Effects of the crude and the n-hexane extract of Nigella sativa Linn.(kalajira) upon diabetic rats
DOI:
https://doi.org/10.3329/bjp.v4i1.951Keywords:
Diabetes, Nigella sativa, StreptozotocinAbstract
The study was carried out to investigate into the effect of concomitant administration of the crude and the n-hexane extract of Nigella sativa (kalajira) upon streptozotocin-induced diabetic adult male rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin on day 1 while crude nigella powder in deionized water and the n-hexane extract of nigella were administered orally concomitantly from day 1 to 21. Rats were sacrificed on day 22. The serum glucose and cholesterol concentrations that were elevated in diabetic rats were normalized or near normalized by the crude nigella or the n-hexane extract administration; while the elevated serum triglyceride concentrations of the diabetic rats were brought down to lower than control values. The pancreatic GSH was closer to control value, and pancreatic histology suggested reappearance of β cells. The crude nigella concomitant to streptozotocin (STZ + Nc) administration appeared to provide better alleviation compared to the n-hexane extract of nigella concomitant to streptozotocin (STZ + H) administration.
Downloads
198
133 Read
4
References
Ara N. Antimicrobial activity of the volatile oil of Nigella sativa Lnn. seeds. M.Phil Thesis, Dhaka, Bangabandhu Sheikh Mujib Medical University, 1999.
Begum NA, Dewan ZF, Nahar N, Mamun MIR. Effect of n-hexane extract of Nigella sativa on gentamicin-induced nephrotoxicity in rats. Bangladesh J Pharmacol. 2008; 1: 16-20.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82: 70-77.
Khan N, Sharma S, Sultana S. Nigella sativa (black cumin) ameliorates potassium bromate induced early events of carcinogenesis: Diminution of oxidative stress. Hum Exp Toxicol. 2003; 22: 193-203.
Rashid MA, Rahman S, Choudhury SAR, Ahmed M, Chowdhury AKA. Primary screening of some herbal products of Bangladesh for antihypertensive activity. Bangladesh J Neuro. 1987; 3: 56-58.
Steward HK. Manual of histologic and special staining techniques. New York: Mc Graw-Hill Book Company Inc., 1960.
Saha RR, Dewan ZF, Uddin N. Effects of Nigella sativa Linn. (Kalajira) on serum lipid profile of hyperlipidemic rats. Bangladesh J Physiol Pharmacol. 2004; 20: 36-38.
Trinder P. Determination of the blood glucose using an oxidase peroxidase system with a noncarcinogenic chromogen. J Clin Path. 1969; 22: 158-61.
Uddin N, Dewan ZF, Zaman M, Saha RR, Sultana M. Effects of Nigella sativa Linn. (kalajira) on serum glucose concentration in streptozotocin-induced diabetic rats. Bangladesh J Physiol Pharmacol. 2002; 18: 6-9.
Uddin N, Dewan ZF, Zaman M, Nasir M, Moshiuzzaman, Saha RR. Comperative study of hypoglycemic effects of crude Nigella sativa Linn. (kalajira) and its ethanol extract on STZ-induced diabetic rats. J Med Sci Res. 2005; 5: 27-32.
Additional Files
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).