Effect of Cuminum cyminum and Rhus pentaphylla extracts on status epilepticus induced by intrahippocampal injection of kainic acid in rat


  • Khadija Oubella Laboratory of Pharmacology, Neurobiology, Anthropology and Environment, Department of Biology, Faculty of Sciences Semlalia, University Cadi Ayyad, BP 2390 40080 Marrakech, Morocco.
  • Karima Benrazzouk Laboratory of Agri-Food, Biotechnology, and Valorization of Plant Resources; Phytochemistry and Pharmacology of Medicinal Plants Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco.
  • Abdellah Bagri Laboratory of Biochemistry and Neuroscience, Integrative and Computational Neuroscience Team, Faculty of Sciences and Technology, Hassan First University, Settat 26002, Morocco.
  • Abderrahman Chait Laboratory of Pharmacology, Neurobiology, Anthropology and Environment, Department of Biology, Faculty of Sciences Semlalia, University Cadi Ayyad, BP 2390 40080 Marrakech, Morocco. https://orcid.org/0000-0002-5639-1931




Anticonvulsant, Antioxidant, Epilepsy, Kainic acid


This study aims to evaluate the anticonvulsant effect of the aqueous extract of Rhus pentaphylla and Cuminum cyminum (300 mg/kg), orally, 45 min before intrahippocampal injection of kainic acid (1 µg/µL). The scoring of seizure severity, latency of seizures and duration of total seizures were recorded in 90 min. Both extracts showed the presence of polyphenols, flavonoids, alkaloids and tannins compounds. These extracts exhibited potent antioxidant activity and showed an attenuation of severe convulsive seizures, a significant decrease in the frequency and duration of seizures and prolonged the latency of the onset seizure (p<0.001) compared to kainic acid. The aqueous extracts of R. pentaphylla and C. cyminum have a protective effect against seizures induced by kainic acid.


Download data is not yet available.


Adkar P, Jadhavn P, Ambavade S, Shelke T, Bhaskar V. Protective effect of leaf extract of Pandanus odoratissimus Linn on experimental model of epilepsy. Int J Nutr Pharmacol. 2014; 4: 81-87.

Aitbaba A, Sokar Z, Chait A. Analgesic and anti-inflammatory effects of hydroalcoholic extract of Astragalus ibrahimianus. Bangladesh J Pharmacol. 2023; 18: 41-48.

Ait Laaradia M, Oufquir S, El Hidan MA, Marhoume F, Laadraoui J, Bezza K, El Gabbas Z, Aboufatima R, Boumezzough A, Chait A. Assessment of the relationship between the amount of scorpion venom in the central nervous system and the severity of scorpion envenomation in rats. Toxin Rev. 2021; 40: 179-88.

Al-snafi AE. The pharmacological activities of Cuminum cyminum: A review. J Pharm. 2016; 6: 46-65.

Ambrogini P, Minelli A, Galati C, Betti M, Lattanzi D, Ciffolilli S, Piroddi M, Galli F, Cuppini R. Post-seizure α-tocopherol treatment decreases neuroinflammation and neuronal degeneration induced by status epilepticus in rat hippocampus. Mol Neurobiol. 2014; 50: 246-56.

Banso A, Adeyemo S. Phytochemical screening and antimalarial assessment of Abutilon mauritianum, Bacopa monni-fera and Datura stramonium. Biokemistri 2006; 18: 39-44.

Bezza K, El Gabbas Z, Laadraoui J, Laaradia MA, Oufquir S, Chait A. Ameliorative potential of Anacyclus pyrethrum extract in generalized seizures in rat: Possible cholinergic mediated mechanism. Bangladesh J Pharmacol. 2019; 14: 188-195.

Brunetti C, DI Ferdinando M, Fini A, Pollastri S, & Tattini M. Flavonoids as antioxidants and developmental regulators: Relative significance in plants and humans. Int J Mol Sci. 2013; 14: 3540-55.

