Chemical composition and antimicrobial properties of different basil essential oils chemotypes from Togo
DOI:
https://doi.org/10.3329/bjp.v4i1.998Keywords:
Anethole, Antimicrobial, Essential oils, Estragole, Linalool, Methyeugenol, Ocimum basilicumAbstract
The aerial parts essential oils of Ocimum basilicum (Lamiaceae) from Togo were steam-distilled and investigated for their percentage composition (GC and GC/MS) and in vitro antimicrobial activities. Five oil chemotypes were identified and classified as follows in line with their principal components: estragole type; linalool/estragole type; methyleugenol type; methyleugenol/t-anethole type; t-anethole type. The in vitro microbiological experiments revealed that only the methyleugenol and methyleugenol/t-anethole chemotypes were active against tested fungi and bacteria. Their minimum inhibitory concentrations (MIC) ranged from 80 to 150 µL/L and from 200 to 500 µL/L respectively. Likewise, on tested bacteria the MIC varied from 200 µL/L to 400 µL/L and from 250 to 500 µL/L respectively. These findings are supportive of the potential of both basil oil chemotypes for use as active ingredients in natural antibiotic drugs.
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References
Adams RP. Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Carol Stream, Ilinois, Allured Publishing Corporation, 2001.
Adjanohoun E, Ahyi MRA, Aké Assi L, Akpagana K, Chibon P, El-Hadj A, Eymen I, Goutote E, Ginko S, Hodouto KK, Hougnon P, Keita A, Kéoula Y, Klouga-Ocloo WP, Lo I, Siamevi K, Taffame KK, Garba M, Gassita JN, Gbeassor M. Médecine traditionnelle et Pharmacopée: Contribution aux études ethnobotaniques et floristiques du Togo. ACCT, Paris, 1986.
Albuquerque UP. de Taxonomia e ethnobotânica do gênero Ocimum L. (Lamiaceae) no Nordeste do Brasil-renfêrencia especial para Pernambuco. Recife. Dissertação (Mestrado em Biologia Vegetal)- Centro de Ciencias Biológicas, Universidade Federal de Pernambuco, 1996, p 125.
Archtander S. Perfume and flavour material of natural origin. Carol Steam, Allured Publishing Corporation, 1994, p 81.
Alvarez P, Enriquez AM, Toro C, Martinez I, Buhigas I, de Miguel S, Lago M, Puente S, del Palacio A, Baquero M. Dermatomiucosis de importación por Scytalidium dimidiatum: A propósito de tres casos. Rev Iberoam Micol. 2000; 17: 10206.
Baba-Moussa F, Koumaglo K, Ayédoun K, Akpagana K, Moudachirou M, Bouchet P. Activité antifongique dhuiles essentielles extraites au Bénin et au Togo. Cryptogamie, Mycol. 1997; 18: 165-68.
Baritaux O, Richard H, Touche J, Dersbesy M. Effects of drying and storage of herbs and spices on the essential oil. Part I. Basil, O. basilicum L. Flav Fragr J. 1992; 7: 227-71.
Benjilali B, Tantaoui-Elaraki A, Ismaïlou-Alaoui M, Ayadi A. Méthode d'étude des propriétés antiseptiques des huiles essentielles par contact direct en milieu gélosé. Plant Méd Phytother. 1986; 20: 15567.
Berrada M, Ait Igri M, Il Idrissi A, Bellakdar. Contribution à lanalyse de lhuile essentielle dun cultivar dOcimum basilicum L. du Maroc. Al Buruniya, Rev Mar Pharm. 1987; 3: 137-44.
Bergogne-Bérézin E. The increasing significance of outbreaks of Acinetobacter spp.: The need for control and new agents. J Hosp Infect. 1995; 30: 441-52.
Chaumont JP, Leger D. Propriétés antifongiques de quelques phénols et de composés chimiquement très voisins. Relations structure-activité. Plant Med Phytother. 1989; 23: 124-28.
Chaumont JP, Mandin D, Sanda K, Koba K, De Souza C. Activités antimicrobiennes de cinq huiles essentielles de lamiacées togolaises vis à vis de germes représentatifs de la microflore cutanée. Acta Bot Gallica. 2001; 148: 93-101.
Center for Disease Control and Prevention (CDC). National nosocomial infections surveillance system report. Data sumary from January 1990-May 1990. Am J Infect Control 1999; 27: 520-32.
