TY - JOUR AU - Ahmed, Firoj AU - Akter, Dilruba AU - Muhit, Md. Abdul AU - Raihan, Sheikh Zahir AU - Faroque, A B M PY - 2018/11/22 Y2 - 2024/03/28 TI - DPPH Free-radical Scavenging and Cytotoxic Activities of Leeamacrophylla JF - Bangladesh Medical Research Council Bulletin JA - Bangladesh Med Res Counc Bull VL - 44 IS - 2 SE - Research Papers DO - 10.3329/bmrcb.v44i2.38690 UR - https://banglajol.info/index.php/BMRCB/article/view/38690 SP - 77-81 AB - <p>                        Structural damage inflicted to a wide variety of bioorganic compounds (DNA, protein, carbohydrates, and lipid) may occur as a consequence of oxidative damage which is naturally attributed to our daily lifestyle. A number of mechanisms are exploited to neutralize the reactive oxygen species. Medicinal plant can play a pivotal role of detoxifying the generated ROS. <em>Leeamacrophylla</em> (Leeaceae), a wild medicinal plant, has been taken into consideration of having antioxidant and cytotoxic properties. In this study, <em>in vitro </em>DPPH free radical scavenging activity, total phenolic content and total flavonoid content of methanol extract from the leaves of <em>L. macrophylla </em>were determined with using UV spectrophotometric method. Antioxidant activity of the crude extract was expressed as percentage of DPPH free radical inhibition and IC<sub>50</sub> value (μg/ml). The IC<sub>50</sub> value of <em>L. macrophylla</em> was found to be 152.40μg/ml. Compared to standard ascorbic acid (4.36 μg/ml), <em>L. macrophylla</em> leaf extract has moderate antioxidant property. The total phenolic content was 65.75 mg/g of dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentration was 59.8 mg/g, expressed as quercetin equivalents. In the brine shrimp lethality test, LC<sub>50</sub>value of crude methanolic extract obtained from the best-fit line slope was 3.06μg/ml which was significant comparing with control (vincristine sulphate). Above results suggested that the <em>L. macrophylla</em> can be regarded as a promising candidate for natural plant sources of antioxidants with high value.</p> ER -