@article{Hassan_Khatun_2011, title={<i>In Vitro</i> Shoot Proliferation and Plant Regeneration of <i>Phyllanthus fraternus</i> Webster (B. Bhuiamla), a Seasonal Medicinal Herb}, volume={46}, url={https://banglajol.info/index.php/BJSIR/article/view/8187}, DOI={10.3329/bjsir.v46i2.8187}, abstractNote={&lt;p&gt;An efficient system was developed for shoot proliferation and large scale plant regeneration of a seasonal multipotent medicinal herb, &lt;em&gt;Phyllanthus fraternus &lt;/em&gt;Webster through &lt;em&gt;in vitro &lt;/em&gt;culture. Shoot tips and nodal explants of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 88% of nodal explants responded to produce maximum number (16.8 &amp;plusmn; 0.95) of shoots per culture. &lt;em&gt;In vitro &lt;/em&gt;raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Key words&lt;/strong&gt;: &lt;em&gt;Phyllanthus fraternus&lt;/em&gt;; Medicinal plant; Shoot proliferation; Regeneration; Acclimatization&lt;/p&gt; &lt;p&gt;DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8187&lt;/p&gt; &lt;p&gt;&lt;em&gt;Bangladesh&lt;/em&gt;&lt;em&gt; J. Sci. Ind. Res. &lt;/em&gt;&lt;strong&gt;46(2)&lt;/strong&gt;, 205-210, 2011&lt;/p&gt;}, number={2}, journal={Bangladesh Journal of Scientific and Industrial Research}, author={Hassan, AKM Sayeed and Khatun, R}, year={2011}, month={Aug.}, pages={205–210} }