Chen B, Choi H, Hirsch LJ, Katz A, Legge A, Buchsbaum R, Detyniecki K. Psychiatric and behavioral side effects of antiepileptic drugs in adults with epilepsy. Epilepsy Behav. 2017; 76: 24-31.

Choudhary N, Bijjem KR, Kalia AN. Antiepileptic potential of flavonoids fraction from the leaves of Anisomeles malabarica. J Ethnopharmacol. 2011; 135: 238-42.

Chouhan H, Purohit A, Ram H, Chowdhury S, Kashyap P, Panwar A, Kumar A. The interaction capabilities of phytoconstituents of ethanolic seed extract of cumin (Cuminum cyminum L.) with HMG‐CoA reductase to subside the hypercholesterolemia: A mechanistic approach. Food Frontiers. 2022; 3: 300-15.

Coyle JT. Kainic acid: Insights into excitatory mechanisms causing selective neuronal degeneration. In: Ciba Foundation Symposium 126‐Selective Neuronal Death: Selective neuronal death: Ciba Foundation Symposium. 2007; 126: 186-203.

Daneshkhah M, Setorki M. Effect of Artemisia persica on seizure severity and memory and learning disorders in pentylenetetrazole-kindled mice. Bangladesh J Pharmacol. 2019; 14: 36-44.

Diniz TC, Silva JC, Lima-Saraiva SRGD, Ribeiro FPRDA, Pacheco AGM, de Freitas RM, Almeida JRGDS. The role of flavonoids on oxidative stress in epilepsy. Oxid Med Cell Longev. 2015; 2015.

Dohou N, Yamni K, Tahrouch S, Massani LMI, Badoc A, Gmira N.. Screening phytochimique d’une endémique Libéro-Marocaine, Thymelaea luthroïdes. Bull Soc Pharm Bordx. 2003; 142: 61-78.

Fadhil H, Mraihi F, Zouied D, Ayadi MT, Cherif JK. Anticorrosion inhibition behavior of Rhus pentaphylla fruit extracts in (1 M) HCl against carbon steel and their chemical characterization using HPLC-MS-ESI. Chem Select. 2021; 6: 5281-89.

Ferr B, Gervasoni D, Vogt C. Précis de neurochirurgie stéréotaxique appliquée aux rongeurs de laboratoire. Paris, Lavoisier, 2012, pp 122-27.

Forman HJ, Zhang H. Targeting oxidative stress in disease: Promise and limitations of antioxidant therapy. Nat Rev Drug Discov. 2021; 20: 689-709.

Gersner R, Ekstein D, Dhamne SC, Schachter SC, Rotenberg A. Huperzine A prophylaxis against pentylenetetrazole-induced seizures in rats is associated with increased cortical inhibition. Epilepsy Res. 2015; 117: 97-103.

Golechha M, Chaudhry U, Bhatia J, Saluja D, Singh Arya D. Naringin protects against kainic acid-induced status epilepticus in rats: Evidence for an antioxidant, anti-inflammatory and neuroprotective intervention. Biol Pharm Bull. 2011; 34: 360-65.

Hosseinzadeh H, Ramezani M, Fadishei M, Basirat M. Anticonvulsant effects of Cuminium cyminum L. seeds extracts and essential oil in mice. J Med Plant. 2002; 1: 9-14.

Joshi A, Bhobe M, Saatarkar A. Phytochemical investigation of the roots of Grewia microcos Linn. J Chem Pharm Res. 2013; 5: 80-87.

Kabuto H, Yokoi I, and Mori A. Monoamine metabolites, iron induced seizures, and the anticonvulsant effect of tannins. Neurochem Res. 1992; 17: 585-90.

Khatibi A, Haghparast A, Shams J, Dianati E, Komaki A, Kamalinejad M. Effects of the fruit essential oil of Cuminum cyminum L. on the acquisition and expression of morphine-induced conditioned place preference in mice. Neurosci. Lett. 2008; 448: 94-98.

