In vitro anti-cancer activity of Epipremnum aureum
DOI:
https://doi.org/10.3329/bjp.v19i1.71024Keywords:
Anti-cancer, Epipremnum aureum, MCF-7 cell line, MTT assayAbstract
In the present study, in vitro anti-cancer activity of aerial parts of Epipremnum aureum extracts was performed using the MCF-7 breast cancer cell line. Soxhlet method was used with different solvents for extract preparation. The amount of apoptosis in MCF-7 cells was assessed using flow cytometry for each of the extracts. The chloroform and ethanol extracts had a considerable cytotoxic effect with IC50 values of 32.9 and 45.8 μg/mL respectively, while the conventional medication 5-fluorouracil produced an IC50 value of 19.2 μg/mL. The microscopic examination of the chloroform and ethanol extracts of E. aureum revealed the presence of apoptotic bodies, nuclear fragmentation, and tiny nuclei with strong chromatin condensation. These results suggest that chloroform extract of E. aureum is more effective against breast cancer.
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Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020; 12: 1-10.
Akram M, Iqbal M, Daniyal M, Khan AU. Awareness and current knowledge of breast cancer. Biol Res. 2017; 50: 1-23.
Ali A, Banerjee S, Kamaal S, Usman M, Das N, Afzal M, Alarifi A, Sepay N, Roy P, Ahmad M. Ligand substituent effect on the cytotoxicity activity of two new copper (ii) complexes bearing 8-hydroxyquinoline derivatives: Validated by MTT assay and apoptosis in MCF-7 cancer cell line (human breast cancer). RSC Adv. 2021; 11: 14362-73.
Al-Oqail MM. Anticancer efficacies of Kramerial appacea extracts against human breast cancer cell line (MCF-7): Role of oxidative stress and ROS generation. Saudi Pharm J. 2021; 29: 244-51.
Chazotte B. Labeling nuclear DNA using DAPI. Cold Spring Harb Protoc. 2011; 80-82.
Eidet JR, Pasovic L, Maria R, Jackson CJ, Utheim TP. Objective assessment of changes in nuclear morphology and cell distribution following induction of apoptosis. Diagn Pathol. 2014; 9: 92-100.
Khang DT, Dieu HD, Dung NK, Giang BT, Thao TL, Tien LT, Men TT, Loi HV, Tuan NT, Ay NV, Thuy NP. Anticancer and antioxidant of chloroform extracts from medical plants in the Mekong Delta, Vietnam. Asian J Plant Sci. 2020; 19: 398-405.
Kumar R, Saneja A, Panda AK. An annexin V-FITC-propidium iodide-based method for detecting apoptosis in a non-small cell lung cancer cell line. Methods Mol Biol. 2021; 2279: 213-23.
Lin S, Ke Z, Liu K, Zhu S, Li Z, Yin H, Chen Z. Identification of DAPI-stained normal, inflammatory, and carcinoma hepatic cells based on hyperspectral microscopy. Biomed Opt Express. 2022; 13: 2082-90.
Lan TM, Sulaiman SF, Muhammad TS. Anticancer medicinal plant, Epipremnum pinnatum (L.) Engl. chloroform extracts elicited both apoptotic and non-apoptotic cell deaths in T-47D mammary carcinoma cells. Curr Appl Sci Tech. 2007; 7: 24-43.
Malla R, Surepalli N, Farran B, Malhotra SV, Nagaraju GP. Reactive oxygen species (ROS): Critical roles in breast tumor microenvironment. Cri Rev Oncol Hematol. 2021; 160: 103285-96.
Meshram A, Srivastava N. Phytochemical screening and in vitro antioxidant potential of methanolic extract of Epipremnum aureum (Linden and Andre) GS Bunting. Int J Pharm Res Allied Sci. 2016 1; 5: 1-6.
Nerdy N, Lestari P, Sinaga JP, Ginting S, Zebua NF, Mierza V, Bakri TK. Brine shrimp (Artemiasalina Leach.) lethality test of ethanolic extract from green betel (Piper betle Linn.) and red betel (Piper crocatum Ruiz and Pav.) through the soxhletation method for cytotoxicity test. Maced J Med Sci. 2021; 9: 407-12.
Nordin ML, Abdul Kadir A, Zakaria ZA, Abdullah R, Ab-dullah MN. In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231. BMC Complement Altern Med. 2018; 18: 1-10.
Nuringtyas TR, Pratama Y, Galih G, Wahyuono S, Moeljo-pawiro S. Cytotoxicity of buah merah (Pandanus conoideus Lamk.) extract on breast cancer cell line (T47D). Indonesian J Biotech. 2014; 19: 71-78.
Sakthidhasan P, Kumar PS, Viswanathan MB. Cytotoxic potential of bioactive seed proteins from Mallotus philippensis against various cancer cell lines. Appl Nanosci. 2021; 12: 1-8.
Tai P, Ascoli M. Reactive oxygen species (ROS) play a critical role in the cAMP-induced activation of Ras and the phosphorylation of ERK1/2 in Leydig cells. Mol Endocrinol. 2011; 25: 885-93.
Turker NP, Bakar E. Effects of L-dopa and p-coumaric acid combination on oxidative stress, DNA damage, and mitochondrial apoptosis in neuroblastoma cells. Bangladesh J Pharmacol. 2023; 18: 49-57.
Uddin MN, Afrin R, Josim Uddin M, Uddin MJ, Alam AHMK, Rahman AA. Vanda roxburghii chloroform extract as a potential source of polyphenols with antioxidant and cholinesterase inhibitory activities: Identification of a strong phenolic antioxidant. BMC Complement Altern Med. 2015; 15: 195.
Wu TN, Chen HM, Shyur LF. Current advancements of plant-derived agents for triple-negative breast cancer therapy through deregulating cancer cell functions and reprogram-ming tumor microenvironment. Int J Mol Sci. 2021; 22: 13571.
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