Application of internal transcribed spacer of nuclear ribosomal DNA for identification of <i>Echinops mandavillei</i> Kit Tan

Authors

  • Fahad M.A. Al-Hemaid Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451
  • M. Ajmal Ali Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451
  • Joongku Lee International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305 806
  • Gábor Gyulai Institute of Genetics and Biotechnology, St. István University, Gödöllo H-2103
  • Arun K. Pandey Department of Botany, University of Delhi, Delhi 110007

DOI:

https://doi.org/10.3329/bjpt.v21i1.19256

Keywords:

Echinops mandavillei, Asteraceae, ITS, nrDNA, Endemic, Saudi Arabia

Abstract

The present study explored the use of internal transcribed spacers (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA (nrDNA) for identification of Echinops mandavillei Kit Tan, an endemic species to Saudi Arabia. The sequence similarity search using Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses of the ITS sequence of E. mandavillei Kit Tan showed high level of sequence similarity (98%) with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. mandavillei could have a potential use for molecular genotyping.

DOI: http://dx.doi.org/10.3329/bjpt.v21i1.19256

Bangladesh J. Plant Taxon. 21(1): 33-42, 2014 (June)

Downloads

Download data is not yet available.
Abstract
267
PDF
233

Downloads

Published

2014-06-23

How to Cite

Al-Hemaid, F. M., Ali, M. A., Lee, J., Gyulai, G., & Pandey, A. K. (2014). Application of internal transcribed spacer of nuclear ribosomal DNA for identification of <i>Echinops mandavillei</i> Kit Tan. Bangladesh Journal of Plant Taxonomy, 21(1), 33–42. https://doi.org/10.3329/bjpt.v21i1.19256

Issue

Vol. 21 No. 1 (2014)

Section

Articles

License

Copyright on any research article is transferred in full to Bangladesh Association of Plant Taxonomists upon publication in Bangladesh Journal of Plant Taxonomy. The copyright transfer includes the right to reproduce and distribute the article in any form of reproduction (printing, electronic media or any other form). 

Creative Commons Licence
Articles in the Bangladesh Journal of Plant Taxonomy are Open Access articles published under the Creative Commons CC BY License Creative Commons Attribution 4.0 International License. 

This license permits use, distribution and reproduction in any medium, provided the original work is properly cited. It is author's responsibility to obtain the permission from appropriate authority if figures are reused from a previously published document.