Mansour HB, Yatouji S, Mbarek S, Houas I, Delai A, Dridi D. Correlation between antibutyrylcholinesterasic and antioxidant activities of three aqueous extracts from Tunisian Rhus pentaphyllum. Ann Clin Microbiol Antimicrob. 2011; 10: 1-9.

Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Calvo D, Paladini AC. Overview– Flavonoids: A new family of benzodiazepine receptor ligands. Neurochem Res. 1997; 22: 419-25.

Nassiri-Asl M, Farivar TN, Abbasi E, Sadeghnia HR, Sheikhi M, Lotfizadeh MPB. Effects of rutin on oxidative stress in mice with kainic acid-induced sei-zure. J Integr Med. 2013; 11: 337-42.

Okpo SO, Fatokun F, Adeyemi OO. Analgesic and anti-inflammatory activity of Crinum glaucum aqueous extracts. J Ethnopharmacol. 2001; 78: 207-11.

Oyaizu M. Studies on products of browning reaction anti-oxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986; 44: 307-15.

Paxinos G, Watson C. The rat brain in stereotaxic coordinates. London. Elsevier, 2006.

Racine RJ. Modification of seizure activity by electrical stimulation: II. Motor seizure. Electroencephalogr Clin Neuro-physiol. 1972; 32: 281-94.

Reddy AJ, Dubey AK, Handu SS, Sharma P, Mediratta PK, Ahmed QM, Jain S. Anticonvulsant and antioxidant effects of Musa sapientum stem extract on acute and chronic experi-mental models of epilepsy. Pharmacog Res. 2018; 10: 49.

Sardari S, Amiri M, Rahimi H, Kamalinejad M, Narenjkar J, Sayyah M. Anticonvulsant effect of Cicer arietinum seed in animal models of epilepsy: Introduction of an active molecule with novel chemical structure. Iran Biomed J. 2015; 19: 45.

Shamsiev A, Park J, Olawuyi IF, Odey G, Lee W. Optimization of ultrasonic-assisted extraction of polyphenols and anti-oxidants from cumin (Cuminum cyminum L.). Korean J Food Preserv. 2021: 28: 510-21.

Talavéra S, Felgines C, Texier O, Besson C, Gil-Izquierdo A, Lamaison JL, Rémésy C. Anthocyanin metabolism in rats and their distribution to digestive area, kidney, and brain. J Agric Food Chem. 2005; 53: 3902-08.

Wu Y, Chen M, Jiang J. Mitochondrial dysfunction in neurodegenerative diseases and drug targets via apoptotic sig-naling. Mitochondrion 2019; 49: 35-45.

Yimer EM, Tuem KB, Karim A, Ur-Rehman N, Anwar F. Nigella sativa L. (black cumin): A promising natural remedy for wide range of illnesses. Evid Based Complement Altern Med. 2019; 2019.

Youdim KA, Shukitt-Hale B, Joseph JA. Serial Review: Flavo-noids and isoflavones (Phytoestrogens: Absorption, metabolism, and bioactivity). Free Radic Biol Med. 2004; 36: 1683-93.

Zadali R, Baghery M, Abbasi M, Yavari N, Miran M, Ebrahimi SN. Anticonvulsant activity of Iranian medicinal plants and molecular docking studies of isolated phytochemicals. S Afr J Bot. 2022; 149: 646-57.

Zhang XM, and Zhu J. Kainic acid-induced neurotoxicity: Targeting glial responses and glia-derived cytokines. Curr Neuropharmacol. 2011; 9: 388-98.




How to Cite

Oubella, K. ., K. . Benrazzouk, A. . Bagri, and A. Chait. “Effect of Cuminum Cyminum and Rhus Pentaphylla Extracts on Status Epilepticus Induced by Intrahippocampal Injection of Kainic Acid in Rat”. Bangladesh Journal of Pharmacology, vol. 18, no. 3, Aug. 2023, pp. 105-12, doi:10.3329/bjp.v18i3.67165.



Research Articles