Craveiro AA, Matos FJ, Alencar JW. A simple and inexpensive steam generator for essential oils extraction. J Chem Ed. 1976; 53: 652.
Darrah H. Investigation on cultivars of Basils (Ocimum). Econ Bota. 1974; 28: 63-67.
Ekundayo O, Laakso I, Oguntimein B, Okogun JL, Elujoba A, Hulbinen A. Essential oil of Ocimum basilicum from Nigeria. Acta Pharm Fen. 1987; 96: 101-06.
Friedman M, Henika PR, Mandrell RE. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J Food Prot. 2002; 65: 1545-60.
Griffin SG, Markham JL, Leach DN. An agar dilution method for the determination of the minimum inhibitory concentration of essential oils. J Essent Oil Res. 2000; 12: 249-55.
Guenther E. The essential oils. Vol. 2. New York, Van Nostrand, 1949, pp 16-18, 503-05.
Kayibou G. Les Eucalyptus du Congo. Variations inter et intra-spécifiques du rendements et de la composition de leurs huiles essentielles. Thèse de doctorat Université de Montpellier II, France, 1992, p 112.
Ketoh KG, Glitoh AI, Huignard J. Susceptibility of the bruchus Callosobruchus maculatus (Coleoptera: Bruchidae) and its parasitoid Dinarmus basalis (Hymenopterae: Pteromatidae) to three essential oils. J Econ Entomol. 2002; 95: 174-82.
Keita SM, Vincent C, Schmit J, Arnason JT, Belanger A. Efficacy of oil of Ocimum basilicum L. and O. gratissimum L. applied as an insecticidal fumigant and powder to control Callosobruchus maculatus (Fab). J Stored Prod Res. 2001; 37: 339-49.
Kondjoyan N, Berdagué JL. A compilation of relative retention indices for the analysis of aromatic compounds. Ed. Laboratoire Flaveur, INRA de Theix, France, 1996.
Koumaglo K, Dotse K, Akpagana K, Garneau FX, Gagnon H, Jean IF, Moudachirou M, Addae-Mensah I. Analyse des huiles essentielles de deux plantes aromatiques du Togo. Riv Ital EPPOS. 1996; 7: 680-91.
Marshall J, Leening JP, Holland KT. The cutaneous microbiology of normal human feet. J Appl Bacteriol. 1987; 62: 39-46.
Marshall J, Holland KT, Gribbon EM. A comparative study of cutaneous microflora of normal feet with low and high levels of odour. J Appl Bacteriol. 1988; 65: 61-68.
Moudachirou M, Ayedou MA, Gbenou J, Garneau FX, Koumaglo KH, Mensah-Addae I. Composition chimique des huiles essentielles des feuilles de Clausena anisata récoltées dans la sous-region Bénin-Togo-Ghana. J Soachim. 1997; 3: 49-54.
Okunde AL, Olaifa JL. Estragole: An acute toxic principle from the volatile oil of the leaves of Clausena anisata. J Nat Prod. 1987; 50: 990-91.
Ouaffak L, Gati A, Lyagoubi M. Les teignes du cuir chevelu dans les écoles Primaires de Khermisset (Maroc). J Mycol Méd. 2001; 114: 181-84.
Paton A. A synopsis of Ocimum L. (Labiatae) in Africa. Kew Bull. 1992; 47: 403-36.
Rennie PJ, Gower DB, Holland KT. In vitro studies of human axillary odour and cutaneous microflora. Br J Dermatol. 1991; 124: 596-602.
Sanda K, Koba K, Nambo P, Gaset A. Chemical investigation of Ocimum species growing in Togo. Flav Fragr J. 1998; 13: 226-32.
Vandemeulebroucke BT, Mounkassa B, Deloye J, Jousserand P, Poujade F, Petithory JC. Teignes du cuir chevelu en milieu scolaire rural au Mali. J Mycol Med. 1999; 92: 11-13.
Viollon C, Chaumont JP. Antifungal properties of essential oils and their main components upon Cryptococcus neoformans. Mycopathologia 1994; 128: 151-53.
Yayi E, Moudachirou M, Chalchat JC. Chemotyping of three Ocimum species from Benin: O. basilicum, O. canum and O. gratissimum . J Essent Oil Res. 2001; 13: 13-17.
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