RU.521 protects against neutrophil extracellular traps-induced vascular endothelial injury by inhibiting cGAS/STING pathway

Authors

  • Biao Zhang Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Zaojun Fang Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Ying Lin Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Qian Wu Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Jiaping Chen Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.

DOI:

https://doi.org/10.3329/bjp.v19i2.72930

Keywords:

RU.521; Vascular endothelial injury; Neutrophil extracellular traps, Western blot

Abstract

This study aims to investigate the effect of RU.521 on improving vascular endothelial injury in 14 acute lung injury patients. Serum myeloperoxidase, double-stranded DNA (dsDNA), IFN-β, and TNF-α levels of patients were detected by ELISA. Neutrophil extracellular traps were used to stimulate human venous endothelial cells (HUVEC). cGAS/STING pathway protein was detected by western blot. The protective effect of RU.521 on HUVEC was evaluated by CCK-8. The effects of RU.521 on cGAS/STING pathway expression were detected by PCR and western blot. The levels of myeloperoxidase and (dsDNA) in acute lung injury patients were significantly increased (p<0.05). The expression level of IFN-β in acute lung injury patients was significantly increased (p<0.05). The expression of cGAS and STING proteins in HUVEC cells was significantly increased (p<0.05), and the expression of IFN-β was significantly increased (p<0.05). RU.521 can ameliorate vascular endothelial injury. RU.521 inhibited cGAS/SRING mRNA and protein expression (p<0.05). Thus, neutrophil extracellular traps release dsDNA activates the cGAS/STING pathway-induced vascular endothelial injury in acute lung injury patients. RU.521 protects vascular endothelial injury by inhibiting the cGAS/STING pathway.

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Published

2024-06-15

How to Cite

Zhang, B., Z. Fang, Y. . Lin, Q. Wu, and J. Chen. “RU.521 Protects Against Neutrophil Extracellular Traps-Induced Vascular Endothelial Injury by Inhibiting cGAS/STING Pathway”. Bangladesh Journal of Pharmacology, vol. 19, no. 2, June 2024, pp. 59-64, doi:10.3329/bjp.v19i2.72930.

Issue

Vol. 19 No. 2 (2024)

Section

Research Articles

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Copyright (c) 2024 Biao Zhang, Zaojun Fang, Ying Lin, Qian Wu, Jiaping Chen

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