Anticataract activity of Erythrina stricta against naphthalene-induced cataractogenesis in rats

Authors

  • Muthuswamy Umamaheswari Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641 044, Tamil Nadu
  • Kuppuswamy Asokkumar Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641 044, Tamil Nadu
  • Thirumalaiswamy Sivashanmugam Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641 044, Tamil Nadu
  • Varadharajan Subhadradevi Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641 044, Tamil Nadu
  • Michael Neethu Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641 044, Tamil Nadu

DOI:

https://doi.org/10.3329/bjp.v5i2.6582

Keywords:

antioxidant activity, cataract, Erythrina stricta, lipid peroxidation, naphthalene, opacity index

Abstract

The anticataract activity of Erythrina stricta leaves against naphthalene-induced cataractogenesis was studied in Wistar albino rats. The various fractions of the leaf extract were administered orally (200 mg/kg) simultaneously with naphthalene (1 g/kg) for 28 days. At the end of the experiment, levels of malondialdehyde, lipid hydroperoxides, carbonyl and sulfhydryl content, enzymatic and non-enzymatic antioxidants in lens homogenate were measured. Administration of naphthalene produced a mature cataract and an increase in the opacity index. There was a significant increase in lipid peroxidation and a decrease in antioxidant enzymes when compared to normal control. Ophthalmoscopic observation indicated that simultaneous administration of the fractions delayed the onset and maturation of cataract. All the fractions (except the residual fraction) prevented the peroxidative damage caused by naphthalene. The leaves of E. stricta protected the lens against naphthalene damage which may be due to its anti-oxidant activity.

Downloads

Download data is not yet available.
Abstract
239
Download
139 Read
2

Author Biography

Muthuswamy Umamaheswari, Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641 044, Tamil Nadu

Assistant Professor

References

Altomare E, Grattagliano I, Vendemaile G, Micelli-Ferrari T, Signorile A, Cardia L. Oxidative protein damage in human diabetic eye: evidence for retinal participation. Eur J Clin Invest. 1997; 27: 141-47.

Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys. 1959; 82: 70-77.

Fukushi S, Merola LO, Kinoshita JH. Altering the course of cataracts in diabetic rats. Invest Ophthalmol Vis Sci. 1980; 19: 313-15.

Gupta SK. Drug screening methods. 1st ed. New Delhi, Jaypee brothers, 2004, pp 333-46.

Kanski JJ. Textbook of clinical ophthalmology: A systematic approach. 3rd ed. Oxford University Press, New York, 2003, pp 163-68.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984; 21: 130-32.

Kirtikar KR, Basu RD. Indian medicinal plants. 2nd ed. International Book Distributors, Dehradun, 1981, pp 781-83.

Lobarzewski J, Ginalska G. Industrial use of soluble or immobilized plant peroxidases. Plant Perox News Lett. 1995; 6: 3-7.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-75. PMid:14907713

Niehaus WG Jr, Samuelsson B. Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem. 1968; 6: 126-30.

OECD. Acute oral toxicity-acute oral toxic class method. Guideline 423, adopted 17.12.2001. In: Thirteenth Addendum to the OECD Guidelines for the testing of chemicals. Paris: organization for economic co-operation and development; 2000.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70: 158-69.

Racker E. Glutathione reductase from bakers' yeast and beef liver. J Biol Chem. 1955; 217: 855-65.

Singh H, Chawla AS, Kapoor VK, Kumar N, Piatak DM, Nowicki W. Investigation of Erythrina Spp. IX. Chemical Constituents of Erythrina stricta Bark. J Nat Prod. 1981; 44: 526-29.

Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972; 47: 389-94.

Sippel TO. Changes in the water, protein and glutathione contents of the lens in the course of galactose cataract development in rats. Invest Opthalmol. 1966; 5: 568-75.

Van Heyningen R, Pirie A. The metabolism of naphthalene and its toxic effect on the eye. Biochem J. 1967; 102: 842-52.

Vani RR, Rawal UM. Glutathione and glutathione redox cycle in riboflavin supplemented cataractous rat lenses. Indian J Pharmacol. 1966; 28: 93-97.

Downloads

Additional Files

Published

2010-12-27

How to Cite

Umamaheswari, M., K. Asokkumar, T. Sivashanmugam, V. Subhadradevi, and M. Neethu. “Anticataract Activity of Erythrina Stricta Against Naphthalene-Induced Cataractogenesis in Rats”. Bangladesh Journal of Pharmacology, vol. 5, no. 2, Dec. 2010, pp. 77-81, doi:10.3329/bjp.v5i2.6582.

Issue

Vol. 5 No. 2 (2010)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).