Increased DNA damage in blood cells of rat treated with lead as assessed by comet assay

Authors

  • Mohammad Arif Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka
  • Yearul Kabir Department of Family Sciences, College for Women, Kuwait University
  • Faizule Hassan Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka
  • T. M. Zaved Waise Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka
  • Md. Ehsanul Hoque Mazumder Department of Pharmacy, Jahangirnagar University, Dhaka
  • Shafiqur Rahman Department of Pharmaceutical Sciences, South Dakota State University

DOI:

https://doi.org/10.3329/bjp.v3i2.948

Keywords:

DNA damage, Lead toxicity, Oxidative stress

Abstract

A growing body of evidence suggests that oxidative stress is the key player in the pathogenesis of lead-induced toxicity. The present study investigated lead induced oxidative DNA damage, if any in rat blood cells by alkaline comet assay. Lead was administered intraperitoneally to rats at doses of 25, 50 and 100 mg/kg body weight for 5 days consecutively. Blood collected on day six from sacrificed lead-treated rats was used to assess the extent of DNA damage by comet assay which entailed measurement of comet length, olive tail moment, tail DNA (%) and tail length. The results showed that treatment with lead significantly increased DNA damage in a dose-dependent manner. Therefore, our data suggests that lead treatment is associated with oxidative stress-induced DNA damage in rat blood cells which could be used as an early bio-marker of lead-toxicity.

Downloads

Download data is not yet available.
Abstract
197
Download
110 Read
5

Author Biographies

Mohammad Arif, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka

Assistant professor

Yearul Kabir, Department of Family Sciences, College for Women, Kuwait University

Assistant Professor

Downloads

Additional Files

Published

2008-07-15

How to Cite

Arif, M., Y. Kabir, F. Hassan, T. M. Z. Waise, M. E. H. Mazumder, and S. Rahman. “Increased DNA Damage in Blood Cells of Rat Treated With Lead As Assessed by Comet Assay”. Bangladesh Journal of Pharmacology, vol. 3, no. 2, July 2008, pp. 97-101, doi:10.3329/bjp.v3i2.948.

Issue

Vol. 3 No. 2 (2008)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).