Morphological and Molecular Characterization Of Micromycetes Associated With Seeds of Selected Cotton (Gossypium Hirsutum L.) Varieties

Authors

  • Amina Khatun Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh
  • Shamim Shamsi Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh
  • Ma Bashar Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh

DOI:

https://doi.org/10.3329/bjpt.v29i2.63531

Keywords:

Mycoflora; Cotton varieties; ITS; PCR amplification; Sequence analysis

Abstract

A total of 14 varieties (CB1-CB14) of cotton (Gossypium hirsutum L.) seeds were collected from Cotton Research, Training and Seed multiplication Farm, Sreepur, Gazipur to detect and identify the seed borne fungi by morphological and molecular techniques. The sequence results obtained using the ITS1 and ITS4 primers were compared with NCBI GenBank and BOL database using BLAST analysis. In the present investigation, a total of 29 fungal isolates were morphologically identified from different varieties of cotton seeds, of which 19 fungal isolates were identified by molecular techniques. Among the isolated fungi, Aspergillus subramanianii, A. toxicarius, A. wentii, Penicillium aculeatum, P. citrinum, Rhizomucor sp. and Meyerozyma guilliermondii have been reported as new records for Bangladesh.

Bangladesh J. Plant Taxon. 29(2): 297-312, 2022 (December)

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Published

2022-12-27

How to Cite

Khatun, A. ., Shamsi, S. ., & Bashar, M. . (2022). Morphological and Molecular Characterization Of Micromycetes Associated With Seeds of Selected Cotton (Gossypium Hirsutum L.) Varieties. Bangladesh Journal of Plant Taxonomy, 29(2), 297–312. https://doi.org/10.3329/bjpt.v29i2.63531

Issue

Vol. 29 No. 2 (2022)

Section

Articles

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Copyright (c) 2022 Bangladesh Association of Plant Taxonomists

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