Anti-inflammatory effect of gallic acid on HaCaT keratinocytes through the inhibition of MAPK-, NF-kB-, and Akt-dependent signaling pathways
DOI:
https://doi.org/10.3329/bjp.v18i1.63052Keywords:
Anti-inflammatory, Antioxidant, Chemokines, Gallic acid, KeratinocytesAbstract
The objectives of the present study were to evaluate the antioxidant and anti-inflammatory effects of gallic acid, a naturally occurring triphenolic compound, on HaCaT keratinocytes and study its mechanisms of action. The results showed that gallic acid at concentrations lower than 30 µM was non-toxic to HaCaT cells, reduced the intracellular level of reactive oxygen species, and suppressed the release of chemokines interleukin-8 and monocyte chemoattractant protein-1 in tumor necrosis factor-α- and interferon-γ-stimulated HaCaT keratinocytes. In addition, gallic acid reduced the total protein expression of NF-κB and inhibited the phosphorylation of ERK1/2, p38 MAPK, and Akt in stimulated HaCaT keratinocytes. In conclusion, our study revealed that gallic acid exhibited antioxidant and anti-inflammatory effects on keratinocytes, probably through the inhibition of MAPK-, NF-κB-, and Akt-dependent signaling pathways.
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Copyright (c) 2023 Polly Ho-Ting Shiu, Chengwen Zheng, Panthakarn Rangsinth, Wen Wang, Jingjing Li, Renkai Li, George Pak-Heng Leung
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