TY - JOUR AU - Barua, Aruna AU - Kuddus, Md Ruhul AU - Chowdhury, M Mohi Uddin AU - Rashid, Mohammad A AU - Ibrahim, Mohammed PY - 2023/01/31 Y2 - 2024/03/28 TI - Antioxidant, Anti-inflammatory, Antimicrobial and Thrombolytic Activities of Eclipta alba L. Growing in Bangladesh JF - Bangladesh Pharmaceutical Journal JA - Bangla Pharma J VL - 26 IS - 1 SE - Articles DO - 10.3329/bpj.v26i1.64214 UR - https://banglajol.info/index.php/BPJ/article/view/64214 SP - 20-27 AB - <p><em>Eclipta alba </em>L. (Family: Asteraceae) is an evergreen herb of ethnomedicinal significance in the Indian subcontinent. The present study was carried out to determine the <em>in vitro </em>antioxidant, anti-inflammatory, antimicrobial and thrombolytic activities of <em>n</em>-hexane, chloroform and aqueous soluble fractions of ethanol crude extract of <em>E. alba </em>whole plant. Preliminary phytochemical screening was performed by qualitative tests which revealed the presence of alkaloids, steroids, glycosides, tannins, flavonoids, saponins and amides in <em>E. alba</em>. During the DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assay, the aqueous soluble fraction of <em>E. alba </em>at a dose of 100 μg/ml showed maximum 87.5% inhibition in scavenging the DPPH radical with IC50 value of 7.86 μg/ml. Anti-inflammatory activity was evaluated by egg albumin denaturation technique and RBC membrane stabilizing method. In both experiments, all the test samples of <em>E. alba </em>showed a concentration-dependent anti-inflammatory potential while the aqueous soluble fraction at the dose of 500 μg/ml demonstrated the maximum 90.12% inhibition of egg albumin denaturation and 78.42% inhibition of RBC membrane hemolysis against hypotonic solution. Antimicrobial sensitivity was evaluated by disc diffusion assay against several bacterial and fungal species. All the test samples at a concentration of 500 μg/disc exhibited noticeable antibacterial (zone of inhibition = 11-23 mm) and antifungal (zone of inhibition = 10-21 mm) activities when compared to the respective standard drug. The aqueous soluble fraction showed maximum activity against <em>Lactobacillus casai </em>(zone of inhibition = 23 mm). During antifungal test, the maximum activity was exhibited by the <em>n</em>-hexane fraction against <em>Pityrosporum ovale </em>(zone of inhibition = 21 mm). During <em>in vitro </em>thrombolytic assay, the aqueous soluble fraction revealed 56.84% lysis of blood clot, as compared to the standard streptokinase (84.60%). The results of our study suggest that <em>E. alba </em>can be considered for further investigation in order to discover the pharmacologically active natural products.</p><p>Bangladesh Pharmaceutical Journal <strong>26</strong>(1): 20-27, 2023 (January)</p> ER -