Hypoglycemic potentials of Malvaviscus arboreus leaves against alloxanated albino rats
DOI:
https://doi.org/10.3329/bjp.v19i4.79374Keywords:
Malvaviscus arboreus, Phytomedicine, Diabetes Mellitus, Hypoglycemic potential, in vivoAbstract
The study aimed to perform the anti-diabetic potential of leaf extracts of Malvaviscus arboreus in an alloxan-treated albino rat model. Various extracts were prepared by ultrasonication-based successive extraction techniques. Six groups of albino rats (n=5 in each group) induced with alloxan received doses of plant extracts (25 mg/kg) or standard (1 mg/kg) for 14 days. Throughout the study, the blood glucose levels decreased. On day 14, n-hexane and dichloromethane extracts had mean (± SEM) blood glucose levels of 121.2 ± 2.1 and 91.6 ± 1.3 mg/mL respectively, while the extracts of ethyl acetate and methanol resulted in 119.8 ± 3.6 and 114.6 ± 6.1 mg/mL. The histopathological examination of the pancreas revealed reduced inflammation and congestion in the treated groups. Two-way ANOVA showed the level of significance of extracts of dichloromethane p=0.0002, ethyl acetate p=0.0004, methanol p=0.0058 and n-hexane p=0.0003 value. In conclusion, M. arboreus extracts show anti-diabetic activity.
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Abdelhafez OH, Fawzy MA, Fahim JR, Desoukey SY, Krischke M, Mueller MJ, Abdelmohsen UR. Hepatoprotective potential of Malvaviscus arboreus against carbon tetrachloride-induced liver injury in rats. PLoS One. 2018; 13: 1-18.
Abdelhafez OH, Othman EM, Fahim JR, Desoukey SY, Pimentel-Elardo SM, Nodwell JR, Schirmeister T, Tawfike A, Abdelmohsen, UR. Metabolomics analysis and biological investigation of three Malvaceae plants. Phytochem Anal. 2020; 31: 204-14.
Abdul-Hamid M, Moustafa N. Protective effect of curcumin on histopathology and ultrastructure of pancreas in the alloxan treated rats for induction of diabetes. J Basic Appl Zool. 2013; 66: 169-79.
Ahmed D, Kumar V, Verma A, Gupta PS, Kumar H, Dhingra V, Mishra V, Sharma M. Antidiabetic, renal/hepatic/pancreas/cardiac protective and antioxidant potential of methanol/dichloromethane extract of Albizzia lebbeck Benth. stem bark (ALEx) on streptozotocin-induced diabetic rats. BMC Complement Med Ther. 2014; 14: 1-17.
American Diabetes Association. Pharmacologic approaches to glycemic treatment: Standards of medical care in diabetes 2021. Diabetes Care. 2021; 44(Supplement 1): S111-24.
De D, Ali KM, Chatterjee K, Bera TK, Ghosh D. Antihyper-glycemic and antihyperlipidemic effects of n-hexane fraction from the hydro-methanolic extract of sepals of Salmalia malabarica in streptozotocin-induced diabetic rats. J Complement Integr Med. 2012; 9.
De D, Bera TK, Ali KM, Mandal S, Barik B, Ghosh D. Inhibi-tory effects of n-hexane fraction from hydromethanolic (2: 3) extract on α-amylase and α-glucosidase activities of sepal of Salmalia malabarica: An approach through in vitro study. BGM. 2013; 5: 48-53.
Fahim JR, Abdelhafez OH, Abdelmohsen UR, Desoukey SY. GC‒MS analysis of fatty acids of Malvaviscus arboreus leaves. JABS. 2021; 4: 12-15.
Gaikwad SB, Mohan GK, Rani MS. Phytochemicals for dia-betes management. Pharm Crop. 2014; 5: 11-28.
Gazwi HS, Shoeib NA, Mahmoud ME, Soltan OI, Hamed MM, Ragab AE. Phytochemical profile of the ethanol extract of Malvaviscus arboreus red flower and investigation of the antioxidant, antimicrobial, and cytotoxic activities. Antibiotics 2022; 11: 1652.
Gollapalli M, Alansari A, Alkhorasani H, Alsubaii M, Sakloua R, Alzahrani R, Al-Hariri M, Alfares M, AlKhafaji D, Al Argan R. A novel stacking ensemble for detecting three types of diabetes mellitus using a Saudi Arabian dataset: Pre-diabetes, T1DM, and T2DM. Comput Biol Med. 2022; 147: 105757.
González P, Lozano P, Ros G, Solano F. Hyperglycemia and oxidative stress: An integral, updated and critical overview of their metabolic interconnections. Int J Mol Sci. 2023; 24: 9352.
Izu GO, Mashele SS, Chukwuma CI. Investigating the phytochemistry and underlying glycemic control mechanisms of Litchi chinensis Sonn. (Litchi) peel ethyl acetate extract in a fructose/streptozotocin diabetic model of rats. Nutrients 2024; 16: 3644.
