Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum

Authors

  • Sugashini Settu Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore 14, India. https://orcid.org/0000-0002-1928-5291
  • Sathiavelu Arunachalam Department of Agriculture Microbiology, Vellore Institute of Technology School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 14, India. https://orcid.org/0000-0001-9139-8357

DOI:

https://doi.org/10.3329/bjp.v18i2.65836

Keywords:

Alpha-amylase, Anti-diabetic, Bryophyllum pinnatum, 3T3 cell line, Daldinia eschscholtzii, DPPH, Endophytic fungi, Phomopsis mangiferae, Yeast cell

Abstract

The aim of the present study was to isolate endophytic fungi and examine their anti-diabetic as well as antioxidant potential. Twenty-nine endophytic fungi were isolated from the stem and root parts of Bryophyllum pinnatum. Based on the presence of phytoconstituents, 16 endophytic isolates were tested for the inhibition of α-amylase enzyme and free radicals. Thirteen isolates showed significant inhibition of α-amylase enzyme (18.8 ± 0.8 to 62.5 ± 0.4%) and free radicals (15.3 ± 0.2 to 87.4 ± 0.8%). Good inhibitions were found in six isolates, which was further tested to validates its anti-diabetic activity by glucose uptake in yeast cell and glucose diffusion inhibition studies. The most active isolates were identified as Daldinia eschscholtzii (BPS17) and Phomopsis mangiferae (BPK9) by microscopic and molecular identification method. Identified fungal isolates were tested for glucose uptake using 3T3 cell lines (100 µg/mL) and the result showed effective glucose utilization. These finding provides evidence that D. eschscholtzii and P. mangiferae are potent endophytic fungi having anti-diabetic activity.

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Author Biographies

Sugashini Settu, Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore 14, India.

Research Scholar

Sathiavelu Arunachalam, Department of Agriculture Microbiology, Vellore Institute of Technology School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 14, India.

Professor

References

Ali IJ, Anyanwu OO, Chukwudulue UM, Chinaka CN, Ogbiko C, Awemu G, Ahomafor JE, Onyeloni SO, Okolo CC, Obidiegwu OC, Okoye FB. Pharmacological and phytochemical potentials of endophytic fungi from Nigerian medicinal plants: A review. GSC Biol Pharm Sci. 2023; 23: 20-28.

Aransiola EF, Daramola MO, Iwalewa EO, Seluwa AM, Olufowobi OO. Anti-diabetic effect of Bryophyllum pinnatum leaves. Int J Biotechnol Bioeng. 2014; 8: 89-93.

Bai Y, Yi P, Zhang S, Hu J, Pan H. Novel antioxidants and α-glycosidase and protein tyrosine phospha-tise 1B inhibitors from an endophytic fungus Penicillium Brefeldianum F4a. J Fungi. 2021; 7: 1-10.

Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang CM. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules. 2022; 27: 1326.

Belayneh YM, Birru EM. Antidiabetic activities of hydromethanolic leaf extract of Calpurnia aurea (Ait.) Benth. Subspecies aurea (Fabaceae) in mice. Evid Based Complement Altern Med. 2018; 2018

Bhardwaj A, Sharma D, Jadon N, Agrawal PK. Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus roxburghii. Arch Clin Microbiol. 2015; 6: 1-9.

Chutulo EC, Chalannavar RK. Daldinia eschscholtzii: An endophytic fungi isolated from Psidium guajava as an alternative source of bioactive secondary metabolites. Asian J Mycol. 2020; 3: 376-98.

Daniel IE, Akpan EI, Utam EC. Phytochemical evaluation, antioxidant and antimicrobial activities of various extracts from leaves and stems of Bryophyllum pinnatum. Nepal J Biotechnol. 2020; 8: 17-28.

Das MS, Devi G. In vitro cytotoxicity and glucose uptake activity of fruits of Terminalia bellirica in vero, L-6 and 3T3 cell lines. J Appl Pharm Sci. 2015; 5: 92-95.

Effah-Yeboah E, Dartey E, Asare EA, Abraham JD, Kagya-Agyemang JK, Balali GI, Aboagye V. Effect of Kalanchoe crenata extract on renal and liver impairment, dyslipedemia and glycemia in streptozotocin induced diabetic rats. Annu Res Rev Biol. 2021; 36: 109-25.

Ezeagu CU, Elijah JP, Nwodo OF. Antidiabetic potential of ethanol leaf extract of Bryophyllum pinnatum on alloxan-induced diabetic rats and their haematological profiles. Afr J Pharm Pharmacol. 2017; 11: 526-33.

Ferdous KJ, Sohrab MH, Begum MN, Islam MR, Mazid MA. Morphological and molecular identification of endophytic fungi isolated from Zingiber officinale rocs. Bangladesh J Plant Taxon. 2022; 29: 361-71.

Fernandes JM, Cunha LM, Azevedo EP, Lourenço EM, Fernandes-Pedrosa MF, Zucolotto SM. Kalanchoe laciniata and Bryophyllum pinnatum: An updated review about ethnopharmacology, phytochemistry, pharmacology and toxicology. Braz J Pharm. 2019: 29: 529-58.

