Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis

Authors

  • Biao Zhang Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China https://orcid.org/0000-0002-0128-6149
  • Junkai Zhao Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Jiamin Yin Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Jiaping Chen Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
  • Lingqi Xu Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.

DOI:

https://doi.org/10.3329/bjp.v19i3.76490

Keywords:

Apoptosis, Ginsenoside Rb1, Immunofluorescence detection, Neutrophil extracellular traps, Vascular endothelial injury

Abstract

The study aims to investigate the effect of ginsenoside Rb1 on improving neutrophil extracellular traps-induced vascular endothelial damage. The CCK8 assay was used to evaluate the impact of ginsenoside Rb1 on cell activity. Flow cytometry was used to assess cell apoptosis. E-cadherin was used to determine the damage to endothelial cells, and caspase 3 was used to detect apoptosis-related proteins. Ginsenoside Rb1 (100 μmol/L) could significantly increase the cell viability (p<0.05). At this concentration, the damage caused by neutrophil extracellular traps to human umbilical vein endothelial cells could be reversed (p<0.05). Ginsenoside Rb1 (100 μmol/L) could significantly inhibit the apoptosis and the expression of caspase3 protein caused by neutrophil extracellular traps (p<0.05). Ginsenoside Rb1 can reduce vascular endothelial injury induced by neutrophil extracellular traps, ginsenoside Rb1 protects vascular endothelium by inhibiting cell apoptosis.

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Published

2024-12-13

How to Cite

Zhang, B., J. Zhao, J. Yin, J. Chen, and L. Xu. “Ginsenoside Rb1 Ameliorates Neutrophil Extracellular Traps-Induced Vascular Endothelial Damage by Suppressing Apoptosis”. Bangladesh Journal of Pharmacology, vol. 19, no. 3, Dec. 2024, pp. 81-87, doi:10.3329/bjp.v19i3.76490.

Issue

Vol. 19 No. 3 (2024)

Section

Research Articles

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Copyright (c) 2024 Biao Zhang, Junkai Zhao, Jiamin Yin, Jiaping Chen, Lingqi Xu

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