mechanism of action are investigated. The extract inhibited the growth of Neuro-2a cells in a dose-dependent manner with an IC50 value of 34.6 μg/mL. To further elucidate the mechanism by which the extract acts, transcriptome analysis using high-throughput Illumina RNA-seq technology was performed. Transcriptome analysis revealed that the cell cycle as the potential primary target pathway of the extract in Neuro-2a cells. Flow cytometry analysis confirmed that the extract induced cell cycle arrest at G1 phase. Furthermore, the extract also induced apoptosis in Neuro-2a cells. Notably, it was found that Neuro-2a cells exhibited morphological features of differentiation when treated with the extract. This study suggests that P. cocos may be a potential candidate therapeutic agent in combating neuroblastoma.

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Published

2022-11-19

How to Cite

Jiang, X., J. Xie, Z. Hu, and X. Xiang. “Ethanol Extract of Poria Cocos Induces Apoptosis and Differentiation in Neuro-2a Neuroblastoma Cells”. Bangladesh Journal of Pharmacology, vol. 17, no. 4, Nov. 2022, pp. 105-13, doi:10.3329/bjp.v17i4.57734.

Issue

Vol. 17 No. 4 (2022)

Section

Research Articles

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Copyright (c) 2022 Xiaoliang Xiang, Xia Jiang, Jie Xie, Zhaotun Hu

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