Analgesic and anti-inflammatory effects of hydroalcoholic extract of Astragalus ibrahimianus

Authors

  • Abdelfatah Aitbaba Laboratory of Pharmacology, Neurobiology, Anthropobiology and Environment, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco. https://orcid.org/0000-0002-5650-3868
  • Zahra Sokar Laboratory of Pharmacology, Neurobiology, Anthropobiology and Environment, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco. https://orcid.org/0000-0002-8646-4660
  • Abderrahman Chait Laboratory of Pharmacology, Neurobiology, Anthropobiology and Environment, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco. https://orcid.org/0000-0002-5639-1931

DOI:

https://doi.org/10.3329/bjp.v18i2.65135

Keywords:

Analgesic, Anti-inflammatory, Antioxidant, Astragalus ibrahimianus, FRAP Antioxidant Assay

Abstract

This study aims to investigate the analgesic, anti-inflammatory, and antioxidant effects of the hydroalcoholic extract of Astragalus ibrahimianus, an endemic plant of Morocco. The analgesic effect was tested using the hot plate, writhing, and formalin tests. The anti-inflammatory activity was assessed by carrageenan and xylene-induced paw/ear edema respectively. The antioxidant activity was evaluated by three tests, namely DPPH, reducing power, and iron chelation. A. ibrahimianus treatment resulted in a significant reduction in carrageenan-induced paw edema and xylene-induced ear edema. Acute pretreatment with the extract reduced the number of abdominal cramps induced by acetic acid injection, increased paw withdrawal latency in the hot plate test, and suppressed both phases of the formalin test. The extract showed significant antioxidant activity regarding the standard molecules. In conclusion, A. ibrahimianus has analgesic, anti-inflammatory, and antioxidant properties.

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References

Albayrak S, Kaya O. Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Turk J Biochem. 2018; 43: 425-34.

Belfihadj K. Prise en soins de la douleur en EHPAD. NPG. 2018; 18 : 218‑22.

Bellakhdar J, Claisse R, Fleurentin J, Younos C. Repertory of standard herbal drugs in the Moroccan pharmacopoea. J Ethnopharmacol. 1991; 35: 123‑43.

Benmeddour Z, Mehinagic E, Le Meurlay D, Louaileche H. Phenolic composition and antioxidant capacities of ten Algerian date (Phoenix dactylifera L.) cultivars: A comparative study. J Funct Foods. 2013; 5: 346-54.

Bhukya B, Anreddy RNR, William CM, Gottumukkala KM. Analgesic and anti-inflammatory activities of leaf extract of Kydia calycina Roxb. Bangladesh J Pharmacol. 2009; 4: 101-04.

Butkutė, B, Dagilytė A, Benetis R, Padarauskas A, Cesevičienė J, Olšauskaitė V, Lemežienė N. Mineral and phytochemical profiles and antioxidant activity of herbal material from two temperate Astragalus species. Biomed Res. Int. 2018; 2018: 11.

Cao, Y, Li H. Harmony between humanity and nature : Natural vs. synthetic drug preference in Chinese atheists and Taoists. J Rel Health. 2022; 61: 2743‑52.

Ferrante C, Recinella L, Locatelli M, Guglielmi P, Secci D, Leporini L. Protective effects induced by microwave-assisted aqueous harpagophytum extract on rat cortex synaptosomes challenged with amyloid b-peptide. Phytother Res. 2017; 31: 1257-64.

Ferreira J, Floriani AE, Cechinel Filho V, Delle Monache F, Yunes RA, Calixto JB, Santos AR. Antinociceptive properties of the methanolic extract and two triterpenes isolated from Epidendrum mosenii stems (Orchidaceae). Life Sci. 2000; 66: 791-802.

Fulgenzi, A, Dell'Antonio G, Foglieni C, Cin ED, Ticozzi P, Franzone JS, Ferrero ME. Inhibition of chemokine expression in rat inflamed paws by systemic use of the antihyperalgesic oxidized ATP. BMC Immunol. 2005; 6: 1-11.

Hakim A, Ghufran A, Nasreen J. Evaluation of anti-inflammatory activity of the pods of Iklil-ul-Malik (Astragalus hamosus Linn.). Indian J Nat Prod Resour. 2010; 1: 34-37

Halliwell B. The wanderings of a free radical. Free Radic Biol Med. 2008; 46: 531-42.

Heimler D, Vignolini P, Dini MG, Romani A. Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. dry beans. J Agric Food Chem. 2005; 53: 3053-56.

Hunskaar S, Hole K. The formalin test in mice: Dissociation between inflammatory and non-inflammatory pain. Pain 1987; 30: 103-14.

Khan A, Khan RA, Ahmed M, Mushtaq N. In vitro antioxidant, antifungal and cytotoxic activity of methanolic extract of Calligonum polygonoides. Bangladesh J Pharmacol. 2015; 10: 316-20.

Kızıltaş H, Bingol Z, Goren A, Pınar S, Alwasel S, Gülçin İ. LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer. J Chem. Metrol. 2021; 15: 135-51.