Gulyamova TG, Nasmetova SM, Ruzieva DM, Mukhammedov II, Kadyrova GK, Karimova FA. Phytochemical analysis of α-amylase inhibiting secondary metabolites of endophytic Penicillium brevicaule alba Thom. Eur J Mol Clin Med. 2020; 7: 830-36.

Gupta S, Adak S, Rajak RC, Banerjee R. In vitro efficacy of Bryophyllum pinnatum leaf extracts as potent therapeutics. Prep Biochem Biotechnol. 2015; 46: 489-94.

Gurnani N, Gupta M, Mehta D, Mehta BK. Chemical composition, total phenolic and flavonoid contents and in vitro antimicrobial and antioxidant activities of crude extracts from red chilli seeds (Capsicum frutescens L.). J Taibah Univ Sci. 2016; 10: 462-70.

Hamzah TN, Lee SY, Hidayat A, Terhem R, Faridah-Hanum I, Mohamed R. Diversity and characterization of endophytic fungi isolated from the tropical mangrove species, Rhizophora mucronata and identification of potential antagonists against the soil-borne fungus, Fusarium solani. Front Microbiol. 2018; 9: 1-17.

Jayamol MA, Ashley R, Nithya J, Shijina S. Effect of combination of aqueous leaf extracts of Psidium guajava Linn and Moringa oleifera Lam on diabetes mellitus. Int J Pharm Pharm Sci. 2020; 12: 79-82.

Jayant KK, Vijayakumar BS. In-vitro anti-oxidant and anti-diabetic potential of endophytic fungi associated with Ficus religiosa. Ital J Mycol. 2021; 50: 10-20.

Joseph B, Jini D. Antidiabetic effects of Momordica charantia (bitter melon) and its medicinal potency. Asian Pac J Trop Dis. 2013; 3: 93-102.

Kaur A. Evaluation of antidiabetic and antioxidant potential of endophytic fungi isolated from medicinal plants. Int J Green Pharm. 2018; 12: 1-14.

Kumar TS, Muthusamy P, Radha R, Ilango K. Formulation and evaluation of in vitro antidiabetic polyherbal tablets from some traditional used herbs. J Phytopharm. 2021; 10: 173-79.

Kumari M, Silva ID. Alpha-amylase inhibition activity of endophytic fungi isolated from leaves of Artocarpus heterophyllus. Int j Sci Res. 2018; 7: 731-34.

Mbachu KA, Ibok MG, Adeniyi-Akee MA, Ajala OE. Chemical compositions and antioxidant activity of leaf and stem essential oil of Bryophyllum pinnatum. GSC Biol Pharm Sci. 2019; 09: 057-64.

Mishra R, Kushveer JS, Khan MK, Sarma VV. Evaluation of antioxidant potential, DNA damage protection and anti-cancer activities of three endophytic fungi associated with selected medicinal plants. Int J Pharm Biol Sci. 2019; 9: 1174-84.

Naveen KV, Saravanakumar K, Sathiyaseelan A, Wang MH. Comparative analysis of the antioxidant, anti-diabetic, antibacterial, cytoprotective potential and metabolite profile of two endophytic Penicillium spp. Antioxidants 2023; 12: 1-22.

Ogbonnia SO, Odimegwu JI, Enwuru VN. Evaluation of hypoglycaemic and hypolipidaemic effects of aqueous ethanolic extracts of Treculia africana Decne and Bryophyllum pinnatum Lam. and their mixture on streptozotocin (STZ)-induced diabetic rats. Afr J Biotechnol. 2008; 7: 2535-39.

Ojewole JAO. Antinociceptive, anti-inflammatory and anti-diabetic effects of Bryophyllum pinnatum (Crassulaceae) leaf aqueous extract. J Ethnopharmacol. 2005: 99: 13-19.

Ojo OA, Ojo AB, Ajiboye BO, Olaiya O, Akawa A, Olaoye O, Anifowose OO, Idowu O, Olasehinde O, Obafemi T, Awe J. Inhibitory effect of Bryophyllum pinnatum (Lam.) Oken leaf extract and their fractions on α-amylase, α-glucosidase and cholinesterase enzyme. Pharmacogn J. 2018; 10: 497-506.

Okonkwo-Uzor NJ, Okezie UM, Eze PM, Ikegbune C, Okoye FB, Esimone CO. In vitro antimicrobial activity of secondary metabolites of an endophytic Aspergillus brunneoviolaceus isolated from Bryophyllum pinnatum (Lam.) Oken. J Clin Basic Res. 2022; 2: 249-54.

Paul S, Majumdar M. In vitro antidiabetic propensities, phytochemical analysis and mechanism of action of commercial antidiabetic polyherbal formulation Mehon. Proceedings. 2021; 79: 1-8.

