GGE Biplot Analysis for Yield Stability in Multi-environment Trials of Promising Hybrid Rice (Oryza sativa L.)

Abstract

The genotype and genotype by environment biplot model is an excellent tool for visual multi-environment trials data analysis. In this study we investigated grain yield of six rice genotypes (three tested, one released hybrids and two inbred check varieties) in five environments. The combined analysis of variance for grain yield data indicated that the differences among all sources of variation were highly significant (P<0.001). Environment (E), Genotype (G) and G × E interaction effects accounted for 12.49, 76.51 and 10.21% of the total sum of squares respectively. The first two principal components (PC1 and PC2) were used to display a two-dimensional GGE biplot. Thus, genotypic PC1 scores>0 classified the high yielding genotypes while PC1 scores<0 identified low yielding genotypes. Unlike genotypic PC1, genotypic PC2 scores discriminated the unstable ones. The GGE biplot analysis was useful in identifying stable genotypes with high yield performance. In this study, the polygon view of GGE biplot showed that the vertex genotypes were BRRI1A/BR168R (G1), BRRI10A/BRRI10R (G2) and BRRI dhan28 (G5) having the largest distance from the origin, which was most discriminated genotypes with the unstable ones. These vertex genotypes BRRI1A/BR168R (G1) and BRRI10A/BRRI10R (G2) gave higher yield (PC1 scores>0) while another vertex genotype BRRI dhan28 (G5) produced low yield (PC1 score<0). Hence, the vertex genotype BRRI10A/BRRI10R (G2) was high yielding for all environments and it fell into section 1 following IR58025A/BRRI10R (G3) and BRRI hybrid dhan1 (G4). Mean yield and stability performance over environments of each genotype is explored by using the average environment (tester) coordinate (AEC) methods. These methods show that the genotypes BRRI10A/BRRI10R (G2), IR58025A/BRRI10R (G3) and BRRI hybrid dhan1 (G4) had higher stability as well as higher mean yield while the genotype IR58025A/BRRI10R (G3) had the highest stability out of these three genotypes. The ideal genotype biplot suggests that the closer to ideal genotype was IR58025A/BRRI10R (G3) followed by G2 and G4 being more desirable than the other genotypes. Similarly, the environment Barisal (E3) was ideal environment followed by E1 (Gazipur), E2 (Comilla) and E5 (Satkhira). Hence, the environment Barisal (E3) is more stable and suitable for all genotypes following Satkhira (E5) because it has large PC1 and small PC2 score but Rangpur (E4) is a discriminating environment because it has large PC2 score. The interrelationship among the environments according to the small angles of test environments was highly positively correlated. Gazipur (E1), Comilla (E2), Barisal (E3) and Satkhira (E5) were closely correlated with small angles but Rangpur (E4) had medium long angles. Comparison between two genotypes showed that BRRI10A/BRRI10R (G2) and IR58025A/BRRI10R (G3) were high yielder in test environments. Thus, the difference between G2 and G3 was relatively small in test environments.

Bangladesh Rice j. 2015, 19(1): 1-8