New Record of Scenedesmus Vacuolatus From Soil In Vojvodina, Serbia

Authors

  • Timea Hajnal Jafari University of Novi Sad, Faculty of Agriculture, Dositeja Obradovića Sq.8, Novi Sad, Serbia
  • Vladimira Žunić University of Novi Sad, Faculty of Agriculture, Dositeja Obradovića Sq.8, Novi Sad, Serbia
  • Simonida Djurić University of Novi Sad, Faculty of Agriculture, Dositeja Obradovića Sq.8, Novi Sad, Serbia
  • Dragana Stamenov University of Novi Sad, Faculty of Agriculture, Dositeja Obradovića Sq.8, Novi Sad, Serbia

DOI:

https://doi.org/10.3329/bjpt.v30i2.70501

Keywords:

Soil algae; Scenedesmus vacuolatus; DNA extraction; ITS2.

Abstract

Soil algae are photosynthetic microorganisms showing huge differences in morphology depending on the soil type, agricultural practices, and environmental factors. The aim of this research was to compare the results of classical and molecular protocols for microalgal identification. The green microalgae were isolated from acidic arable soil of dystric cambisol type, using BG11 medium and the agar plate method. Firstly, the identification of the microalgae was performed based on its morphological characteristics using light microscopy (LM) and taxonomic monographs and reference books. The isolate was initially annotated on genus level as Coelastrella sp. Gene marker (ITS2) was used for molecular identification. According to this protocol, the microalga was identified as Scenedesmus vacuolatus. Combination of morphology and DNA-based approach proved to be the most effective for obtaining a consistent species level identification. Moreover, this is the first record of Scenedesmus vacuolatus identification from soil in Vojvodina, Serbia.

Bangladesh J. Plant Taxon. 30(2): 255-261, 2023 (December)

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Published

2023-12-24

How to Cite

Jafari, T. H. ., Žunić, V. ., Djurić , S. ., & Stamenov, D. . (2023). New Record of Scenedesmus Vacuolatus From Soil In Vojvodina, Serbia. Bangladesh Journal of Plant Taxonomy, 30(2), 255–261. https://doi.org/10.3329/bjpt.v30i2.70501

Issue

Vol. 30 No. 2 (2023)

Section

Articles

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