Anti-inflammatory effect of gallic acid on HaCaT keratinocytes through the inhibition of MAPK-, NF-kB-, and Akt-dependent signaling pathways


  • Polly Ho-Ting Shiu Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China. https://orcid org/0000-0003-4164-8797
  • Chengwen Zheng Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China.
  • Panthakarn Rangsinth Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China.
  • Wen Wang Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China.
  • Jingjing Li Department of Rehabilitation Sciences, Faculty of Health and Social Sciences, Hong Kong Polytechnic University, Hong Kong SAR, China.
  • Renkai Li Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China.
  • George Pak-Heng Leung Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China.



Anti-inflammatory, Antioxidant, Chemokines, Gallic acid, Keratinocytes


The objectives of the present study were to evaluate the antioxidant and anti-inflammatory effects of gallic acid, a naturally occurring triphenolic compound, on HaCaT keratinocytes and study its mechanisms of action. The results showed that gallic acid at concentrations lower than 30 µM was non-toxic to HaCaT cells, reduced the intracellular level of reactive oxygen species, and suppressed the release of chemokines interleukin-8 and monocyte chemoattractant protein-1 in tumor necrosis factor-α- and interferon-γ-stimulated HaCaT keratinocytes. In addition, gallic acid reduced the total protein expression of NF-κB and inhibited the phosphorylation of ERK1/2, p38 MAPK, and Akt in stimulated HaCaT keratinocytes. In conclusion, our study revealed that gallic acid exhibited antioxidant and anti-inflammatory effects on keratinocytes, probably through the inhibition of MAPK-, NF-κB-, and Akt-dependent signaling pathways.


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How to Cite

Shiu, P. H.-T., C. Zheng, P. . Rangsinth, W. Wang, J. Li, R. Li, and G. P.-H. Leung. “Anti-Inflammatory Effect of Gallic Acid on HaCaT Keratinocytes through the Inhibition of MAPK-, NF-KB-, and Akt-Dependent Signaling Pathways”. Bangladesh Journal of Pharmacology, vol. 18, no. 1, Jan. 2023, pp. 24-32, doi:10.3329/bjp.v18i1.63052.



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