Kannan M, Thamaraiselvi S, Uma D. Extraction of phenolic compounds and assessing antioxidant activity of Malvaviscus arboreus Cav flowers. J Pharmacogn Phytochem. 2018; 7: 1261-63.
Khanra R, Dewanjee S, K Dua T, Sahu R, Gangopadhyay M, De Feo V, Zia-Ul-Haq M. Abroma augusta L. (Malvaceae) leaf extract attenuates diabetes induced nephropathy and cardiomyopathy via inhibition of oxidative stress and inflammatory response. J Transl Med. 2015; 13: 1-14.
Lim, T. Malvaviscus arboreus. In: Edible medicinal and non-medicinal plants. Volume 8. Springer, 2014, pp 405-08.
Lin L, Zhang Y. Chemical constituents and anti-diabetic activity of dichloromethane extract from Ficus carica leaves. Diabetes Metab Syndr Obes. 2023; 5: 979-91.
Mardiah M, Zakaria F, Prangdimurti E, Damanik R. The effect of roselle extract (Hibiscus sabdariffa Linn.) on blood glucose level and total antioxidant level on diabetic rat induced by streptozotocin. IOSR J Pharm. 2014; 4: 8-16.
Mohammed DM, Elsayed N, Abou Baker DH, Ahmed KA, Sabry BA. Bioactivity and antidiabetic properties of Malva parviflora L. leaves extract and its nano-formulation in streptozotocin-induced diabetic rats. Heliyon 2022; 8.
Odoh U, Onugha V, Chukwube V. Evaluation of antidiabetic effect and hematotological profile of methanol extract of Ceiba pentandra G (Malvaceae) stem bark on alloxan-induced diabetic rats. Afr J Pharm Pharmacol. 2016; 10: 584-90.
Okokon JE, Davies K, John L, Iwara K, Li WW, Thomas PS. Phytochemical characterization, antihyperglycaemic and antihyperlipidemic activities of Setaria megaphylla in alloxan-induced diabetic rats. Phytomed Plus. 2022; 2: 100182.
Olaokun OO, McGaw LJ, Rensburg IJV, Eloff JN, Naidoo V. Antidiabetic activity of the ethyl acetate fraction of Ficus lutea (Moraceae) leaf extract: Comparison of an in vitro assay with an in vivo obese mouse model. BMC Complement Altern Med. 2016; 16: 1-12.
Prabhakar PK, Doble M. Mechanism of action of natural products used in the treatment of diabetes mellitus. Chin J Integr Med. 2011; 17: 563-74.
Rajendiran D, Packirisamy S, Gunasekaran K. A review on role of antioxidants in diabetes. Asian J Pharm Clin Res. 2018; 11: 48-53.
Rodríguez-García CM, Ruiz-Ruiz JC, Peraza-Echeverría L, Peraza-Sánchez SR, Torres-Tapia LW, Pérez-Brito D, Tapia-Tussell R, Herrera-Chalé FG, Segura-Campos MR, Quijano-Ramayo A. Antioxidant, antihypertensive, antihyperglycemic, and antimicrobial activity of aqueous extracts from twelve native plants of the Yucatan coast. PLoS One. 2019; 14.
Rodríguez-Morales S, Ocampo-Medina B, Romero-Ceronio N, Alvarado-Sánchez C, Vilchis-Reyes MÁ, Roa de la Fuente, LF, Ortiz-Andrade R, Hernández-Abreu O. Metabolic profiling of vasorelaxant extract from Malvaviscus arboreus by LC/QTOF-MS. Chem Biodivers. 2021; 18.
Sarian MN, Ahmed QU, Mat So’ad SZ, Alhassan AM, Murugesu S, Perumal V, Syed Mohamad SNA, Khatib A, Latip J. Antioxidant and antidiabetic effects of flavonoids: A structure-activity relationship based study. BioMed Re Int. 2017; 2017.
Sen PB, Bhattacharya G. Reversal of the diabetogenic action of alloxan by sulfhydryl compounds. Science 1952; 115: 41-43.
Singh S, Dodiya TR, Singh S, Dodiya R. Topical wound healing, antimicrobial and antioxidant potential of Mimosa pudica Linn root extracted using n-hexane followed by methanol, fortified in ointment base. Int J Pharm Sci Nanotechnol. 2021; 14: 5472-80.
Slaoui M, Fiette L. Histopathology procedures: From tissue sampling to histopathological evaluation. Drug safety evaluation: Methods Protoc. 2011; 69-82.
Yeasmin Z, Tanvir S, Sharmin T, Rashid RB, Sikder MA A, Rashid MA. Bioactivities of Malvaviscus arboreus var. drum-mondii and Phyllanthus reticulatus Poir. Dhaka Univ J Pharm Sci. 2014; 13: 143-47.
Yin MC. Inhibitory effects and actions of pentacyclic triterpenes upon glycation. Biomedicine. 2015; 5: 1-8.
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