Pavithra N, Sathish L, Babu N, Venkatarathanamma V, Pushpalatha H, Reddy GB, Ananda K. Evaluation of α-amylase, α-glucosidase and aldose reductase inhibitors in ethyl acetate extracts of endophytic fungi isolated from anti-diabetic medicinal plants. Int J Pharm Sci Res. 2014; 5: 5334-41.

Pottathil S, Nain P, Morsy MA, Kaur J, Al-Dhubiab BE, Jaiswal S, Nair AB. Mechanisms of antidiabetic activity of methanolic extract of Punica granatum leaves in nicotinamide/streptozotocin-induced type-2 diabetes in rats. Plants 2020; 9: 1-15.

Rustamova N, Gao Y, Zhang Y, Yili A. Biological activity of endophytic fungi from the roots of the medicinal plant Vernonia anthelmintica. Microorganisms 2020; 8: 586.

Sagbo IJ, van de Venter M, Koekemoer T, Bradley G. In vitro antidiabetic activity and mechanism of action of Brachylaena elliptica (Thunb.) DC. Evid Based Complement Altern Med. 2018; 2018.

Shah MAR, Khan RA, Ahmed M. Anti-diabetic activity of Iphiona aucheri leaf extract. Bangladesh J Pharmacol. 2020; 15: 99-109.

Shah MAR, Rahmat AK, Mushtaq A. Phytochemical analysis, cytotoxic, antioxidant and anti-diabetic activities of the aerial parts of Sorghum halepense. Bangladesh J Pharmacol. 2019; 14: 144-51.

Shankar S, Settu S, Segaran G, Sundar RD, Ravi L. Phytoche-mical constituents of Dracaena mahatma leaves and their antibacterial, anti-oxidant and anti-inflammatory significance. Biotechnol Res Innov. 2018; 2: 1-8.

Shettar AK, Vedamurthy AB. Evaluation of anti-diabetic potential of Kandelia candel and Rhizophora apiculta: An in vitro approach. Int J Pharm Sci Res. 2017; 8: 2551-59.

Shoba S, Sathiavelu M. Biological activities of endophytic fungus Cochliobolus sp.AL24 isolated from Aerva lanata L. Indian J Pharm Educ Res. 2018; 52: 277-83.

Singh B, Kaur A. Antidiabetic potential of a peptide isolated endophytic fungi Aspergillus awamori. J Appl Microbiol. 2016; 120: 301-11.

Sofa F, Akhmad D, Megawati M, Muhammad H. Isolation and identification of quercetin derivatives and their α-glucosidase inhibitory activities from Bryophyllum pinnatum. Res J Chem Environ. 2018; 22: 114-19.

Sowemimo AA, Edrada-Ebel R, Ebel R, Proksch P, Omobu-wajo OR, Adesanya SA. Major constituents of the predomi-nant endophytic fungi from the Nigerian plants Bryophyllum pinnatum, Morinda lucida and Jathropha gossypiifolia. Nat Prod Commu. 2008; 3: 1934578X0800300802.

Tejaswini HK, Jayashankar M, Saeed MA. Screening of biological effectiveness of endophytic fungi associated with Plectranthus amboinicus: A focus on Aspergillus terreus. Int J Pharm Sci Rev Res. 2021; 69: 24-31.

Tiwari P, Nathiya R, Mahalingam G. Antidiabetic activity of endophytic fungi isolated from Ficus religiosa. Asian J Pharm Clin Res. 2017; 10: 59-61.

Venkateswarulu N, Shameer S, Bramhachari PV, Thaslimbasha Sk, Nagaraju C, Vijaya T. Isolation and characterization of plumbagin (5-hydroxyl-2-methylnaptalene-1,4dione) producing endophytic fungi Cladosporium delicatulum from endemic medicinal plants. Biotechnol Rep. 2018; 20.

Vidyasagar GM, Naaz SQ, Ahmed LMD, Babanagre S, Sangeeta MK, Ambika V. Penicillium citrinum Thom: A potential antihyperglycemic endophyte from Gymnema sylvestre. Indian J Nat Prod Resour. 2021; 12; 557-63.

White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. 1990, pp 315-22.

Wutthiwong N, Suthiphasilp V, Pintatum A, Suwannarach N, Kumla J, Lumyong S, Maneerat T, Charoensup R, Cheenpracha S, Limtharakul T, Pyne SG. Daldiniaeschsone A, a rare tricyclic polyketide having a chromone unit fused to a δ-lactone and its symmetrical biphenyl dimer, daldiniaeschsone B, from an endophytic fungus Daldinia eschscholtzii SDBR-CMUNKC745. J Fungi. 2021; 7: 1-12.

Xiao F, Xu T, Lu B, Liu R. Guidelines for antioxidant assays for food components. Food Frontiers. 2020; 1: 60-69.

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Published

2023-06-07

How to Cite

Settu, S., and S. Arunachalam. “Anti-Diabetic Effect of Daldinia Eschscholtzii and Phomopsis Mangiferae Isolated from Bryophyllum Pinnatum”. Bangladesh Journal of Pharmacology, vol. 18, no. 2, June 2023, pp. 58-71, doi:10.3329/bjp.v18i2.65836.

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Research Articles