Kondeva-Burdina M, Simeonova R, Shkondrov A, Krasteva I, Popov G, Manov V. Hepatoprotective and antioxidant effects of alcesefoliside from Astragalus monspessulanus. Braz J Pharm Sci. 2022; 58: e18902

Krasteva I, Platikanov S, Nikolov S, Kaloga M. Flavonoids from Astragalus hamosus. Nat Prod Res. 2007; 21: 392-95.

Liaqat H, Kim KJ, Park SY, Jung SK, Park SH, Lim S, Kim JY. Antioxidant effect of wheat germ extracts and their antilipidemic effect in palmitic acid-induced steatosis in HepG2 and 3T3-L1 cells. Foods 2021; 10: 1061.

Liyana-Pathirana CM, Shahidi F. Antioxidant and free radical scavenging activities of whole wheat and milling fractions. Food Chem. 2007; 101: 1151-57.

Mansouri A, Embarek G, Kokkalou E, Kefalas P. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chem. 2005; 89: 411-20.

Meriem BA, Rania B. Contribution to the study of adverse effects of self-medication. Thesis, The University Larbi Tebessi-Tebessa. 2022.

Nascimento GE, Hamm LA, Baggio CH, de Paula Werner MF, Iacomini M, Cordeiro LM. Structure of a galactoarabinoglu-curonoxylan from tamarillo (Solanum betaceum), a tropical exotic fruit, and its biological activity. Food Chem. 2013; 141: 510-16.

Naseri M, Mojab F, Khodadoost M, Kamalinejad M, Davati A, Choopani R, Emtiazy M. The study of anti-inflammatory activity of oil-based dill (Anethum graveolens L.) extract used topically in formalin-induced inflammation male rat paw. Iran J Pharm Res. 2012; 11: 1169.

OECD. Test guideline 425. Acute oral toxicity. Statistical Programme (AOT 425 StatPgm). Version: 1.0. 2001.

Oguejiofor UCJ, Abubakar K, Ugwah M O, Njan AA. Evaluation of the antinociceptive and anti-inflammatory effect of Caralluma dalzielii. J Ethnopharmacol. 2013; 150: 967-72.

Okolo CO, Johnson PB, Abdurahman EM, Abdu-Aguye I, Hussaini IM. Analgesic efect of Irvingia gabonensis stem bark extract. J. Ethnopharmacol. 1995; 45: 125-29.

Oyaizu M. Studies on products of browning reaction anti-oxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986; 44: 307-15.

Rácz I, Zimmer A. Animals models of nociception. In: Standards of mouse model phenotyping. Hrabé de Angelis M, Chambon P, Brown S (eds). Weinheim, Wiley-VCH, 2006, pp 221-35.

Randolph BC, Peters MA. Analgesic effectiveness of ketorolac compared to meperidine in the rat formalin test. Anesth Prog. 1997; 44: 11.

Ridtitid W, Sae-Wong C, Reanmongkol W, Wongnawa M. Antinociceptive activity of the methanolic extract of Kaemp-feria galanga Linn. in experimental animals. J Ethnopharmacol. 2008; 118: 225-30.

Salehi B, Carneiro JN, Rocha JE, Coutinho HD, Morais Braga MF, Sharifi‐Rad J, Semwal P, Painuli S, Moujir LM, de Zarate Machado V, Janakiram S. Astragalus species: Insights on its chemical composition toward pharmacological applications. Phytother Res. 2021; 35: 2445-76.

Sheik A, Kim K, Varaprasad GL, Lee H, Kim S, Kim E, Shin JY, Oh SY, Huh YS. The anti-cancerous activity of adaptogenic herb Astragalus membranaceus. Phytomedicine 2021; 91: 153698.

Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. Vol. 299. Academic Press, 1999, pp 152-78.

Tang X, Lin Z, Cai W, Chen N, Shen L. Anti-inflammatory effect of 3-acethylaconitine. Acta Pharmacol Sin. 1984; 5: 85-89.

Teyeb H, Houta O, Najjaa H, Lamari A, Neffati M, Douki W, Najjar MF. Biological and chemical study of Astragalus gombiformis. Z Naturforsch C. 2012; 67: 367-74.

Wang D, Zhuang Y, Tian Y, Thomas GN, Ying M, Tomlinson B. Study of the effects of total flavonoids of Astragalus on atherosclerosis formation and potential mechanisms. Oxid Med Cell Longev. 2012; 2012: 282383.

Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med. 1962; 111: 544-47.

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64: 555-59.

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Published

2023-06-07

How to Cite

Aitbaba, A., Z. Sokar, and A. Chait. “Analgesic and Anti-Inflammatory Effects of Hydroalcoholic Extract of Astragalus Ibrahimianus”. Bangladesh Journal of Pharmacology, vol. 18, no. 2, June 2023, pp. 41-48, doi:10.3329/bjp.v18i2.65135.

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Research Articles