Berberine triggers necroptosis of cervical H8 cells by activating receptor-interacting protein kinase 1

Authors

  • Mengting Wang Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China.
  • Huixian Chen Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0009-0001-7684-0888
  • Qian Wu Department of Critical Care Medicine, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0009-0002-8060-1393
  • Yan Huang Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0000-0003-4801-3444
  • Yuzhen Zhou Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0000-0002-6978-3432

DOI:

https://doi.org/10.3329/bjp.v19i1.70889

Keywords:

Berberine, cervical H8 Cells, Cervical intraepithelial neoplasia, Necroptosis, Receptor-interacting protein kinase 1, Western blot

Abstract

This study aims to investigate the effect of berberine on necroptosis in cervical H8 cells. CCK-8 assay was used to assess cell proliferation activity. The expression levels of necroptosis key markers Receptor-interacting protein kinase 1 (RIPK1), MLKL, p-MLKL, and apoptosis-related caspase-8, caspase-3 were analyzed through qRT-PCR, western blot, and immunofluorescence. Mechanistic studies were conducted using the RIPK1 kinase inhibitor necrostatin-1 (Nec-1). The results showed that berberine could inhibit H8 cell proliferation in a time- and dose-dependent manner (p<0.05). It increased the expression of RIPK1 and MLKL (p<0.05), while inhibiting the expression of caspase-8 and caspase-3 (p<0.05). Nec-1 inhibitor decreased the expression of RIPK1 and MLKL after the intervention of berberine (p<0.05). Therefore, berberine induces cervical H8 cell death through the activation of RIPK1-mediated necroptosis.

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Published

2024-03-14

How to Cite

Wang, M., H. Chen, Q. Wu, Y. . Huang, and Y. . Zhou. “Berberine Triggers Necroptosis of Cervical H8 Cells by Activating Receptor-Interacting Protein Kinase 1”. Bangladesh Journal of Pharmacology, vol. 19, no. 1, Mar. 2024, pp. 13-22, doi:10.3329/bjp.v19i1.70889.

Issue

Vol. 19 No. 1 (2024)

Section

Research Articles

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Copyright (c) 2024 Mengting Wang, Huixian Chen, Qian Wu, Yan Huang, Yuzhen Zhou

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